Title:
HYALURONIC ACID HYDROLAZE AND ENCODING SEQUENCE THEREOF, AND METHOD FOR PREPARING OLIGOMERIC HYALURONATE BY MEANS OF USING SAME
Document Type and Number:
WIPO Patent Application WO/2021/129353
Kind Code:
A1
Abstract:
Disclosed are a hyaluronic acid hydrolaze and an encoding sequence thereof, and a method for preparing an oligomeric hyaluronate by means of using same. The nucleotide sequence encoding the hyaluronic acid hydrolaze can be transferred into engineering bacteria through methods of transduction, transformation and conjugal transfer, and the hyaluronic acid hydrolaze is efficiently expressed by means of regulating the expression of the encoding gene, which provides an effective way to produce oligomeric hyaluronic acids. The use of the hyaluronic acid hydrolaze to hydrolyze hyaluronic acids has the advantages of simple operation, mild conditions and a higher efficiency, and the resulting oligomeric hyaluronic acids have a relatively high purity; in addition, hyaluronic acid hydrolazes from a fermentation source are low in cost, and are therefore also suitable for large-scale industrial production.
Inventors:
LI BIN (CN)
YU YUNDONG (CN)
XU FEI (CN)
ZHU JUN (CN)
SHAO FANTAO (CN)
YU YUNDONG (CN)
XU FEI (CN)
ZHU JUN (CN)
SHAO FANTAO (CN)
Application Number:
PCT/CN2020/133978
Publication Date:
July 01, 2021
Filing Date:
December 04, 2020
Export Citation:
Assignee:
JIANGSU CHENGXIN PHARMACEUTICAL CO LTD (CN)
International Classes:
C12N1/19; C12N9/26; C12N1/21; C12N15/56; C12N15/70; C12N15/75; C12N15/81; C12P19/04; C12P19/14
Foreign References:
| CN110951714A | 2020-04-03 | |||
| CN102690847A | 2012-09-26 | |||
| CN106367459A | 2017-02-01 | |||
| CN103484513A | 2014-01-01 |
Other References:
DATABASE PROTEIN 28 April 2020 (2020-04-28), ANONYMOUS: "Ig-like domain-containing protein [Lederbergia lentus]", XP055823925, retrieved from NCBI Database accession no. WP_066143691
DATABASE PROTEIN 24 April 2014 (2014-04-24), ANONYMOUS: "hyaluronidase, partial [Bacillus sp. A50]", XP055823927, retrieved from NCBI Database accession no. AHB61202
GUO XUEPING, SHI YANLI, SHENG JUZHENG, WANG FENGSHAN: "A Novel Hyaluronidase Produced by Bacillus sp. A50", PLOS ONE, vol. 9, no. 4, 15 April 2014 (2014-04-15), XP055823932, DOI: 10.1371/journal.pone.0094156
DATABASE PROTEIN 24 April 2014 (2014-04-24), ANONYMOUS: "hyaluronidase, partial [Bacillus sp. A50]", XP055823927, retrieved from NCBI Database accession no. AHB61202
GUO XUEPING, SHI YANLI, SHENG JUZHENG, WANG FENGSHAN: "A Novel Hyaluronidase Produced by Bacillus sp. A50", PLOS ONE, vol. 9, no. 4, 15 April 2014 (2014-04-15), XP055823932, DOI: 10.1371/journal.pone.0094156
Attorney, Agent or Firm:
BEYOND ATTORNEYS AT LAW (CN)
Download PDF:
Previous Patent: UNMANNED AERIAL VEHICLE BASE STATION
Next Patent: DATA INDEX MANAGEMENT METHOD AND DEVICE IN STORAGE SYSTEM
Next Patent: DATA INDEX MANAGEMENT METHOD AND DEVICE IN STORAGE SYSTEM