CROSS-REFERENCE TO RELATED APPLICATION

This application claims priority to U.S. Provisional Application No. 61/845,747 filed on July 12, 2013, herein incorporated by reference.

ACKNOWLEDGMENT OF GOVERNMENT SUPPORT

This invention was made with government support under AI051334 awarded by the National Institute of Allergy and Infectious Diseases. The government has certain rights in the invention.

FIELD

The application relates to fragments of the Tp0751 protein (also known as pallilysin) and methods of their use to stimulate an immune response, for example use as a vaccine in a mammal. In some examples, such stimulation of the immune response protects the mammal from subsequent Treponema pallidum subsp. pallidum infection. Also provided are Tp0751 protein fragments, nucleic acids encoding such fragments, as well as compositions including such.

BACKGROUND

Syphilis is a chronic infection caused by the spirochete Treponema pallidum subsp. pallidum that is generally transmitted either through sexual contact or vertically from an infected mother to her fetus. Syphilis is a global health concern with an estimated burden of 25 million people worldwide and an estimated annual new incidence rate of 12 million cases. Further, infection with T. pallidum has been shown to increase the likelihood of contracting HIV.

Congenital syphilis is recognized as the most significant disease affecting pregnancies and newborns worldwide, with over 2 million pregnant women estimated to be infected with syphilis every year. Without treatment, there are adverse outcomes in 69% of cases, including spontaneous abortion or stillbirth, neonatal complications, and infant mortality.

As a multi-stage disease, syphilis has historically been called the great imitator due to the similarity of its symptoms to other diseases. Primary syphilis is characterized by a painless open sore called a chancre, which develops on average 3 weeks after infection at the site of inoculation. The chancre spontaneously resolves, and 1-3 months later secondary symptoms may present. Secondary infection typically manifests as a generalized rash, often localizing to the trunk of the body, palms of the hands, and soles of the feet. After 1-3 months, secondary symptoms resolve and the disease enters an asymptomatic latent phase. In some instances the disease can progress from latency to a tertiary stage, which can involve the development of gummas, central nervous system complications or cardiovascular disease. Since the symptoms of syphilis infection are so similar to other diseases, and resolve on their own, syphilis has always been a challenging disease to diagnose clinically.

During primary infection when a chancre is present, dark-field microscopy and/or PCR can be performed to identify spirochetes present at the site of infection. The chancre normally resolves in 4-6 weeks, and often goes unnoticed if internally located in either the anus or vagina, making diagnosis by dark-field microscopy extremely limited.

Currently, there is no vaccine available to provide protection from T. pallidum. Thus, there is a need for therapies that can prevent or reduce the undesirable consequences of T.

pallidum infection.

SUMMARY

It is shown herein that 2/3 rabbits vaccinated with a fragment of Tp0751 (amino acids 24-237) and then challenged with T. pallidum had complete prevention of dissemination to the popliteal lymph nodes. 100% of the unvaccinated rabbits had unequivocal bacterial

dissemination to the popliteal lymph nodes as demonstrated by the gold standard rabbit infectivity test. In addition, chancres from immunized animals were smaller, less ulcerated, and had fewer bacteria than control animals. Although the rabbits still had an inflammatory reaction to T. pallidum, the antibodies to Tp0751 generated following immunization were found to protect against certain types of damage {e.g., ulceration, necrosis).

Thus provided herein are methods of simulating an immune response in a subject, such as a mammal, by administering to the subject one or more native or non-native fragments of Tp0751 (such as any of SEQ ID NOS: 3-10), or one or more fragments of a Tp0751 peptide that is not naturally occurring, such as a Tp0751 peptide that has been modified. Examples of modifications that can be made to a Tp0751 peptide (such as to any of SEQ ID NOS: 3-10), include, mutations {e.g., a Tp0751 which includes a mutated active site, e.g., SEQ ID NOS: 4-6 and 8-10), modification with polyethylene glycol or methoxy polyethylene glycol (PEGylation, such as for example to increase the half-life of the protein) and direct or indirect {e.g., via a linker) fusion to another peptide (such as diphtheria toxin, tetanus toxin, keyhole limpet hemocyanin (KLH), a histidine tag {e.g., 6-His). In some examples, the immune response is a B cell response. Methods for inducing serum antibodies which have neutralizing activity for T. pallidum are provided, for example which include administering to the subject one or more fragments of Tp0751 (such as any of SEQ ID NOS: 3-10) or a modified Tp0751 peptide.

Methods for vaccinating a human against T. pallidum infection are provided, for example which include administering to the subject one or more fragments of Tp0751 (such as any of SEQ ID NOS: 3-10) or a modified Tp0751 peptide.

Such methods can be used to prevent, treat or reduce the severity of infections caused by T. pallidum. In some examples, such administration protects the subject from subsequent infection by T. pallidum, such as a reduction in one or more consequences of T. pallidum infection, such as reducing one or more of chancre volume, % of chancers that ulcerate, dissemination of T. pallidum to lymph nodes, and the like. Such a reduction does not require 100% reduction, for example, reductions of at least 10%, at least 20%, at least 25%, at least 50%, at least 75%, at least 80%, or at least 90% can be sufficient.

Also provided are Tp0751 protein fragments {e.g., SEQ ID NOS: 3 and 7, which can include an N- or C-terminal tag) as well as fragments which include a mutated active site {e.g., SEQ ID NOS: 4-6 and 8-10), variants thereof, and nucleic acid molecules encoding such proteins (such as a cDNA molecule). In one example, a Tp0751 protein fragment (such as any of SEQ ID NOS: 3-10) is modified with polyethylene glycol or methoxy polyethylene glycol (PEGylation, such as for example to increase the half-life of the protein) or directly or indirectly {e.g., via a linker) fused to another peptide (such as diphtheria toxin, tetanus toxin, KLH, or a purification tag, such as a histidine tag {e.g., 6-His). For example, the disclosure provides isolated Tp0751 protein fragments that include a mutated active site, such as an amino acid sequence comprising the amino acid sequence shown in any of SEQ ID NOS: 4-6 and 8-10, or a sequence comprising at least 90%, at least 95%, or at least 99% sequence identity to any of SEQ ID NOS: 4-6 and 8-10, for example a protein having 0 to 20 conservative amino acid substitutions. In some examples, such variants retain the mutated active site, but have changes at other amino acids {e.g., in the variant protein H174A of SEQ ID NO: 4, E175A of SEQ ID NO: 5, H178A of SEQ ID NO: 6, H175A of SEQ ID NO: 8, E176A of SEQ ID NO: 9, H179A of SEQ ID NO: 10, amino acids 174-178 of SEQ ID NO: 4, amino acids 174-178 of SEQ ID NO: 5, amino acids 174-178 of SEQ ID NO: 6, amino acids 175-179 of SEQ ID NO: 8, amino acids 175-179 of SEQ ID NO: 9, and/or amino acids 175-179 of SEQ ID NO: 10, would not be changed). Pharmaceutical compositions, such as a vaccine, which include one or more isolated Tp0751 protein fragments, isolated nucleic acid molecules that encode Tp0751 protein fragments, or vectors that include the nucleic acid, are provided. The disclosure also provides kits containing such Tp0751 protein fragments. The foregoing and other objects and features of the disclosure will become more apparent from the following detailed description, which proceeds with reference to the accompanying figures.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 provides an overview of the methods used to immunize rabbits and challenge them with T. pallidum.

FIG. 2 is a bar graph showing the titers of rabbits immunized with amino acids (aa) 24-

237 of Tp0751. The bar graph shows the titers for these 6 different rabbits (# 25-30). For each rabbit there are 4 time points. For each time point there are multiple dilutions

FIGS. 3A and 3B are line graphs showing the effect on (A) chancre ulceration and (B) chancre volume in rabbits immunized or not (control) with aa 24-237 of Tp0751 and subsequently challenged with T. pallidum.

SEQUENCE LISTING

The nucleic and amino acid sequences listed in the accompanying sequence listing are shown using standard letter abbreviations for nucleotide bases, and three letter code for amino acids, as defined in 37 C.F.R. 1.822. Only one strand of each nucleic acid sequence is shown, but the complementary strand is understood as included by any reference to the displayed strand. In the accompanying sequence listing:

SEQ ID NO: 1 is an exemplary Tp0751 coding sequence (nt 815821..816534 of GenBank Accession No. AE000520.1)

SEQ ID NO: 2 (GenBank Accession No. NP_219188) is an exemplary full-length Tp0751 amino acid sequence (active site underlined), which includes a signal sequence (amino acids 1-23) which are cleaved off to form the mature protein found in T. pallidum.

