Title:
IMMUNE INDUCER CONTAINING POLYNUCLEOTIDE-PEPTIDE CONJUGATE AND PHARMACEUTICAL COMPOSITION CONTAINING SAME
Document Type and Number:
WIPO Patent Application WO/2021/193900
Kind Code:
A1
Abstract:
The present invention provides an immune inducer containing a polynucleotide-peptide conjugate or a pharmaceutically acceptable salt thereof as an active ingredient. The polynucleotide-peptide conjugate comprises a single-stranded polynucleotide or polynucleotide derivative including CpG motifs, a peptide, and a spacer covalently bonded to the polynucleotide or polynucleotide derivative at one end and covalently bonded to the peptide at the other end. In the peptide, one or more contiguous amino acids at the N terminal of an MHC binding peptide are replaced by amino acids having a reactive functional group for covalently binding to the spacer. The one or more contiguous amino acids do not include an anchor residue for forming an MHC bond.
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Inventors:
MOCHIZUKI SHINICHI (JP)
KOIZUMI MAKOTO (JP)
MORITA KOJI (JP)
KOIZUMI MAKOTO (JP)
MORITA KOJI (JP)
Application Number:
PCT/JP2021/012787
Publication Date:
September 30, 2021
Filing Date:
March 26, 2021
Export Citation:
Assignee:
THE UNIV OF KITAKYUSHU (JP)
DAIICHI SANKYO CO LTD (JP)
DAIICHI SANKYO CO LTD (JP)
International Classes:
A61K47/65; A61K31/7088; A61K39/39; A61K47/64; A61K48/00; A61P35/00; A61P37/04; C07K7/06; C12N15/117
Domestic Patent References:
| WO2012147805A1 | 2012-11-01 | |||
| WO2017057540A1 | 2017-04-06 | |||
| WO2015118789A1 | 2015-08-13 | |||
| WO2014157704A1 | 2014-10-02 |
Foreign References:
| JP2017500313A | 2017-01-05 | |||
| JP2008509072A | 2008-03-27 | |||
| JP2019038090W | 2019-09-27 |
Other References:
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H. WAGNER: "Bacterial CpG DNA Activates Immune Cells to Signal Infectious Danger", ADV. IMMUNOL., vol. 73, 1999, pages 329 - 368
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S. YAMAMOTOT. YAMAMOTOT. TOKUNAGA: "The discovery of immunostimulatory DNA sequence", SPRINGER SEMINARS IN IMMUNOPATHOLOGY, vol. 22, 2000, pages 11 - 19
KHAN S. ET AL.: "Distinct Uptake Mechanisms but Similar Intracellular Processing of Two Different Toll-like Receptor Ligand-Peptide Conjugates in Dendritic Cells", J. BIOL. CHEM., vol. 282, 2007, pages 21145 - 21159, XP055613005, DOI: 10.1074/jbc.M701705200
KRAMER K. ET AL.: "Intracellular Cleavable CpG Oligodeoxynucleotide-Antigen Conjugate Enhances Anti-tumor Immunity", MOL. THER., vol. 25, 2017, pages 62 - 70, XP055613007, DOI: 10.1016/j.ymthe.2016.10.001
KRAMER K. ET AL.: "Comparative Study of 5'- and 3'-Linked CpG-Antigen Conjugates for the Induction of Cellular Immune Responses", ACS OMEGA, vol. 2, no. 1, 2017, pages 227 - 235, XP055617506, DOI: 10.1021/acsomega.6b00368
SHIROTA H.KLINMAN D. M.: "TLR-9 Agonist Immunostimulatory Sequence Adjuvants Linked to Cancer Antigens", METHODS MOL. BIOL., vol. 1139, 2014, pages 337 - 344, XP055700123, DOI: 10.1007/978-1-4939-0345-0_27
IMMUNOGENETICS, vol. 50, 1999, pages 213 - 219
IMMUNOGENETICS, vol. 41, 1995, pages 178 - 228
"GenBank", Database accession no. AAC60634.1
IMMUNOGENETICS, vol. 60, 2008, pages 759 - 765
FRONT. IMMUNOL., vol. 6, 2015, pages 377
J. IMMUNOL., vol. 174, no. 8, 2005, pages 4812 - 4820
CAS, no. 2351397-00-5
CAS , no. 29768-80-7
NUCLEIC ACIDS RESEARCH, vol. 12, 1984, pages 4539
BIOORG. MED. CHEM., vol. 16, pages 941 - 949
PROC. NATL. ACAD. SCI. USA., vol. 102, no. 47, 2005, pages 17107 - 12
RAPIN, NICOLAS ET AL.: "MHC motif viewer", IMMUNOGENETICS, vol. 60, no. 12, 2008, pages 759 - 765, XP019654076, DOI: 10.1007/s00251-008-0330-2
H. WAGNER: "Bacterial CpG DNA Activates Immune Cells to Signal Infectious Danger", ADV. IMMUNOL., vol. 73, 1999, pages 329 - 368
M. KRIEG: "CpG Motifs in Bacterial DNA and Their Immune Effects", ANNU. REV. IMMUNOL., vol. 20, 2002, pages 709 - 760, XP009016455, DOI: 10.1146/annurev.immunol.20.100301.064842
S. YAMAMOTOT. YAMAMOTOT. TOKUNAGA: "The discovery of immunostimulatory DNA sequence", SPRINGER SEMINARS IN IMMUNOPATHOLOGY, vol. 22, 2000, pages 11 - 19
KHAN S. ET AL.: "Distinct Uptake Mechanisms but Similar Intracellular Processing of Two Different Toll-like Receptor Ligand-Peptide Conjugates in Dendritic Cells", J. BIOL. CHEM., vol. 282, 2007, pages 21145 - 21159, XP055613005, DOI: 10.1074/jbc.M701705200
KRAMER K. ET AL.: "Intracellular Cleavable CpG Oligodeoxynucleotide-Antigen Conjugate Enhances Anti-tumor Immunity", MOL. THER., vol. 25, 2017, pages 62 - 70, XP055613007, DOI: 10.1016/j.ymthe.2016.10.001
KRAMER K. ET AL.: "Comparative Study of 5'- and 3'-Linked CpG-Antigen Conjugates for the Induction of Cellular Immune Responses", ACS OMEGA, vol. 2, no. 1, 2017, pages 227 - 235, XP055617506, DOI: 10.1021/acsomega.6b00368
SHIROTA H.KLINMAN D. M.: "TLR-9 Agonist Immunostimulatory Sequence Adjuvants Linked to Cancer Antigens", METHODS MOL. BIOL., vol. 1139, 2014, pages 337 - 344, XP055700123, DOI: 10.1007/978-1-4939-0345-0_27
IMMUNOGENETICS, vol. 50, 1999, pages 213 - 219
IMMUNOGENETICS, vol. 41, 1995, pages 178 - 228
"GenBank", Database accession no. AAC60634.1
IMMUNOGENETICS, vol. 60, 2008, pages 759 - 765
FRONT. IMMUNOL., vol. 6, 2015, pages 377
J. IMMUNOL., vol. 174, no. 8, 2005, pages 4812 - 4820
CAS, no. 2351397-00-5
CAS , no. 29768-80-7
NUCLEIC ACIDS RESEARCH, vol. 12, 1984, pages 4539
BIOORG. MED. CHEM., vol. 16, pages 941 - 949
PROC. NATL. ACAD. SCI. USA., vol. 102, no. 47, 2005, pages 17107 - 12
Attorney, Agent or Firm:
YAMAMOTO, Osamu et al. (JP)
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