Title:
HIGH TEMPERATURE RESISTANT MANNANASE MUTANT
Document Type and Number:
WIPO Patent Application WO/2023/041040
Kind Code:
A1
Abstract:
A high temperature resistant mannanase mutant, comprising at least one mutation site among D49C/I/N, G58D/E/S, H90L, T99Y, K120Y, N124P, T130D/N/P/Q/R/S, Y132F, S136M, S140L/K, T148K, F206Y, A212N/S/P, L257F, S263R, D268A/E/G/P, G273P, S302M/E, S306M/Q, T315P, T353L/F, D360E/N/K, and A362R. The high temperature resistant mannanase mutant has heat resistance significantly higher than the wild type mannanase, and can be widely used in the field of feeds.
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Inventors:
WU XIUXIU (CN)
LI RUI (CN)
LI YUQIANG (CN)
HUANG YIJUN (CN)
LI XINPEI (CN)
XU LIHONG (CN)
SONG QINGQING (CN)
XIN WEN (CN)
LI RUI (CN)
LI YUQIANG (CN)
HUANG YIJUN (CN)
LI XINPEI (CN)
XU LIHONG (CN)
SONG QINGQING (CN)
XIN WEN (CN)
Application Number:
PCT/CN2022/119328
Publication Date:
March 23, 2023
Filing Date:
September 16, 2022
Export Citation:
Assignee:
QINGDAO VLAND BIOTECH GROUP CO LTD (CN)
International Classes:
C12N9/42; C12N1/19; C12N15/56; C12N15/81; C12R1/84
Domestic Patent References:
| WO2021152123A1 | 2021-08-05 | |||
| WO2019214702A1 | 2019-11-14 |
Foreign References:
| CN103525790A | 2014-01-22 | |||
| CN110577946A | 2019-12-17 | |||
| CN111363735A | 2020-07-03 | |||
| CN104726481A | 2015-06-24 | |||
| CN103255119A | 2013-08-21 | |||
| CN110669748A | 2020-01-10 | |||
| CN111117987A | 2020-05-08 |
Other References:
LUO CHANGCAI, MIAO JING , LI GUO-YING, DU YAO , YU XIAO-BIN: "Screening and Identification of a Strain Highly Producing Thermostable β-Mannanase", LETTERS IN BIOTECHNOLOGY, JUNSHI YIXUE KEXUEYUAN, CN, vol. 29, no. 2, 30 March 2018 (2018-03-30), CN , pages 233 - 257, XP093050047, ISSN: 1009-0002, DOI: 10.3969/j.issn.1009-0002.2018.02.015
ZHOU HAIYAN, WU YONGYAO: "Effect of Point Mutation on Structure and Function of Bacillus Subtilis Mannanase Structure", JOURNAL OF HIGHER CORRESPONDENCE EDUCATION(NATURAL SCIENCES), vol. 22, no. 1, 26 February 2008 (2008-02-26), pages 9 - 12, XP093050051, ISSN: 2095-5987
ZHOU HAIYAN, WU YONGYAO: "Effect of Point Mutation on Structure and Function of Bacillus Subtilis Mannanase Structure", JOURNAL OF HIGHER CORRESPONDENCE EDUCATION(NATURAL SCIENCES), vol. 22, no. 1, 26 February 2008 (2008-02-26), pages 9 - 12, XP093050051, ISSN: 2095-5987
Attorney, Agent or Firm:
UNITALEN ATTORNEYS AT LAW CO., LTD. (CN)
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