1 vnrpllsvag slfvaawaly ifscfqhghv pprripphdt fgalptaalp snardtaahp

61 sdtadntsgs stttdprshg nappapvgga aqthtqppvq tamrialwnr athgeqgalq

121 hllaglwiqt eispnsgdih pllffdreha eitfsrasvq eiflvdsaht hrktvsfltr

181 ntaissirrr levtfeshev ihvravedva rlkigstsmw dgqytryhag pasapsp

SEQ ID NO: 3 is a fragment of Tp0751 (aa 25-237) (native active site underlined). This is encoded by nt 73-711 of SEQ ID NO: 1.

fqhghv pprripphdt fgalptaalp snardtaahp

sdtadntsgs stttdprshg nappapvgga aqthtqppvq tamrialwnr athgeqgalq

hllaglwiqt eispnsgdih pllffdreha eitfsrasvq eiflvdsaht hrktvsfltr

ntaissirrr levtfeshev ihvravedva rlkigstsmw dgqytryhag pasapsp SEQ ID NO: 4 is a fragment of Tp0751 (aa 25-237) with a mutated active site

(underlined) (H174A).

fqhghv pprripphdt fgalptaalp snardtaahp

sdtadntsgs stttdprshg nappapvgga aqthtqppvq tamrialwnr athgeqgalq

hllaglwiqt eispnsgdih pllffdreha eitfsrasvq eiflvdsaht hrktvsfltr

ntaissirrr levtfesaev ihvravedva rlkigstsmw dgqytryhag pasapsp

SEQ ID NO: 5 is a fragment of Tp0751 (aa 25-237) with a mutated active site

(underlined) (E175A).

fqhghv pprripphdt fgalptaalp snardtaahp

sdtadntsgs stttdprshg nappapvgga aqthtqppvq tamrialwnr athgeqgalq

hllaglwiqt eispnsgdih pllffdreha eitfsrasvq eiflvdsaht hrktvsfltr

ntaissirrr levtfeshav ihvravedva rlkigstsmw dgqytryhag pasapsp

SEQ ID NO: 6 is a fragment of Tp0751 (aa 25-237) with a mutated active site

(underlined) (H178A).

fqhghv pprripphdt fgalptaalp snardtaahp

sdtadntsgs stttdprshg nappapvgga aqthtqppvq tamrialwnr athgeqgalq

hllaglwiqt eispnsgdih pllffdreha eitfsrasvq eiflvdsaht hrktvsfltr

ntaissirrr levtfeshev iavravedva rlkigstsmw dgqytryhag pasapsp

SEQ ID NO: 7 is a fragment of Tp0751 (aa 24-237) (native active site underlined). This is encoded by nt 70-711 of SEQ ID NO: 1.

cfqhghv pprripphdt fgalptaalp snardtaahp

sdtadntsgs stttdprshg nappapvgga aqthtqppvq tamrialwnr athgeqgalq

hllaglwiqt eispnsgdih pllffdreha eitfsrasvq eiflvdsaht hrktvsfltr

ntaissirrr levtfeshev ihvravedva rlkigstsmw dgqytryhag pasapsp

SEQ ID NO: 8 is a fragment of Tp0751 (aa 24-237) with a mutated active site

(underlined) (H175A).

cfqhghv pprripphdt fgalptaalp snardtaahp

sdtadntsgs stttdprshg nappapvgga aqthtqppvq tamrialwnr athgeqgalq

hllaglwiqt eispnsgdih pllffdreha eitfsrasvq eiflvdsaht hrktvsfltr

ntaissirrr levtfesaev ihvravedva rlkigstsmw dgqytryhag pasapsp

SEQ ID NO: 9 is a fragment of Tp0751 (aa 24-237) with a mutated active site

(underlined) (E176A).

cfqhghv pprripphdt fgalptaalp snardtaahp

sdtadntsgs stttdprshg nappapvgga aqthtqppvq tamrialwnr athgeqgalq

hllaglwiqt eispnsgdih pllffdreha eitfsrasvq eiflvdsaht hrktvsfltr

ntaissirrr levtfeshav ihvravedva rlkigstsmw dgqytryhag pasapsp

SEQ ID NO: 10 is a fragment of Tp0751 (aa 24-237) with a mutated active site

(underlined) (H179A).

cfqhghv pprripphdt fgalptaalp snardtaahp

sdtadntsgs stttdprshg nappapvgga aqthtqppvq tamrialwnr athgeqgalq hl laglwiqt e i spnsgdih pl l f fdreha e it f srasvq e i f lvdsaht hrktvs f ltr ntai s s irrr levt fe shev iavravedva r lkigst smw dgqytryhag pasapsp

SEQ ID NOS: 11-13 are exemplary purification tags.

DETAILED DESCRIPTION DETAILED DESCRIPTION OF SEVERAL EMBODIMENTS

The following explanations of terms and methods are provided to better describe the present disclosure and to guide those of ordinary skill in the art in the practice of the present disclosure. The singular forms "a," "an," and "the" refer to one or more than one, unless the context clearly dictates otherwise. For example, the term "comprising a Tp0751 fragment" includes single or plural Tp0751 fragments and is considered equivalent to the phrase

"comprising at least one Tp0751 fragment." The term "or" refers to a single element of stated alternative elements or a combination of two or more elements, unless the context clearly indicates otherwise. As used herein, "comprises" means "includes." Thus, "comprising A or B," means "including A, B, or A and B," without excluding additional elements.

Unless explained otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present disclosure, suitable methods and materials are described below. The materials, methods, and examples are illustrative only and not intended to be limiting. All GenBank accession numbers and references provided herein are incorporated by reference.

Adjuvant: A vehicle used to enhance antigenicity. Adjuvants which can be used in combination with the Tp0751 fragments disclosed herein (including the mutated and modified

Tp0751 fragments disclosed herein) include a suspension of minerals (alum, aluminum hydroxide, or phosphate) on which antigen is adsorbed; or water-in-oil emulsion in which antigen solution is emulsified in mineral oil (Freund incomplete adjuvant), sometimes with the inclusion of killed mycobacteria (Freund's complete adjuvant) to further enhance antigenicity

(inhibits degradation of antigen and/or causes influx of macrophages). Immuno stimulatory oligonucleotides (such as those including a CpG motif) can also be used as adjuvants (for example see U.S. Patent No. 6,194,388; U.S. Patent No. 6,207,646; U.S. Patent No. 6,214,806;

U.S. Patent No. 6,218,371; U.S. Patent No. 6,239,116; U.S. Patent No. 6,339,068; U.S. Patent

No. 6,406,705; and U.S. Patent No. 6,429,199). Adjuvants include biological molecules (a "biological adjuvant"), such as costimulatory molecules. Other exemplary adjuvants include IL- 2, RANTES, GM-CSF, TNF-a, IFN-γ, G-CSF, LFA-3, CD72, B7-1, B7-2, OX-40L and 4-1 BBL. Other exemplary adjuvants include chitosan, Bacillus-Calmette-Guerin adjuvant and RIBI adjuvant. Other adjuvants are disclosed herein. Thus, one or more of these adjuvants can be included in the pharmaceutical compositions provided herein that contain one or more Tp0751 fragments.

Antigen: A compound, composition, or substance that can stimulate the production of antibodies or a T cell response in an animal, including compositions that are injected or absorbed into an animal. An exemplary antigen is a native or non-native fragment of Tp0751, such as any of SEQ ID NOS: 3-10 (which can optionally include a tag at the N- or C-terminus, such as a 6xHis tag or other purification tag), as well as a modified Tp0751 protein fragment (such as one modified with polyethylene glycol or methoxy polyethylene glycol (PEGylation) or one directly or indirectly fused to another peptide such as diphtheria toxin, tetanus toxin, KLH, or a purification tag. An antigen reacts with the products of specific humoral or cellular immunity, including those induced by heterologous immunogens. The term "antigen" includes all related antigenic epitopes. "Epitope" or "antigenic determinant" refers to a site on an antigen to which B and/or T cells respond. In one embodiment, T cells respond to the epitope, when the epitope is presented in conjunction with an MHC molecule. Epitopes can be formed both from contiguous amino acids or noncontiguous amino acids juxtaposed by tertiary folding of a protein. Epitopes formed from contiguous amino acids are typically retained on exposure to denaturing solvents whereas epitopes formed by tertiary folding are typically lost on treatment with denaturing solvents. An epitope typically includes at least 3, and more usually, at least 5, about 9, or about 8-10 amino acids in a unique spatial conformation, but is generally not more than 20 amino acids in length. Methods of determining spatial conformation of epitopes include, for example, x-ray crystallography and 2-dimensional nuclear magnetic resonance.

Antibody: Immunoglobulin molecules and immunologically active portions of immunoglobulin molecules, i.e., molecules that contain an antigen binding site that specifically binds (immunoreacts with) an antigen, such as a native or non-native fragment of a Tp0751 protein provided herein.

A naturally occurring antibody (e.g. , IgG, IgM, IgD) includes four polypeptide chains, two heavy (H) chains and two light (L) chains interconnected by disulfide bonds. However, it has been shown that the antigen-binding function of an antibody can be performed by fragments of a naturally occurring antibody. Thus, these antigen-binding fragments are also intended to be designated by the term "antibody." Specific, non-limiting examples of binding fragments encompassed within the term antibody include (i) a Fab fragment consisting of the V _L , V _R , C _L and C _H I domains; (ii) an Fd fragment consisting of the V _H and C _H I domains; (iii) an Fv fragment consisting of the VL and VH domains of a single arm of an antibody, (iv) a dAb fragment (Ward et al., Nature 341:544-546, 1989) which consists of a V _H domain; (v) an isolated

complementarity determining region (CDR); and (vi) a F(ab') ₂ fragment, a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region.

Immunoglobulins and certain variants thereof are known and many have been prepared in recombinant cell culture (e.g., see U.S. Patent No. 4,745,055; U.S. Patent No. 4,444,487; WO 88/03565; EP 256,654; EP 120,694; EP 125,023; Faoulkner et al., Nature 298:286, 1982;

Morrison, J. Immunol. 123:793, 1979; Morrison et &l., Ann Rev. Immunol 2:239, 1984).

