Title:
HYDROGENOPHILUS GENUS BACTERIUM TRANSFORMANT
Document Type and Number:
WIPO Patent Application WO/2019/207812
Kind Code:
A1
Abstract:
This transformant obtained by introducing, to hydrogenophilus genus bacteria, DNA of (a1), (a2) or (a3), and (b) a gene of alcohol dehydrogenase, can efficiently produce isobutanol using carbon dioxide as the only source of carbon. DNA (a1) comprises the base sequence represented by SEQ ID No: 1. DNA (a2) comprises a base sequence having at least 90% identity with SEQ ID No: 1, and encodes a polypeptide that has a 2-ketoacid decarboxylase activity. DNA (a3) hybridizes with a DNA comprising a base sequence complimentary to SEQ ID No: 1 under a stringent condition, and encodes a polypeptide that has a 2-ketoacid decarboxylase activity.
Inventors:
YUKAWA HIDEAKI (JP)
OHTANI NAOTO (JP)
ISHII MASAHARU (JP)
OHTANI NAOTO (JP)
ISHII MASAHARU (JP)
Application Number:
PCT/JP2018/024073
Publication Date:
October 31, 2019
Filing Date:
June 25, 2018
Export Citation:
Assignee:
UTILIZATION OF CARBON DIOXIDE INST CO LTD (JP)
UNIV TOKYO (JP)
UNIV TOKYO (JP)
International Classes:
C12N15/60; C12N1/21; C12N9/04; C12N15/53; C12P7/06; C12P7/16; C12P13/06
Domestic Patent References:
| WO2010113832A1 | 2010-10-07 | |||
| WO2001096573A1 | 2001-12-20 | |||
| WO2010113832A1 | 2010-10-07 | |||
| WO2001096573A1 | 2001-12-20 |
Foreign References:
| US20140273128A1 | 2014-09-18 | |||
| US20110287499A1 | 2011-11-24 | |||
| JPH06277082A | 1994-10-04 | |||
| JP277082A |
Other References:
DATABASE GenBank [online] 18 May 2013 (2013-05-18), "MULTISPECIES: alanine dehydrogenase [Geobacillus]", XP055646041, Database accession no. WP_013146672
ATSUMI SHANAI TLIAO JC., NATURE, vol. 451, 2008, pages 86 - 89
ATSUMI SWU TYECKL EMHAWKINS SDBUELTER TLIAO JC: "Engineering the isobutanol biosynthetic pathway in Escherichia coli by comparison of three aldehyde reductase/alcohol dehydrogenase genes", APPL. MICROBIOL. BIOTECHNOL., vol. 85, 2010, pages 651 - 657, XP002765103, DOI: 10.1007/s00253-009-2085-6
LI SWEN JJIA X: "Engineering Bacillus subtilis for isobutanol production by heterologous Ehrlich pathway construction and the biosynthetic 2-ketoisovalerate precursor pathway overexpression.", APPL. MICROBIOL. BIOTECHNOL., vol. 91, 2011, pages 577 - 589
JEON JMPARK HSEO HM, KIM JHBHATIA SKSATHIYANARAYANAN GSONG HSPARK SHCHOI KYSANG BIYANG YH: "Isobutanol production from an engineered Shewanella oneidensis MR-1", BIOPROCESS BIOSYST. ENG., vol. 38, 2015, pages 2147 - 2154
ATSUMI SHIGASHIDE WLIAO JC: "Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde", NAT. BIOTECHNOL., vol. 27, 2009, pages 1177 - 1180, XP008153202, DOI: 10.1038/nbt.1586
LU JBRIGHAM CJGAI CSSINSKEY AJ: "Studies on the production of branched-chain alcohols in engineered Ralstonia eutropha.", APPL. MICROBIOL. BIOTECHNOL., vol. 96, 2012, pages 283 - 297
LIN PPRABE KSTAKASUMI JLKADISCH MARNOLD FHLIAO JC: "Isobutanol production at elevated temperatures in thermophilic Geobacillus thermoglucosidasius.", METAB. ENG., vol. 24, 2014, pages 1 - 8
LIU SDIEN BSNICHOLS NNBISCHOFF KMHUGHES SRCOTTA MA: "Coexpression of pyruvate decarboxylase and alcohol dehydrogenase genes in Lactobacillus brevis", FEMS MICROBIOL. LETT., vol. 274, 2007, pages 291 - 297
TALARICO LAGIL MAYOMANO LPINGRAM LOMAUPIN-FURLOW JA: "Construction and expression of an ethanol production operon in Gram-positive bacteria", MICROBIOLOGY, vol. 151, 2005, pages 4023 - 4031
