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Title:
IDENTIFICATION METHOD FOR INDIVIDUAL BROCCOLI PLANTS HAVING CLUBROOT RESISTANCE, BREEDING METHOD FOR CLUBROOT-RESISTANT BROCCOLI
Document Type and Number:
WIPO Patent Application WO/2016/010068
Kind Code:
A1
Abstract:
A method that identifies individual broccoli plants that have resistance to clubroot by identifying individuals that have a DNA sequence polymorphism. The method detects DNA that is described by (1) and (2). (1) DNA that includes an SSR in a base sequence described by one or more of SEQ ID NO:1, 2, 4, 5, and 6 or a portion of said DNA. (2) DNA that includes an SNP at position 61 of the base sequence described by SEQ ID NO:3 or a portion of said DNA.

Inventors:
MIKAMI MAKI (JP)
MIKAMI TAKAHARU (JP)
Application Number:
PCT/JP2015/070249
Publication Date:
January 21, 2016
Filing Date:
July 15, 2015
Export Citation:
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Assignee:
MIKADO KYOWA SEED CO LTD (JP)
International Classes:
C12Q1/68; A01H5/00; C12N15/09
Foreign References:
JP2004135554A2004-05-13
Other References:
SEIYA TSUSHIMA: "Perspective and Problem of Integrated Pest Management for Plant Pathology -A Case Study: Clubroot Disease of Crucifers", ANNUAL REPORT OF THE KANTO-TOSAN PLANT PROTECTION SOCIETY, vol. 52, 2005, pages 1 - 8
NAGAOKA, T. ET AL.: "Identification of QTLs that control clubroot resistance in Brassica oleracea and comparative analysis of clubroot resistance genes between B. rapa and B. oleracea", THEORETICAL AND APPLIED GENETICS, vol. 120, 2010, pages 1335 - 1346, XP019797011
ROCHERIEUX, J. ET AL.: "Isolate-specific and broad-spectrum QTLs are involved in the control of clubroot in Brassica oleracea", THEORETICAL AND APPLIED GENETICS, vol. 108, 2004, pages 1555 - 1563, XP002633433, DOI: doi:10.1007/s00122-003-1580-x
ZHONGYUN, P. ET AL.: "Genetics of Clubroot Resistance in Brassica Species", JOURNAL OF PLANT GROWTH REGULATION, vol. 28, 2009, pages 252 - 264, XP019739174, DOI: doi:10.1007/s00344-009-9093-8
WERNER, S. ET AL.: "Genetic mapping of clubroot resistance genes in oilseed rape", THEORETICAL AND APPLIED GENETICS, vol. 116, 2008, pages 363 - 372, XP002633434, DOI: doi:10.1007/s00122-007-0674-2
HATAKEYAMA, K. ET AL.: "Identification and Characterization of Crrla, a Gene for Resistance to Clubroot Disease (Plasmodiophora brassicae Woronin) in Brassica rapa L.", PLOS ONE, vol. 8, no. 1): e54745., 30 January 2013 (2013-01-30), pages 1 - 10
Attorney, Agent or Firm:
KOBAYASHI, Masaharu et al. (JP)
Shoji Kobayashi (JP)
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