Humanized antibodies and fully human antibodies are also known in the art.

Consists Essentially Of/Consists Of: With regard to a protein (such as a native or non- native fragment of Tp0751), a protein that consists essentially of a specified amino acid sequence if it does not include any additional amino acid residues. However, the protein can include additional non-peptide components, such as labels (for example, fluorescent, radioactive, or solid particle labels), PEG, sugars or lipids. With regard to a protein that consists of a specified amino acid sequence does not include any additional amino acid residues, nor does it include additional non-peptide components, such as lipids, PEG, sugars or labels.

Degenerate variant: A polynucleotide encoding fragment of Tp0751 that includes a sequence that is degenerate as a result of the genetic code. There are 20 natural amino acids, most of which are specified by more than one codon. Therefore, all degenerate nucleotide sequences are included in this disclosure as long as the amino acid sequence of the fragment of Tp0751 protein encoded by the nucleotide sequence is unchanged.

Immune response: A response of a cell of the immune system, such as a B cell, T cell, or monocyte, to a stimulus. In one embodiment, the response is specific for a particular antigen (an "antigen- specific response"). In one embodiment, an immune response is a T cell response, such as a CD4+ response or a CD8+ response. In another embodiment, the response is a B cell response, and results in the production of specific antibodies (such as T. pallidum- or Tp0751- specific antibodies).

Immunogenic composition: A composition, such as a composition comprising a native or non-native fragment of Tp0751 protein or a nucleic acid encoding the native or non-native fragment of Tp0751, that induces a measurable T cell response against cells expressing Tp0751 protein, or induces a measurable B cell response (such as production of antibodies that specifically bind Tp0751) against a Tp0751 protein. For in vitro use, the immunogenic composition can consist of the isolated nucleic acid, vector including the nucleic acid/or immunogenic protein. For in vivo use, the immunogenic composition will typically include the nucleic acid, vector including the nucleic acid, and or immunogenic protein, in pharmaceutically acceptable carriers, and/or other agents. An immunogenic composition can optionally include an adjuvant, a costimulatory molecule, a nucleic acid encoding a costimulatory molecule, or combinations thereof.

Immunogenic Protein: A protein which includes an allele- specific motif or other sequence such that the peptide will bind an MHC molecule and induce a T cell response, or a B cell response (e.g., antibody production) against the antigen. An exemplary immunogenic protein is a native or non-native fragment of Tp0751 (such as any of SEQ ID NOS: 3-10, or shorter immunogenic fragments thereof).

Immunogenic fragments of native or non-native Tp0751 cause induction of an immune response, as measured by clinical response (for example an increase in a population of immune cells, increased cytolytic activity against cells that express Tp0751, increased production of Tp0751 antibodies, or measurable reduction of chancre volume or ulcerations, or combinations thereof). Immunogenic proteins can also be made from nucleic acids. Examples of a nucleic acid based therapeutically active molecule is a nucleic acid sequence that encodes a native or non-native Tp0751 protein fragment, wherein the nucleic acid sequence is operably linked to a control element such as a promoter.

Isolated: A biological component (such as a nucleic acid molecule or protein) that has been substantially separated, produced apart from, or purified away from other biological components in the cell of the organism in which the component naturally occurs, such as other chromosomal and extrachromosomal DNA and RNA, and proteins. Nucleic acid molecules and proteins which have been "isolated" include nucleic acid molecules and proteins purified by standard purification methods. The term also includes nucleic acid molecules and proteins prepared by recombinant expression in a host cell as well as chemically synthesized nucleic acid molecules and peptides. For example, an isolated or "purified" Tp0751 protein or fragment thereof can be substantially free of other proteins, lipids, carbohydrates or other materials with which it was associated. In one embodiment, a Tp0751 protein fragment is at least 50%, for example at least 80%, at least 90%, or at least 95% free of other proteins, lipids, carbohydrates or other materials with which it was associated (such as a cell lysate).

pDEST17: A gateway expression vector that is used in universal cloning techniques allowing the transfer of DNA between different cloning vectors while maintaining the reading frame, designed by Invitrogen Life Technologies, Cat. No. 11803012. Pharmaceutically acceptable carriers: The pharmaceutically acceptable carriers of use are conventional. Remington 's Pharmaceutical Sciences, by E. W. Martin, Mack Publishing Co., Easton, PA, 15th Edition (1975), describes compositions and formulations suitable for pharmaceutical delivery of the native or non-native Tp0751 protein fragments herein disclosed.

In general, the nature of the carrier will depend on the particular mode of administration being employed. For instance, parenteral formulations usually comprise injectable fluids that include pharmaceutically and physiologically acceptable fluids such as water, physiological saline, balanced salt solutions, aqueous dextrose, glycerol or the like as a vehicle. For solid compositions (such as powder, pill, tablet, or capsule forms), conventional non-toxic solid carriers can include, for example, pharmaceutical grades of mannitol, lactose, starch, or magnesium stearate. In addition to biologically neutral carriers, pharmaceutical compositions to be administered can contain minor amounts of non-toxic auxiliary substances, such as wetting or emulsifying agents, preservatives, and pH buffering agents and the like, for example sodium acetate or sorbitan monolaurate.

A "therapeutically effective amount" is a quantity of a composition or a cell to achieve a desired effect in a subject being treated. For instance, this can be the amount of a native or non-native Tp0751 protein fragment or a vector encoding such a protein, necessary to induce an immune response, reduce chancre volume (e.g., relative to no administration of the native or non-native Tp0751 protein fragment), reduce the % of chancres that ulcerate(e.g., relative to no administration of the native or non-native Tp0751 protein fragment), reduce orchitis (e.g., relative to no administration of the native or non-native Tp0751 protein fragment), reduce dissemination of T. pallidum to lymph nodes and other tissues and organs (e.g., relative to no administration of the native or non-native Tp0751 protein fragment), measurably improve outward symptoms of a T. pallidum infection (e.g., relative to no administration of the native or non-native Tp0751 protein fragment),or combinations thereof. When administered to a subject, a dosage will generally be used that will achieve target tissue concentrations (for example, in lymphocytes) that has been shown to achieve an in vitro effect.

Recombinant: A recombinant protein or nucleic acid is one that has a sequence that is not naturally occurring or has a sequence that is made by an artificial combination of two otherwise separated segments of sequence. This artificial combination can accomplished by chemical synthesis or other methods known in the art, such as by the artificial manipulation of isolated segments of nucleic acids, e.g., by genetic engineering techniques.

Sequence identity/similarity: The identity/similarity between two or more nucleic acid sequences, or two or more amino acid sequences, is expressed in terms of the identity or similarity between the sequences. Sequence identity can be measured in terms of percentage identity; the higher the percentage, the more identical the sequences are. Sequence similarity can be measured in terms of percentage similarity (which takes into account conservative amino acid substitutions); the higher the percentage, the more similar the sequences are.

Methods of alignment of sequences for comparison are well known in the art. Various programs and alignment algorithms are described in: Smith & Waterman, Adv. Appl. Math. 2:482, 1981; Needleman & Wunsch, J. Mol. Biol. 48:443, 1970; Pearson & Lipman, Proc. Natl. Acad. Sci. USA 85:2444, 1988; Higgins & Sharp, Gene, 73:237-44, 1988; Higgins & Sharp, CABIOS 5:151-3, 1989; Corpet et al., Nuc. Acids Res. 16: 10881-90, 1988; Huang et al.

Computer Appls. in the Biosciences 8, 155-65, 1992; and Pearson et al., Meth. Mol. Bio. 24:307- 31, 1994. Altschul et al, J. Mol. Biol. 215:403-10, 1990, presents a detailed consideration of sequence alignment methods and homology calculations.

The NCBI Basic Local Alignment Search Tool (BLAST) (Altschul et al, J. Mol. Biol. 215:403-10, 1990) is available from several sources, including the National Center for

Biological Information (NCBI, National Library of Medicine, Building 38A, Room 8N805, Bethesda, MD 20894) and on the Internet, for use in connection with the sequence analysis programs blastp, blastn, blastx, tblastn and tblastx. Additional information can be found at the NCBI web site.

BLASTN is used to compare nucleic acid sequences, while BLASTP is used to compare amino acid sequences. If the two compared sequences share homology, then the designated output file will present those regions of homology as aligned sequences. If the two compared sequences do not share homology, then the designated output file will not present aligned sequences.

Once aligned, the number of matches is determined by counting the number of positions where an identical nucleotide or amino acid residue is presented in both sequences. The percent sequence identity is determined by dividing the number of matches either by the length of the sequence set forth in the identified sequence, or by an articulated length (such as 100

consecutive nucleotides or amino acid residues from a sequence set forth in an identified sequence), followed by multiplying the resulting value by 100. For example, a nucleic acid sequence that has 1166 matches when aligned with a test sequence having 1554 nucleotides is 75.0 percent identical to the test sequence (1166÷1554* 100=75.0). The percent sequence identity value is rounded to the nearest tenth. For example, 75.11, 75.12, 75.13, and 75.14 are rounded down to 75.1, while 75.15, 75.16, 75.17, 75.18, and 75.19 are rounded up to 75.2. The length value will always be an integer. In another example, a target sequence containing a 15- nucleotide region that aligns with 20 consecutive nucleotides from an identified sequence as follows contains a region that shares 75 percent sequence identity to that identified sequence (that is, 15÷20* 100=75).

