Title:
IMMUNOCHROMATOGRAPHIC TEST PIECE FOR EXTRACTING AND MEASURING CARBOHYDRATE ANTIGEN, COMPRISING HYDROPHOBIC MATERIAL IMPREGNATED WITH NITRITE OR SOLID ACIDIC REAGENT AND THUS CAPABLE OF CONTROLLING DEVELOPMENT OF SPECIMEN
Document Type and Number:
WIPO Patent Application WO/2020/158765
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide an immunochromatographic test piece for controlling the development of a specimen on the immunochromatographic test piece so as to appropriately control a treatment with an acidic reagent, a nitrite and a neutralizing reagent, and an immunochromatographic method in which the test piece is used. An immunochromatographic test piece for extracting and measuring a carbohydrate antigen in a specimen, said immunochromatographic test piece comprising: a sample pad to which the specimen mixed with a nitrite or an acidic solution is added; a label region containing a labeled antibody which is prepared by labeling an antibody to the carbohydrate antigen; and a detection region in which the antibody for the carbohydrate antigen is immobilized, wherein an antibody–carbohydrate antigen–labeled antibody complex is formed in the detection region to thereby measure the carbohydrate antigen. The immunochromatographic test piece, which has a region impregnated with a neutralizing reagent upstream of the label region and, further upstream of the region impregnated with the neutralizing reagent, a region impregnated with a solid acidic reagent in the case of using the specimen as a mixture with the nitrite, or a region impregnated with a nitrite in the case of using the specimen as a mixture with the acidic solution, is characterized in that a hydrophobic material is used in the region impregnated with the nitrite or the solid acidic reagent.
Inventors:
KATO DAISUKE (JP)
MURAMATSU SHINO (JP)
MURAMATSU SHINO (JP)
Application Number:
PCT/JP2020/003066
Publication Date:
August 06, 2020
Filing Date:
January 29, 2020
Export Citation:
Assignee:
DENKA SEIKEN KK (JP)
International Classes:
G01N33/53; G01N33/543; G01N33/569
Domestic Patent References:
| WO2005121794A1 | 2005-12-22 |
Foreign References:
| JP2018151330A | 2018-09-27 | |||
| JP2018151331A | 2018-09-27 | |||
| JP2018151329A | 2018-09-27 | |||
| JP2014232064A | 2014-12-11 | |||
| JP2018151329A | 2018-09-27 | |||
| JP2018151330A | 2018-09-27 | |||
| JP2018151331A | 2018-09-27 | |||
| JP2019012974A | 2019-01-24 |
Other References:
See also references of EP 3919904A4
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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