Title:
HIGH-THROUGHPUT SCREENING OF EXPRESSED DNA LIBRARIES IN FILAMENTOUS FUNGI
Document Type and Number:
WIPO Patent Application WO2001079558
Kind Code:
A8
Abstract:
The invention provides a method for the expression of exogenous DNA libraries in filamentous fungi. The fungi are capable of processing intron-containing eukaryotic genes, and also can carry out post-translational processing steps such as glycosylation and protein folding. The invention provides for the use of fungi with altered morphology, which permits high-throughput screening and directed molecular evolution of expressed proteins. The same transformed fungi may be used (as shown in figure 1) to produce larger quantities of protein for isolation, characterization, and application testing, and may be suitable for commercial production of the protein as well.
Inventors:
EMALFARB MARK AARON (US)
PUNT PETER JAN
VAN ZEIJL CORNELIA
VAN DEN HONDEL CORNELIUS
PUNT PETER JAN
VAN ZEIJL CORNELIA
VAN DEN HONDEL CORNELIUS
Application Number:
PCT/US2001/012335
Publication Date:
February 27, 2003
Filing Date:
April 13, 2001
Export Citation:
Assignee:
EMALFARB MARK AARON (US)
International Classes:
C12N15/09; C12N1/00; C12N15/10; C12P21/02; C12Q1/04; C12R1/645; (IPC1-7): C12Q1/68; C12N1/14; C12N15/00; C07H21/02
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