For comparisons of amino acid sequences of greater than about 30 amino acids, the Blast 2 sequences function is employed using the default BLOSUM62 matrix set to default parameters, (gap existence cost of 11, and a per residue gap cost of 1). Homologs are typically characterized by possession of at least 30% sequence identity or more counted over the full- length alignment with an amino acid sequence using the NCBI Basic Blast 2.0, gapped blastp with databases such as the nr or swissprot database. Queries searched with the blastn program are filtered with DUST (Hancock and Armstrong, 1994, Comput. Appl. Biosci. 10:67-70). Other programs use SEG. In addition, a manual alignment can be performed. Proteins with even greater similarity will show increasing percentage identities when assessed by this method, such as at least about 75%, 80%, 85%, 90%, 95%, 98%, or 99% sequence identity with a Tp0751 protein, or fragments thereof. Thus in one example, a Tp0751 protein fragment has at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity to any of SEQ ID NOs: 3-10 and retains the ability to stimulate an immune response against Tp0751 in vivo.

One indication that two nucleic acid molecules are closely related is that the two molecules hybridize to each other under stringent conditions, as described above. Nucleic acid sequences that do not show a high degree of identity may nevertheless encode identical or similar (conserved) amino acid sequences, due to the degeneracy of the genetic code. Changes in a nucleic acid sequence can be made using this degeneracy to produce multiple nucleic acid molecules that all encode substantially the same protein. Such homologous nucleic acid sequences can, for example, possess at least about 60%, 70%, 80%, 90%, 95%, 98%, or 99% sequence identity with a Tp0751 sequence as determined by this method. Thus in one example, a Tp0751 nucleic acid encoding sequence has at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity to SEQ ID NO: 1 or nucleotides 70-711 or 73-711 of SEQ ID NO: 1, and encodes a native or non-native Tp0751 protein or fragment thereof (such as any of SEQ ID NOS: 3-10). An alternative (and not necessarily cumulative) indication that two nucleic acid sequences are substantially identical is that the polypeptide which the first nucleic acid encodes is immunologically cross reactive with the polypeptide encoded by the second nucleic acid;

Subject: Living multi-cellular vertebrate organisms, a category that includes human and non-human mammals, for example mammals that get syphilis (such as humans and rabbits). Tp0751 (also known as pallilysin): A surface antigen of T. pallidum, which has been reported previously to bind to laminin. Antibodies against this adhesin are present in serum from both natural and experimental T. pallidum infections. The term Tp0751 includes a Tp0751 gene, cDNA, mRNA, or protein. Nucleic acid and protein sequences for Tp0751 are publicly available. For example, nt 815821..816534 of GenBank Accession No. AE000520.1 discloses a Tp0751 nucleic acid coding sequence, and GenBank Accession Nos.: NP_219188.1 and

AAC65720.1 disclose Tp0751 protein sequences, all of which are incorporated by reference as provided by GenBank on July 12, 2013.

Tp0751 begins as pre-protein (e.g., SEQ ID NO: 2), from which a mature protein found in T. pallidum is generated by cleaving off the signal sequence (amino acids 1-23). Fragments of Tp0751 refer to native fragments of the pre-protein or the mature protein (e.g., SEQ ID NO: 3 or 7) as well as non-native fragments containing a mutated active site (e.g., SEQ ID NO: 4, 5, 6, 8, 9, or 10). One skilled in the art will appreciate that variations can be made to these sequences, as long as the Tp0751 protein fragment can stimulate an immune response in vivo. In certain examples, a variant Tp0751 protein fragment has at least 80% sequence identity, for example at least 85%, at least 90%, at least 95%, at least 97%, at least 98%, or at least 99% sequence identity to any of SEQ ID NOS: 3-10 and can stimulate an immune response in vivo, for example a protective immune response against T. pallium infection. In one example, a Tp0751 protein fragment (such as any of SEQ ID NOS: 3-10) can contain 1 to 20 conservative amino acid substitutions, and can further include a tag, such as purification tag, such as an N-terminal or C-terminal histidine tag (such as a histidine tag having 3 to 6 histidine residues, such as 3-, 4-, 5- or 6-His).

In some examples, a variant non-native Tp0751 protein fragment retains the mutated active site, but has changes at one or more other amino acids, such as conservative or non- conservative changes to 1 to 20, 1 to 10, or 1 to 5 amino acids. For example, a variant non- native Tp0751 protein fragment that retains the mutated active site can retain H174A of SEQ ID NO: 4, E175A of SEQ ID NO: 5, H178A of SEQ ID NO: 6, H175A of SEQ ID NO: 8, E176A of SEQ ID NO: 9, H179A of SEQ ID NO: 10, amino acids 174-178 of SEQ ID NO: 4, amino acids 174-178 of SEQ ID NO: 5, amino acids 174-178 of SEQ ID NO: 6, amino acids 175-179 of SEQ ID NO: 8, amino acids 175-179 of SEQ ID NO: 9, and/or amino acids 175-179 of SEQ ID NO: 10 (that is, these sequences would not be changed in the variant peptide, e.g., one having at least 80%, at least 90%, at least 95% etc. sequence identity to SEQ ID NO: 4, 5, 6, 8, 9, or 10). Immunogenic Tp0751 Protein Fragments and Coding Sequences

Proteins

The present disclosure provides isolated or purified Tp0751 fragments, such as native sequences shown in SEQ ID NO: 3 and 7, amino acids 78-237 of SEQ ID NO: 2, as well as non- native fragments that include one or more mutations in the active site (native site is HEXXH, so 1, 2, or all 3 of HI, E2 or H5 can be mutated, for example mutated to an alanine), such as those shown in SEQ ID NOs: 4-6 and 8- 10. In some examples, these proteins include other mutations, such as an alanine at position 46, 78, 92 or combinations thereof (wherein the numbering is relative to SEQ ID NO: 2). For example, a variant non-native Tp0751 protein fragment can retain the mutated active site, but has changes at one or more other amino acids, such as conservative or non-conservative changes to 1 to 20, 1 to 10, or 1 to 5 amino acids. For example, a variant non-native Tp0751 protein fragment that retains the mutated active site can retain H174A of SEQ ID NO: 4, E175A of SEQ ID NO: 5, H178A of SEQ ID NO: 6, H175A of SEQ ID NO: 8, E176A of SEQ ID NO: 9, H179A of SEQ ID NO: 10, amino acids 174-178 of SEQ ID NO: 4, amino acids 174- 178 of SEQ ID NO: 5, amino acids 174- 178 of SEQ ID NO: 6, amino acids 175- 179 of SEQ ID NO: 8, amino acids 175-179 of SEQ ID NO: 9, and/or amino acids 175-179 of SEQ ID NO: 10 (that is, these sequences would not be changed in the variant peptide, e.g., one having at least 80%, at least 90%, at least 95% etc. sequence identity to SEQ ID NO: 4, 5, 6, 8, 9, or 10).

In some examples, a Tp0751 fragment includes the C-terminal end of the mature Tp0751 protein, such as one that includes at least 30, at least 35, at least 40, at least 45, at least 50 or at least 55 consecutive C-terminal amino acids (such as the region of amino acids 150-237, 180- 237, or 190-237 of SEQ ID NO: 2) (as removing the C-terminal end can result in insoluble expression). In one example, the Tp0751 fragment includes the HEXXH metalloprotease active site within the C terminus of the protein (though this site may be mutated as discussed herein).

These Tp0751 fragments can be used as an immunogen, such as a vaccine, in the methods provided herein. Thus, the Tp0751 fragments disclosed herein can be used to induce an immune response (are immunogenic), wherein the Tp0751 fragment can produce a Tp0751- specific CD4+ T cell response. In some embodiments, the Tp0751 fragment produces a Tp0751- specific B cell response, a Tp0751- specific CD4+ T cell response, a Tp0751- specific CD8+ T cell response, or combinations thereof. For example, such Tp0751 fragments can be used to illicit a B cell response (e.g., production of serum antibodies which have neutralizing activity for T. pallidum), and thus can be used as a vaccine against T. pallidum infection. One skilled in the art will recognize that such sequences can be altered using routine methods in the art (such as recombinant molecular biology methods), while not significantly decreasing the desired activity of the Tp0751 protein fragment, such as the ability to produce an immune response (e.g., generate antibodies specific for Tp0751, such as those having neutralizing activity for T. pallidum) when administered to a mammal. The immunogenicity of a Tp0751 protein fragment variant can be assessed by determining whether it is recognized by Tp0751 antibodies (such as Tp0751 antibodies present in a subject having syphilis). In some examples, a Tp0751 protein fragment binds MHC Class II, such as one encoded by a HLA-DP, HLA-DR, HLA-B, HLA-DQA1 or HLA-DQB 1 allele.

