Title:
IMMOBILIZED PROTEIN AND PROCESS FOR PRODUCING THE SAME
Document Type and Number:
WIPO Patent Application WO/2004/089977
Kind Code:
A1
Abstract:
By introducing a polymer compound having a primary amino group in its repeating structure onto the surface of a support, the content of the primary amino group usable in immobilization is increased and thus a protein is immobilized at a high density at one site in the carboxyl end.
Inventors:
IWAKURA MASAHIRO (JP)
HIROTA KIYONORI (JP)
HIROTA KIYONORI (JP)
Application Number:
PCT/JP2004/005160
Publication Date:
October 21, 2004
Filing Date:
April 09, 2004
Export Citation:
Assignee:
NAT INST OF ADVANCED IND SCIEN (JP)
IWAKURA MASAHIRO (JP)
HIROTA KIYONORI (JP)
IWAKURA MASAHIRO (JP)
HIROTA KIYONORI (JP)
International Classes:
C12N15/09; C07K17/00; C07K17/08; C12N11/06; (IPC1-7): C07K7/06; C12N15/00
Foreign References:
| JP3047020B1 | 2000-05-29 | |||
| JPH08503066A | 1996-04-02 | |||
| JPS6274286A | 1987-04-06 | |||
| JPH1045798A | 1998-02-17 | |||
| JP2003344396A | 2003-12-03 |
Other References:
UNG-JIN KIM ET AL.: "Polyallylamine-grafted cellulose gel as high-capacity anion-exchanger", J. CHROMATOGRAPHY A, vol. 946, 2002, pages 283 - 289, XP004333389
TAKENAWA T. ET AL.: "Cyanocysteine-mediated molecular dissection of dihydrofolate reductase: Occurrence of intra- and inter-molecular reactions forming a peptide bond", J. BIOCHEM., vol. 123, no. 6, 1998, pages 1137 - 1144, XP009018697
TAKENAWA T. ET AL.: "Cyanocysteine-mediated molecular dissection of dihydrofolate reductase: Occurrence of intra- and inter-molecular reactions forming a peptide bond", J. BIOCHEM., vol. 123, no. 6, 1998, pages 1137 - 1144, XP009018697
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