Title:
HUMAN LIVER-LIKE THREE-DIMENSIONAL STRUCTURE, METHOD FOR EVALUATING HEPATOTOXICITY AND HUMAN LIVER-LIKE COMPLEX
Document Type and Number:
WIPO Patent Application WO/2020/171220
Kind Code:
A1
Abstract:
Long awaited is a human liver-like three-dimensional structure that makes it possible to carry out evaluation of human-specific toxicity and the like accurately and in a simple manner. The present invention provides a human liver-like three-dimensional structure comprising a heterospheroid, in which human hepatic cells and other human-derived cells which are not human hepatic cells are aggregated. This human liver-like three-dimensional structure is characterized in that the other human-derived cells are at least one selected from human hepatic stellate cells and the like, and the other human-derived cell to the human hepatic cell count ratio is at least 0.01 but less than 1.
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Inventors:
MAEKAWA TOSHIHIKO (JP)
NAGAO ERI (JP)
IDE IZUMI (JP)
KAJIYAMA SAKURA (JP)
NAGAO ERI (JP)
IDE IZUMI (JP)
KAJIYAMA SAKURA (JP)
Application Number:
PCT/JP2020/007156
Publication Date:
August 27, 2020
Filing Date:
February 21, 2020
Export Citation:
Assignee:
CYFUSE BIOMEDICAL KK (JP)
International Classes:
C12N5/071; C12N5/0775; C12N15/113; C12Q1/02; C12Q1/68; C12Q1/6837; C12Q1/686
Domestic Patent References:
| WO2017200111A1 | 2017-11-23 | |||
| WO2017067970A1 | 2017-04-27 |
Foreign References:
| JP2006504769A | 2006-02-09 |
Other References:
MOSEDALE, MERRIE ET AL.: "miR-122 release in exosomes precedes overt tolvaptan-induced necrosis in a primary human hepatocyte micropatterned coculture model", TOXICOLOGICAL SCIENCES, vol. 161, no. 1, 2018, pages 149 - 158, XP055734134
COSTA DE FREITAS, RENATA C. ET AL.: "Modulation of miR-26a-5p and miR-15b-5p exosomal expression associated with clopidogrel-induced hepatotoxicity in HepG2 cells", FRONTIERS IN PHARMACOLOGY, vol. 8, 2017, pages 906/1 - 906/11, XP055734135
SHIMIZU, ICHIRO: "Sho-saiko-to for protection against hepatic fibrosis and carcinoma", JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, ORIENTAL MEDICINE FROM STANDPOINT OF MODERN WESTERN MEDICINE, 2003, XP008093629
NISHITO, YUKINA: "Tetrandrine, an anti- fibrotic substance, regulates autophagic pathway in hepatic stellate cells", LECTURE ABSTRACTS OF THE 2014 CONFERENCE OF JAPAN SOCIETY OF BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, 2014
COSTA DE FREITAS, RENATA C. ET AL.: "Modulation of miR-26a-5p and miR-15b-5p exosomal expression associated with clopidogrel-induced hepatotoxicity in HepG2 cells", FRONTIERS IN PHARMACOLOGY, vol. 8, 2017, pages 906/1 - 906/11, XP055734135
SHIMIZU, ICHIRO: "Sho-saiko-to for protection against hepatic fibrosis and carcinoma", JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, ORIENTAL MEDICINE FROM STANDPOINT OF MODERN WESTERN MEDICINE, 2003, XP008093629
NISHITO, YUKINA: "Tetrandrine, an anti- fibrotic substance, regulates autophagic pathway in hepatic stellate cells", LECTURE ABSTRACTS OF THE 2014 CONFERENCE OF JAPAN SOCIETY OF BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, 2014
Attorney, Agent or Firm:
KOBAYASHI Hiroshi et al. (JP)
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