Thus, the present disclosure provides variants of the disclosed Tp0751 fragment sequences, such as a Tp0751 protein sequence having at least 60%, at least 70%, at least 80%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to any of SEQ ID NOs: 3- 10 or such identity to a protein comprising or consisting of amino acids 78-237 of SEQ ID NO: 2. In one example, a Tp0751 fragment includes, consists of, or consists essentially of, an amino acid sequence at least at least 90% identical to SEQ ID NO: 1, for example a polypeptide that is about 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identical to any of SEQ ID NOs: 3- 10. For example, the peptide sequence shown in any of SEQ ID NOs: 3-10 (or amino acids 78- 237 of SEQ ID NO: 2) can be modified by making one or more conservative amino acid substitutions, such as 1 to 20, 1 to 15, 1 to 12, 1 to 10, or 1 to 5 conservative amino acid substitutions, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 conservative amino acid substitutions, while retaining the ability to produce an immune response when administered to a mammal. In some examples, amino acids 174, 175 and 178 of SEQ ID NOS: 4-6, respectively (such as any or all of amino acids 174- 178) are not changed, and in some examples amino acids 175, 176 and 177 of SEQ ID NOS: 8- 10, respectively (such as any or all of amino acids 175- 176) are not changed. For example, a variant non-native Tp0751 protein fragment that retains the mutated active site can retain H174A of SEQ ID NO: 4, E175A of SEQ ID NO: 5, H178A of SEQ ID NO: 6, H175A of SEQ ID NO: 8, E176A of SEQ ID NO: 9, H179A of SEQ ID NO: 10, amino acids 174-178 of SEQ ID NO: 4, amino acids 174-178 of SEQ ID NO: 5, amino acids 174- 178 of SEQ ID NO: 6, amino acids 175- 179 of SEQ ID NO: 8, amino acids 175- 179 of SEQ ID NO: 9, and/or amino acids 175-179 of SEQ ID NO: 10 (that is, these sequences would not be changed in the variant peptide, e.g. , one having at least 80%, at least 90%, at least 95% etc. sequence identity to SEQ ID NO: 4, 5, 6, 8, 9, or 10).

Examples of conservative substitutions are shown in Table 1 : Table 1 : Exemplary conservative amino acid substitutions

Original Residue Conservative Substituti

Ala Ser

Arg Lys

Asn Gin, His

Asp Glu

Cys Ser

Gin Asn

Glu Asp

His Asn; Gin

He Leu, Val

Leu He; Val

Lys Arg; Gin; Glu

Met Leu; He

Phe Met; Leu; Tyr

Ser Thr

Thr Ser

Trp Tyr

Tyr Trp; Phe

Val He; Leu

In some examples, a Tp0751 fragment includes at least at least 15, at least 20, at least 30, at least 40, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 210, or at least 220 amino acids of a full-length Tp0751 protein (e.g., SEQ ID NO: 2). The Tp0751 fragment can include 15, 20, 30, 40, 50, 60, 70, 80, 90, 100, 150, 200, 210, 212, 213, or 220 contiguous amino acids of a full-length Tp0751 protein (e.g., SEQ ID NO: 2). In some embodiments, a Tp0751 fragment is 15-212, 20-212, 30-212, 40-212, 50-212, 60-212, 70-212, 80-212, 90-212, 100-212, or 200-212 amino acids of a full-length Tp0751 protein (e.g., SEQ ID NO: 2). In other embodiments, a Tp0751 fragment is 15-213, 20-213, 30-213, 40-213, 50-213, 60-213, 70-213, 80-213, 90-213, 100-213, or 200-213 amino acids of a full-length Tp0751 protein (e.g., SEQ ID NO: 2).

In one example, a Tp0751 fragment is part of a fusion protein. Such a fusion protein can include the Tp0751 fragment provided herein and a second heterologous moiety, such as a myc protein, KLH, a toxin (e.g., DT, tetanus), an enzyme or a carrier (such as a hepatitis carrier protein or bovine serum albumin) covalently linked to the Tp0751 fragment. When bound to a carrier, the Tp0751 fragment becomes more immunogenic. Carriers can increase the

immunogenicity of the bound molecule and/or to elicit higher titers of antibodies against the carrier which are therapeutically beneficial. Covalent linking of a molecule to a carrier can confer enhanced immunogenicity and T cell dependence (see Pozsgay et al., PNAS 96:5194-97, 1999; Lee et al., J. Immunol. 116: 1711-18, 1976; Dintzis et al., PNAS 73:3671-75, 1976).

Useful carriers include polymeric carriers, which can be natural (for example, polysaccharides, polypeptides or proteins from bacteria or viruses), semi- synthetic or synthetic materials containing one or more functional groups to which a reactant moiety can be attached. Bacterial products and viral proteins (such as hepatitis B surface antigen and core antigen) can also be used as carriers, as well as proteins from higher organisms such as keyhole limpet hemocyanin (KLH), horseshoe crab hemocyanin, edestin, mammalian serum albumins, and mammalian immunoglobulins. Suitable carriers include, but are not limited to, a hepatitis B small envelope protein HBsAg. This protein has the capacity to self-assemble into aggregates and can form viral-like particles. The preparation of HBsAg is well documented, see for example European Patent Application Publication No. EP-A-0 226 846, European Patent Application Publication No. EP-A-0 299 108 and PCT Publication No. WO 01/117554, and the amino acid sequence disclosed, for example, in Tiollais et al., Nature, 317:489, 1985, and European Patent

Publication No. EP-A-0 278 940, and PCT Publication No. WO 91/14703.

In some examples, a Tp0751 fragment (such as any of SEQ ID NOs: 3-10) includes a tag (for example to assist in purification of the protein), such as a C-terminal or N-terminal tag, such as an N-terminal histidine tag. In some examples, the tag is included to aid or permit isolation of the protein, and may or may not be removed before the use of the Tp0751 protein fragment as an immunogen. In some examples, the purification tag is about 3-20 amino acids in length, such as 3 to 10 or 4-6 amino acids. In one example the purification tag is a polyhistidine tag, such as 6-His, which permits nickel purification. Other exemplary purification tags that can be used include but are not limited to: glutathione-S-transferase (GST), a myc-Tag (EQKLISEED; SEQ ID NO: 11), FLAG-Tag (DYKDDDDK; SEQ ID NO: 12), HA-tag, S-tag (Lys-Glu-Thr-Ala- Ala-Ala-Lys-Phe-Glu-Arg-Gln-His-Met-Asp-Ser; SEQ ID NO: 13), and streptavidin. Thus, in some examples, the fusion protein includes a Tp0751 fragment and six sequential histidine residues, a β-galactosidase amino acid sequence, and/or an immunoglobulin amino acid sequence.

Nucleic acid molecules

Also provided are isolated nucleic acid molecules that encode Tp0751 protein fragments, such as nucleotides 70 -711 or 73-711 of SEQ ID NO: 1, which encode SEQ ID NOS: 7 and 3, respectively. In some example, such sequences further include a tag coding sequence at the 3'- or 5'-end. One skilled in the art will recognize that coding sequences for SEQ ID NOS: 4-6 and 8-10 can be readily determined using the genetic code and routine methods. Examples of isolated nucleic acid molecules include cDNA and mRNA.

One skilled in the art will recognize that such sequences can be altered using routine methods in the art (such as recombinant molecular biology methods), while still encoding a Tp0751 protein fragment that retains the ability to function as an immunogen using the disclosed methods. Thus, the present disclosure provides variants of the disclosed Tp0751 nucleic acid sequences, such as a Tp0751 nucleic acid sequence having at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity to nucleotides 70 -711 or 73-711 SEQ ID NO: 1.

For example, the sequence shown in nucleotides 70 -711 or 73-711 of SEQ ID NO: 1 can be modified by making one or more nucleotide substitutions, which in some examples will not change the encoded peptide sequence due to the degeneracy of the code (e.g., silent mutation), and in other examples will change the sequence of the encoded peptide. Therefore, nucleotides 70 -711 or 73-711 of SEQ ID NO: 1 can be modified to result in 1 to 20, 1 to 15, 1 to 12, 1 to 10, or 1 to 5 conservative amino acid substitutions, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 conservative amino acid substitutions, while retaining the ability to encode a Tp0751 peptide fragment that retains the ability to function as an immunogen using the disclosed methods.

In one example, a Tp0453 nucleic acid sequence having at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity to nucleotides 70 -711 or 73-711 of SEQ ID NO: 1, can encode a Tp0751 peptide fragment having at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity to any of SEQ ID NOs: 3-10, while retaining the ability to encode a Tp0751 peptide fragment that retains the ability to function as an immunogen using the disclosed methods. In some examples, a nucleic acid encoding a variant non-native Tp0751 protein fragment has a coding sequence that retains the mutated active site, such as H174A of SEQ ID NO: 4, E175A of SEQ ID NO: 5, H178A of SEQ ID NO: 6, H175A of SEQ ID NO: 8, E176A of SEQ ID NO: 9, H179A of SEQ ID NO: 10, amino acids 174-178 of SEQ ID NO: 4, amino acids 174-178 of SEQ ID NO: 5, amino acids 174- 178 of SEQ ID NO: 6, amino acids 175-179 of SEQ ID NO: 8, amino acids 175- 179 of SEQ ID NO: 9, and/or amino acids 175- 179 of SEQ ID NO: 10 (that is, these coding sequences would not be changed in the variant peptide, unless the change was a silent mutation).

In addition, nucleotides 70-711 or 73-711 of SEQ ID NO: 1 can be modified by making one or more nucleotide (nt) changes without changing the length of the nucleic acid. Therefore, nucleotides 70 -711 or 73-711 of SEQ ID NO: 1 can be modified by making 1 to 100, 1 to 50, 1 to 20, 1 to 15, 1 to 12, 1 to 10, or 1 to 5 nt substitutions, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 30, 40, 50, 60 70, 80, 90, or 100 nt substitutions, while retaining the ability to encode a Tp0751 peptide fragment that retains the ability to function as an immunogen using the disclosed methods.