DENG MDCOLEMAN JR: "Ethanol synthesis by genetic engineering in cyanobacteria", APPL. ENVIRON. MICROBIOL., vol. 65, 1999, pages 523 - 528, XP003016237
LUAN GQI YWANG MLI ZDUAN YTAN XLU X: "Combinatory strategy for characterizing and understanding the ethanol synthesis pathway in cyanobacteria cell factories", BIOTECHNOL. BIOFUELS, vol. 8, 2015, pages 184
KELLER MWLIPSCOMB GLNGUYEN DMCROWLEY ATSCHUT GJSCOTT IKELLY RMADAMS MWW: "Ethanol production by the hyperthermophilic archaeon Pyrococcus furiosus by expression of bacterial bifunctional alcohol dehydrogenases", MICROB. BIOTECHNOL., vol. 10, 2017, pages 1535 - 1545
CHUNG DCHA MSNYDER ENELKINS JGGUSS AMWESTPHELING: "Cellulosic ethanol production via consolidated bioprocessing at 75°C by engineered Caldicellulosiruptor bescii.", J.BIOTECHNOL. BIOFUELS, vol. 8, 2015, pages 163, XP021229427, DOI: 10.1186/s13068-015-0346-4
WADA MNARITA KYOKOTA A: "Alanine production in an H+-ATPase-and lactate dehydrogenase-defective mutant of Escherichia coli expressing alanine dehydrogenase", APPL. MICROBIOL. BIOTECHNOL., vol. 76, 2007, pages 819 - 825, XP019538726, DOI: 10.1007/s00253-007-1065-y
LEE MSMITH GMEITEMAN MAALTMAN E: "Aerobic production of alanine by Escherichia coli aceF ldhA mutants expressing the Bacillus sphaericus alaD gene", APPL. MICROBIOL. BIOTECHNOL., vol. 65, 2004, pages 56 - 60, XP055034646, DOI: 10.1007/s00253-004-1560-3
UHLENBUSCH ISAHM HSPRENGER GA: "Expression of an L-alanine dehydrogenase gene in Zymomonas mobilis and excretion of L-alanine", APPL. ENVIRON. MICROBIOL., vol. 57, 1991, pages 1360 - 1366, XP002640779
HOLS PKLEEREBEZEM MSCHANCK ANFERAIN THUGENHOLTZ JDELCOUR JDE VOS WM.: "Conversion of Lactococcus lactis from homolactic to homoalanine fermentation through metabolic engineering", NAT. BIOTECHNOL., vol. 17, 1999, pages 588 - 592, XP002225554, DOI: 10.1038/9902
JOJIMA TFUJII MMORI EINUI MYUKAWA H: "Engineering of sugar metabolism of Corynebacterium glutamicum for production of amino acid L-alanine under oxygen deprivation", APPL. MICROBIOL. BIOTECHNOL., vol. 87, 2010, pages 159 - 165, XP019841516
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, vol. 41, 1977, pages 685 - 690
"NCBI", Database accession no. NC_001384.1
"GenBank", Database accession no. FJ437239.1
GENE, vol. 70, 1998, pages 191 - 197
GENE, vol. 145, 1994, pages 69 - 73
JOURNAL OF MOLECULAR BIOLOGY, vol. 53, 1970, pages 159 - 162
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 123, 2017, pages 540 - 546
See also references of EP 3786299A4
ATSUMI SHANAI TLIAO JC., NATURE, vol. 451, 2008, pages 86 - 89
ATSUMI SWU TYECKL EMHAWKINS SDBUELTER TLIAO JC: "Engineering the isobutanol biosynthetic pathway in Escherichia coli by comparison of three aldehyde reductase/alcohol dehydrogenase genes", APPL. MICROBIOL. BIOTECHNOL., vol. 85, 2010, pages 651 - 657, XP002765103, DOI: 10.1007/s00253-009-2085-6
LI SWEN JJIA X: "Engineering Bacillus subtilis for isobutanol production by heterologous Ehrlich pathway construction and the biosynthetic 2-ketoisovalerate precursor pathway overexpression.", APPL. MICROBIOL. BIOTECHNOL., vol. 91, 2011, pages 577 - 589
JEON JMPARK HSEO HM, KIM JHBHATIA SKSATHIYANARAYANAN GSONG HSPARK SHCHOI KYSANG BIYANG YH: "Isobutanol production from an engineered Shewanella oneidensis MR-1", BIOPROCESS BIOSYST. ENG., vol. 38, 2015, pages 2147 - 2154
ATSUMI SHIGASHIDE WLIAO JC: "Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde", NAT. BIOTECHNOL., vol. 27, 2009, pages 1177 - 1180, XP008153202, DOI: 10.1038/nbt.1586