Vectors that include a nucleic acid that encodes a Tp0751 protein fragment (such as any of SEQ ID NOs: 3-10) are also provided. In one example, the vector is pDEST17. Thus, provided is a pDEST17 vector and nucleotides 70-711 or 73-711 of SEQ ID NO: 1.

Also provided are cells that include the vectors and a Tp0751 protein fragment coding sequences (such as nucleotides 70-711 or 73-711 of SEQ ID NO: 1). In one example the cell is a prokaryotic cell, such as E. coli. Other exemplary cells include insect and yeast cells.

Compositions

Pharmaceutical compositions, which include one or more isolated Tp0751 protein fragments, isolated nucleic acid molecules that encode Tp0751 protein fragments, such as a Tp0751 peptide fragment having at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity to any of SEQ ID NOs: 3-10 or a coding sequence thereof, or vectors that include the nucleic acid, are provided. For example, such a composition can include a pharmaceutically acceptable carrier, such as water, oil, a water and oil emulsion, or saline.

Vaccines, which include one or more isolated Tp0751 protein fragments, isolated nucleic acid molecules that encode Tp0751 protein fragments, or vectors that include the nucleic acid, are provided. For example, such a vaccine composition can include an adjuvant, such as TiterMax Gold (Sigma). In some examples, vaccines include a pharmaceutically acceptable carrier, such as water, oil, a water and oil emulsion, or saline.

Generation of Immune Response using Tp0751 Fragments

The native and non-native Tp0751 protein fragments disclosed herein (such as those having at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity to any of SEQ ID NOs: 3-10, which in some examples retain a mutated binding site), nucleic acids encoding the proteins, or host cells including these nucleic acids can be used to generate an immune response in a subject, such as, but not limited to, a Tp0751- specific CD4+ T cell response, a Tp0751-CD8+ T cell response, a humoral (antibody) response, or combinations thereof. The subject can be a mammal, such as a human, that is at risk for T. pallidum infection. The methods include administering to a subject a therapeutically effective amount of one or more of the native or non-native Tp0751 fragments disclosed herein, nucleic acids encoding these proteins, host cells, such as Listeria or Salmonella host cells, dendritic cells presenting epitopes of the Tp0751 protein fragment, and/or vectors including these nucleic acids, in order to generate an immune response. Thus, a therapeutic composition (such as a vaccine) can include a native or non-native Tp0751 protein fragment, a nucleic acid encoding a such a protein, a vector including the nucleic acid, or a host cell expressing the Tp0751 protein fragment. These compositions can be used in combination. The methods can include selecting a subject in need of treatment, such as a subject at risk for T. pallidum infection, such as a subject who is or may become sexually active.

In exemplary applications, compositions are administered to a subject in an amount sufficient to raise an immune response to Tp0751 -expressing cells, such as a B cell response. Administration induces a sufficient immune response to protect the subject from subsequent T. pallidum infection. Such protection need not be 100%.

For example, such a sufficient immune response can include one that (1) reduces or eliminates chancre formation, such as reduces the volume of a chancre by at least 20%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, or at least 90%, at least 100%, such as at least 1.5 fold or at least 2-fold, such as at least 10 days or at least 14 days following subsequent T. pallidum infection (for example relative to no Tp0751 fragment administration); (2) reduces the % of chancres that are ulcerated, such as reduces the % ulceration of a chancres by at least 20%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, or at least 90%, at least 100%, such as at least 1.5 fold, at least 2-fold, at least 3 -fold, at least 4-fold, or at least 5-fold, such as at least 10 days or at least 14 days following subsequent T. pallidum infection (for example relative to no Tp0751 fragment administration); (3) reduces the % of darkfield positive lesions {i.e., treponemes present), such as reduces the % of treponemes or darkfield positive lesions by at least 20%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, or at least 90%, at least 100%, such as at least 1.5 fold, at least 2-fold, at least 3- fold, at least 4-fold, or at least 5-fold, such as at least 10 days or at least 14 days following subsequent T. pallidum infection (for example relative to no Tp0751 fragment administration); (4) reduces T. pallidum infection disseminated to lymph nodes (such as popliteal lymph nodes) following subsequent T. pallidum infection, such as reduces the T. pallidum infection in lymph nodes and other organs and tissues (such as popliteal lymph nodes) by at least 20%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, or at least 90%, at least 100%, such as at least 1.5 fold, at least 2-fold, at least 3-fold, at least 4-fold, or at least 5-fold, such as at least 100 days or at least 180 days following subsequent T. pallidum infection (for example relative to no Tp0751 fragment administration); (5) reduces orchitis following subsequent T. pallidum infection, such as reduces orchitis by at least 20%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, or at least 90%, at least 100%, , such as at least 10 days or at least 30 days following subsequent T. pallidum infection (for example relative to no Tp0751 fragment administration); (6) reduces seroconversion following subsequent T. pallidum infection, such as reduces seroconversion by at least 20%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, or at least 90%, at least 100%, , such as at least 60 days or at least 180 days following subsequent T. pallidum infection (for example relative to no Tp0751 fragment administration), (7) reduces the number of necrotic lesions following subsequent T. pallidum infection, such as reduces necrotic lesions by at least 20%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, or at least 90%, at least 100%, , such as at least 10 days or at least 30 days following subsequent T. pallidum infection (for example relative to no Tp0751 fragment administration)or combinations thereof.

Amounts effective for this use will depend upon the general state of the patient's health, and the robustness of the patient's immune system. In one example, a therapeutically effective amount of the composition is that which provides either subjective relief of a symptom(s) or an objectively identifiable improvement as noted by the clinician or other qualified observer. In one specific, non-limiting example, a pharmaceutical composition for injection (such as sc, iv or im) administration includes about 0.1 μg to 10 mg (such as 0.1 μg to 5 mg, 0.5 μg to 1 mg or 0.5 μg to 50 μg) of immunogenic Tp0751 protein fragment per subject. Dosages from 0.1 up to about 100 mg per subject can be used, particularly if the agent is administered to a secluded site and not into the circulatory or lymph system, such as into a body cavity or into a lumen of an organ. Actual methods for preparing administrable compositions will be known or apparent to those skilled in the art and are described in more detail in such publications as Remingtons Pharmaceutical Sciences, 19 ^th Ed., Mack Publishing Company, Easton, Pennsylvania, 1995. In some examples the patient receives two or more doses of the Tp0751 fragment, such as an initial dose, followed by 1, 2, or 3 booster doses. In some examples, the patient receives no more than 3 doses of the Tp0751 fragment over no longer than a 1 year timeframe.

In some examples, to monitor the antibody response of subjects administered the Tp0751 fragments of the disclosure, antibody levels may be determined. In some instances it will be sufficient to assess the antibody titer in serum or plasma obtained from such a subject. Decisions as to whether to administer booster inoculations or to change the amount of the composition administered to the individual may be at least partially based on the level. The level can be based on routine assays, such as an immunobinding (e.g., ELISA) assay which measures the concentration of antibodies in the serum which bind to a specific antigen, Tp0751. The ability to neutralize in vitro and in vivo biological effects of the T. pallidum can also be assessed to determine the effectiveness of the treatment.

The composition can be administered by any means known to one of skill in the art (see Banga, A., "Parenteral Controlled Delivery of Therapeutic Peptides and Proteins," in

Therapeutic Peptides and Proteins, Technomic Publishing Co., Inc., Lancaster, PA, 1995). Thus, the composition can be administered either locally or systemically, such as by

intramuscular, subcutaneous, intraperitoneal, intradermal, or intravenous injection, but even oral, nasal, transdermal, vaginal, or anal administration is contemplated. In one embodiment, administration is by subcutaneous or intramuscular injection.

When the Tp0751 protein fragment is administered, to extend the time during which protein is available to stimulate a response, the protein can be provided as an implant, an oily injection, in a liposome, or as a particulate system. The particulate system can be a

microparticle, a microcapsule, a microsphere, a nanocapsule, or similar particle, (see, e.g., Banga, supra). A particulate carrier based on a synthetic polymer has been shown to act as an adjuvant to enhance the immune response, in addition to providing a controlled release.

Adjuvants can also be used in combination with the protein, including, for example, chitosan, aluminum salts, an immunostimulatory oligodeoxynucletoide, liposomes and/or one or more cytokines. The Tp0751 protein fragment can be administered in a liposome.

Optionally, one or more immunostimulatory molecules, such as IL-2, IL-6, IL-12, LFA (for example, LFA-1, LFA-2 and/or LFA-3), CD72, RANTES, G-CSF, GM-CSF, TNF-a, IFN- γ, ICAM-1, B7-1, B7-2, other B7 related molecules, OX-40L and/or or 41 BBL, or

combinations of these molecules, can be used as biological adjuvants (see, for example,

Salgaller et al, 1998, J. Surg. Oncol. 68(2): 122-38; Lotze et al., 2000, Cancer J Sci. Am.

6(Suppl l):S61-6; Cao et al., 1998, Stem Cells 16(Suppl l):251-60; Kuiper et al., 2000, Adv. Exp. Med. Biol. 465:381-90). These molecules can be administered systemically (or locally) to the host. In some examples, IL-2, RANTES, GM-CSF, TNF-a, IFN-γ, G-CSF, LFA-3, CD72, B7-1, B7-2, B7-1 B7-2, OX-40L, 41 BBL and/or ICAM-1 are administered. IL-15 or an IL- 15/IL-15 receptor complex can be administered.

A number of means for inducing cellular responses, both in vitro and in vivo, are known. Lipids are capable of assisting in priming T cells in vivo against various antigens. For example, as described in U.S. Patent No. 5,662,907, palmitic acid residues can be attached to the alpha and epsilon amino groups of a lysine residue and then linked (for example, via one or more linking residues, such as glycine, glycine-glycine, serine, serine-serine, or the like) to an immunogenic peptide or protein. The lipidated peptide can then be injected directly in a micellar form, incorporated in a liposome, or emulsified in an adjuvant. As another example, E. coli lipoproteins, such as tripalmitoyl-S-glycerylcysteinlyseryl-serine can be used to prime tumor specific T cells when covalently attached to an appropriate peptide or protein (see, Deres et al., Nature 342:561, 1989). Further, as the induction of neutralizing antibodies can also be primed with the same molecule conjugated to a protein which displays an appropriate epitope, two compositions can be combined to elicit both humoral and cell-mediated responses where that is deemed desirable.

A pharmaceutical composition, such as a vaccine, including a Tp0751 protein fragment is thus provided. These compositions are used to generate an immune response, such as for immunotherapy.

In one embodiment, the Tp0751 protein fragment is mixed with an adjuvant containing two or more of a stabilizing detergent, a micelle-forming agent, and an oil. Suitable stabilizing detergents, micelle-forming agents, and oils are detailed in U.S. Patent No. 5,585,103; U.S. Patent No. 5,709,860; U.S. Patent No. 5,270,202; and U.S. Patent No. 5,695,770, all of which are incorporated by reference. A stabilizing detergent is any detergent that allows the components of the emulsion to remain as a stable emulsion. Such detergents include

polysorbate, 80 (TWEEN) (Sorbitan-mono-9-octadecenoate-poly(oxy-l,2-ethanediyl;

manufactured by ICI Americas, Wilmington, DE), TWEEN 40™, TWEEN 20™, TWEEN 60™, Zwittergent™ 3-12, TEEPOL HB7™, and SPAN 85™. These detergents are usually provided in an amount of approximately 0.05 to 0.5%, such as at about 0.2%. A micelle forming agent is an agent which is able to stabilize the emulsion formed with the other components such that a micelle-like structure is formed. Such agents generally cause some irritation at the site of injection in order to recruit macrophages to enhance the cellular response. Examples of such agents include polymer surfactants described by BASF Wyandotte

publications, e.g., Schmolka, J. Am. Oil. Chem. Soc. 54: 110, 1977, and Hunter et al., J. Immunol 129: 1244, 1981, PLURONIC™ L62LF, L101, and L64, PEG1000, and TETRONIC™ 1501, 150R1, 701, 901, 1301, and 130R1. The chemical structures of such agents are well known in the art. In one embodiment, the agent is chosen to have a hydrophile-lipophile balance (HLB) of between 0 and 2, as defined by Hunter and Bennett, J. Immun. 133:3167, 1984. The agent can be provided in an effective amount, for example between 0.5 and 10%, or in an amount between 1.25 and 5%. The oil included in the composition is chosen to promote the retention of the antigen in oil-in-water emulsion, such as to provide a vehicle for the desired antigen, and preferably has a melting temperature of less than 65°C such that emulsion is formed either at room temperature (about 20°C to 25°C), or once the temperature of the emulsion is brought down to room temperature. Examples of such oils include squalene, Squalane, EICOSANE™,

tetratetracontane, glycerol, and peanut oil or other vegetable oils. In one specific, non-limiting example, the oil is provided in an amount between 1 and 10%, or between 2.5 and 5%. The oil should be both biodegradable and biocompatible so that the body can break down the oil over time, and so that no adverse affects, such as granulomas, are evident upon use of the oil.

In one embodiment, the adjuvant is a mixture of stabilizing detergents, micelle-forming agent, and oil available under the name PROVAX® (IDEC Pharmaceuticals, San Diego, CA). In other embodiments, the Tp0751 fragment is included in a liposome.

Adjuvants can also be administered with the Tp0751 fragment. An adjuvant can be any immuno stimulatory molecule, such as a cytokine, immunostimulatory nucleic acid, or a biological adjuvant (see above). The adjuvant can be chitosan. Chitosan is a linear

polysaccharide formed from repeating beta (1-4 linked) N-acetyl-D-glucosamine and D- glucosamine units, and is derived from the partial deacetylation of chitin obtained from the shells of crustaceans. Chitosan can be made commercially by a heterogeneous alkaline hydrolysis of chitin to give a product which possesses a random distribution of remaining acetyl moieties. The properties of chitosans depend upon inter alia the degree of deacetylation, and the molecular weight. Most commercially available chitosans contain a population of chitosan molecules of varying molecular weights and varying concentrations of the component N-acetyl- D-glucosamine and D-glucosamine groups. The immunological properties of chitosans are known to be linked to the ratio between the N-acetyl-D-glucosamine and D-glucosamine groups. The efficacy of chitosans as adjuvants depends to a considerable extent on the extent of the level of deacetylation. Thus, in some embodiments, the chitosan is at least 80% deacetylated, see U.S. Patent No. 6,534,065, which is incorporated herein by reference.

Controlled release parenteral formulations can be made as implants, oily injections, or as particulate systems. For a broad overview of protein delivery systems, see Banga, Therapeutic Peptides and Proteins: Formulation, Processing, and Delivery Systems, Technomic Publishing Company, Inc., Lancaster, PA, 1995. Particulate systems include microspheres, microparticles, microcapsules, nanocapsules, nanospheres, and nanoparticles. Microcapsules contain the therapeutic protein as a central core. In microspheres, the therapeutic agent is dispersed throughout the particle. Particles, microspheres, and microcapsules smaller than about 1 μιη are generally referred to as nanoparticles, nanospheres, and nanocapsules, respectively. Capillaries have a diameter of approximately 5 μιη so that only nanoparticles are administered

intravenously. Microparticles are typically around 100 μιη in diameter and are administered subcutaneously or intramuscularly (see Kreuter, Colloidal Drug Delivery Systems, J. Kreuter, ed., Marcel Dekker, Inc., New York, NY, pp. 219-342, 1994; Tice & Tabibi, Treatise on Controlled Drug Delivery, A. Kydonieus, ed., Marcel Dekker, Inc. New York, NY, pp. 315-339, 1992).

Polymers can be used for ion-controlled release. Various degradable and nondegradable polymeric matrices for use in controlled drug delivery are known in the art (Langer, Accounts Chem. Res. 26:53 ', 1993). For example, the block copolymer, polaxamer 407 exists as a viscous yet mobile liquid at low temperatures but forms a semisolid gel at body temperature. It has shown to be an effective vehicle for formulation and sustained delivery of recombinant interleukin-2 and urease (Johnston et al., Pharm. Res. 9:425, 1992; and Pec, . Parent. Sci. Tech. 44(2):58, 1990). Alternatively, hydroxy apatite has been used as a microcarrier for controlled release of proteins (Ijntema et al., Int. J. Pharm. 112:215, 1994). In yet another aspect, liposomes are used for controlled release as well as drug targeting of the lipid-capsulated drug (Betageri et al., Liposome Drug Delivery Systems, Technomic Publishing Co., Inc., Lancaster, PA, 1993). Numerous additional systems for controlled delivery of therapeutic proteins are known (e.g., U.S. Patent No. 5,055,303; U.S. Patent No. 5,188,837; U.S. Patent No. 4,235,871; U.S. Patent No. 4,501,728; U.S. Patent No. 4,837,028; U.S. Patent No. 4,957,735; and U.S. Patent No. 5,019,369; U.S. Patent No. 5,055,303; U.S. Patent No. 5,514,670; U.S. Patent No. 5,413,797; U.S. Patent No. 5,268,164; U.S. Patent No. 5,004,697; U.S. Patent No. 4,902,505; U.S. Patent No. 5,506,206; U.S. Patent No. 5,271,961; U.S. Patent No. 5,254,342; and U.S. Patent No. 5,534,496).

In another embodiment, the composition includes a nucleic acid encoding a Tp0751 protein fragment. A therapeutically effective amount of the polynucleotide encoding the Tp0751 protein fragment can be administered to a subject in order to generate an immune response. In one specific, non-limiting example, a therapeutically effective amount of the polynucleotide encoding the Tp0751 protein fragment is administered to a subject to treat or prevent the effects of T. pallidum infection.

Optionally, a non-pox non-yeast vector is administered that encodes one or more immuno stimulatory or costimulatory molecules, such as IL-2, IL-6, IL-12, IL-15, LFA (for example, LFA-1, LFA-2 and/or LFA-3), CD72, RANTES, G-CSF, GM-CSF, TNF-a, IFN-γ,

ICAM-1, , B7-1, B7-2, other B7 related molecules,OX-40L or 41 BBL, or combinations of these molecules (see, for example, Salgaller et al., 1998, J. Surg. Oncol. 68(2): 122-38; Lotze et al., 2000, Cancer J Sci. Am. 6(Suppl l):S61-6; Cao et al., 1998, Stem Cells 16(Suppl l):251-60; Kuiper et al., 2000, Adv. Exp. Med. Biol. 465:381-90). In some examples, the vector can encode IL-2, RANTES, GM-CSF, TNF-a, IFN-γ, G-CSF, LFA-3, CD72, B7-1, B7-2, B7-1 B7- 2, OX-40L, 41 BBL and/or ICAM-1. In various embodiments, the nucleic acid encoding the biological adjuvant can be cloned into same vector as the Tp0751 fragment coding sequence, or the nucleic acid can be cloned into one or more separate vectors for co-administration. In addition, nonspecific immunomodulating factors such as Bacillus Cahnette-Guerin (BCG) and levamisole can be co-administered.