LU JBRIGHAM CJGAI CSSINSKEY AJ: "Studies on the production of branched-chain alcohols in engineered Ralstonia eutropha.", APPL. MICROBIOL. BIOTECHNOL., vol. 96, 2012, pages 283 - 297
LIN PPRABE KSTAKASUMI JLKADISCH MARNOLD FHLIAO JC: "Isobutanol production at elevated temperatures in thermophilic Geobacillus thermoglucosidasius.", METAB. ENG., vol. 24, 2014, pages 1 - 8
LIU SDIEN BSNICHOLS NNBISCHOFF KMHUGHES SRCOTTA MA: "Coexpression of pyruvate decarboxylase and alcohol dehydrogenase genes in Lactobacillus brevis", FEMS MICROBIOL. LETT., vol. 274, 2007, pages 291 - 297
TALARICO LAGIL MAYOMANO LPINGRAM LOMAUPIN-FURLOW JA: "Construction and expression of an ethanol production operon in Gram-positive bacteria", MICROBIOLOGY, vol. 151, 2005, pages 4023 - 4031
DENG MDCOLEMAN JR: "Ethanol synthesis by genetic engineering in cyanobacteria", APPL. ENVIRON. MICROBIOL., vol. 65, 1999, pages 523 - 528, XP003016237
LUAN GQI YWANG MLI ZDUAN YTAN XLU X: "Combinatory strategy for characterizing and understanding the ethanol synthesis pathway in cyanobacteria cell factories", BIOTECHNOL. BIOFUELS, vol. 8, 2015, pages 184
KELLER MWLIPSCOMB GLNGUYEN DMCROWLEY ATSCHUT GJSCOTT IKELLY RMADAMS MWW: "Ethanol production by the hyperthermophilic archaeon Pyrococcus furiosus by expression of bacterial bifunctional alcohol dehydrogenases", MICROB. BIOTECHNOL., vol. 10, 2017, pages 1535 - 1545
CHUNG DCHA MSNYDER ENELKINS JGGUSS AMWESTPHELING: "Cellulosic ethanol production via consolidated bioprocessing at 75°C by engineered Caldicellulosiruptor bescii.", J.BIOTECHNOL. BIOFUELS, vol. 8, 2015, pages 163, XP021229427, DOI: 10.1186/s13068-015-0346-4
WADA MNARITA KYOKOTA A: "Alanine production in an H+-ATPase-and lactate dehydrogenase-defective mutant of Escherichia coli expressing alanine dehydrogenase", APPL. MICROBIOL. BIOTECHNOL., vol. 76, 2007, pages 819 - 825, XP019538726, DOI: 10.1007/s00253-007-1065-y
LEE MSMITH GMEITEMAN MAALTMAN E: "Aerobic production of alanine by Escherichia coli aceF ldhA mutants expressing the Bacillus sphaericus alaD gene", APPL. MICROBIOL. BIOTECHNOL., vol. 65, 2004, pages 56 - 60, XP055034646, DOI: 10.1007/s00253-004-1560-3
UHLENBUSCH ISAHM HSPRENGER GA: "Expression of an L-alanine dehydrogenase gene in Zymomonas mobilis and excretion of L-alanine", APPL. ENVIRON. MICROBIOL., vol. 57, 1991, pages 1360 - 1366, XP002640779
HOLS PKLEEREBEZEM MSCHANCK ANFERAIN THUGENHOLTZ JDELCOUR JDE VOS WM.: "Conversion of Lactococcus lactis from homolactic to homoalanine fermentation through metabolic engineering", NAT. BIOTECHNOL., vol. 17, 1999, pages 588 - 592, XP002225554, DOI: 10.1038/9902
JOJIMA TFUJII MMORI EINUI MYUKAWA H: "Engineering of sugar metabolism of Corynebacterium glutamicum for production of amino acid L-alanine under oxygen deprivation", APPL. MICROBIOL. BIOTECHNOL., vol. 87, 2010, pages 159 - 165, XP019841516
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, vol. 41, 1977, pages 685 - 690
"NCBI", Database accession no. NC_001384.1
"GenBank", Database accession no. FJ437239.1
GENE, vol. 70, 1998, pages 191 - 197
GENE, vol. 145, 1994, pages 69 - 73
JOURNAL OF MOLECULAR BIOLOGY, vol. 53, 1970, pages 159 - 162
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 123, 2017, pages 540 - 546
See also references of EP 3786299A4
Attorney, Agent or Firm:
TADA Eiko et al. (JP)
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