One approach to administration of nucleic acids is direct immunization with plasmid DNA, such as with a mammalian expression plasmid. As described above, the nucleotide sequence encoding a Tp0751 fragment or polypeptide can be placed under the control of a promoter to increase expression of the molecule.

Immunization by nucleic acid constructs is well known in the art and taught, for example, in U.S. Patent No. 5,643,578 (which describes methods of immunizing vertebrates by introducing DNA encoding a desired antigen to elicit a cell-mediated or a humoral response), and U.S. Patent No. 5,593,972 and U.S. Patent No. 5,817,637 (which describe operably linking a nucleic acid sequence encoding an antigen to regulatory sequences enabling expression). U.S.

Patent No. 5,880,103 describes several methods of delivery of nucleic acids encoding immunogenic peptides or other antigens to an organism. The methods include liposomal delivery of the nucleic acids (or of the synthetic peptides themselves), and immune- stimulating constructs, or ISCOMS™, negatively charged cage-like structures of 30-40 nm in size formed spontaneously on mixing cholesterol and Quil A™ (saponin). Protective immunity has been generated in a variety of experimental models of infection, including toxoplasmosis and Epstein-

Barr virus-induced tumors, using ISCOMS™ as the delivery vehicle for antigens (Mowat and

Donachie, Immunol. Today 12:383, 1991). Doses of antigen as low as 1 μg encapsulated in

ISCOMS™ have been found to produce Class I mediated CTL responses (Takahashi et al.,

Nature 344:873, 1990). In other embodiments, the nucleic acid can be loaded onto gold microspheres by standard methods and introduced into the skin by a device such as Bio-Rad's

HELIOS™ Gene Gun. The nucleic acids can be "naked," consisting of plasmids under control of a strong promoter. Typically, the DNA is injected into muscle, although it can also be injected directly into other sites, including tissues in proximity to metastases. Dosages for injection are usually around 0.5 g/kg to about 50 mg/kg, and typically are about 0.005 mg/kg to about 5 mg/kg (see, for example, U.S. Patent No. 5,589,466). In another approach to using nucleic acids for immunization, a Tp0751 fragment can also be expressed by attenuated host cells or non-pox non-yeast viral vectors. These vectors and host cells are disclosed above. Suitable non-pox non-yeast viral vector, include an adenovirus, an alphvirus, a lentivirus, a measles virus or a poliovirus vector. Suitable host cell include an attenuated bacterium, such as Listeria or Salmonella host cells.

When a non-pox viral vector is utilized, it is desirable to provide the recipient with a dosage of each recombinant virus in the composition in the range of from about 10 ⁵ to about 10 ¹⁰ plaque forming units/mg mammal, although a lower or higher dose can be administered. The composition of recombinant viral vectors can be introduced into a mammal either prior to any evidence of a cancer, or to mediate regression of the disease in a mammal afflicted with the cancer. Examples of methods for administering the composition into mammals include, but are not limited to, exposure of cells to the recombinant virus ex vivo, or injection of the composition into the affected tissue or intravenous, subcutaneous, intradermal or intramuscular

administration of the virus. Alternatively the recombinant viral vector or combination of recombinant viral vectors may be administered locally by direct injection into the cancerous lesion in a pharmaceutically acceptable carrier. Generally, the quantity of recombinant non-pox viral vector, carrying the nucleic acid sequence of a Tp0751 fragment to be administered is based on the titer of virus particles. An exemplary range of the immunogen to be administered is 10 ⁵ to 10 ¹⁰ virus particles per mammal, such as a human.

In the embodiment where a combination of a first recombinant viral vector carrying a nucleic acid sequence of a Tp0751 fragment and a second recombinant viral vector carrying the nucleic acid sequence of one or more costimulatory or immunostimulatory molecules is used, the mammal can be immunized with different ratios of the first and second recombinant viral vector. In one embodiment the ratio of the first vector to the second vector is about 1: 1, or about 1:3, or about 1:5. Optimal ratios of the first vector to the second vector may easily be titered using the methods known in the art (see, for example, U.S. Patent No. 6,893,869, incorporated herein by reference). Simultaneous production of an immunostimulatory molecule and the Tp0751 fragment enhances the generation of specific effectors. Without being bound by theory, dependent upon the specific immunostimulatory molecules, different mechanisms might be responsible for the enhanced immunogenicity: augmentation of help signal (IL-2), recruitment of professional APC (GM-CSF), increase in CTL frequency (IL-2), effect on antigen processing pathway and MHC expression (IFNy and TNFa) and the like. For example, IL-2, IL-6, IL-15, interferon, or a nucleic acid encoding these molecules, can be administered in conjunction with a Tp0751 fragment, or a nucleic acid encoding a Tp0751 fragment. The co-expression of a Tp0751 fragment together with at least one immunostimulatory molecule can be effective in an animal model to show immunogenic effects.

Single or multiple administrations of the compositions are administered depending on the dosage and frequency as required and tolerated by the subject. In one embodiment, the dosage is administered once as a bolus, but in another embodiment can be applied periodically until a therapeutic result is achieved. Generally, the dose is sufficient to induce an immune response to Tp0751, to treat or ameliorate symptoms or signs of disease, without producing unacceptable toxicity to the subject. Systemic or local administration can be utilized.

Kits Containing Tp0751 Protein Fragments

Isolated Tp0751 protein fragments (e.g., SEQ ID NO: 3 or 7 with a tag, or a protein having at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity to any of SEQ ID NO: 3 or 7) or fragments that include a mutated active site (e.g., any of SEQ ID NOs: 4-6 or 8-10, or sequences having at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity to any of SEQ ID NOs: 4-6 or 8- 10 that retain the mutated active site) can be part of a kit. Such kits can include the Tp0751 protein fragment (or nucleic acid encoding such), as well as other materials, such as instructions for use in using the agent as an immunogen (e.g., vaccine) against T. pallidum. Such kits can also include other agents, such as an adjuvant, syringes, and the like.

EXAMPLE 1

Generation and Purification Tp0751 Fragments

The example describes methods used to generate and purify fragments of Tp0751 (amino acids 54-173, 24-237 and 25-237).

Tp0751 fragments (amino acids 24-237 (SEQ ID NO: 7) or 25-237 (SEQ ID NO: 3)) were separately expressed as a recombinant protein with a 6xHis tag using pDEST17 (Life Technologies Gateway system) in E. coli B121-AI cells (this results in a 6-His tag at the N- terminus of Tp0751). The cells were grown in LB medium. The Tp0751 protein fragments were induced and expressed while the bacteria were in stationary phase. Briefly, the cells were grown to an OD600 of 1.9 at 37°C, cooled to 16°C for 1 h, induced with 0.2% L-Arabinose, and grown for 16 h at 16°C. The cells were lysed and the soluble portion of the lysate was purified using affinity chromatography. Briefly, cells were pelleted, protease inhibitor cocktail added, and cells resuspended in 20 mM HEPES/500 mM NaCl/ 20 mM imidazole/1% glycerol/ 25 mM CaC12/ 10 μΜ ZnC12 pH 7.0 before flash freezing. Resuspended cells were stored in -80°C, thawed on ice, and lysed using a detergent (CHAPS) and sonication. The lysate was centrifuged and only the soluble fraction of the lysate was carried forward.

The fractions containing the Tp0751 fragment, as determined by SDS-PAGE, were then purified a second time and buffer exchanged using size exclusion chromatography. Briefly, the lysate was purified using immobilized metal affinity chromatography (IMAC) (on the Akta Prime FPLC fitted with a HisTrap FF (GE) 1 mL column running an imidazole gradient elution. Fractions were pooled and concentrated to 5 mL using a 10,000 Dalton molecular weight cut off centrifugal concentrator. The Affinity Binding/Wash Buffer was 20 mM HEPES/500 mM NaCl/20 mM imidazole/1% glycerol/ 25 mM CaC12/ 10 μΜ ZnC12 pH 7.0 and the Affinity Elution Buffer used was 20 mM HEPES/500 mM NaCl/500 mM imidazole/1% glycerol/ 25 mM CaC12/ 10 μΜ ZnC12 pH 7.0. The protein was purified a second time using size exclusion chromatography on the Akta Prime FPLC fitted with a Hiload Superdex 75 16/60 column.

Fractions were collected, pooled, flash frozen, and stored at -80°C. The steps from lysis to the final freezing are completed within one day. This protocol resulted in a >99% pure, soluble protein. The Size Exclusion Buffer used was 20 mM HEPES/150 mM NaCl/ 25 mM

CaC12/l%glycerol pH 7.0.

One skilled in the art will appreciate that other buffers can be used, such as:

Affinity binding/resuspension/wash buffer: 20 mM HEPES, 500 mM NaCl, 20 mM imidazole, pH 7.0

Affinity elution buffer: 20 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH

7.0

Size exclusion chromatography buffer: 20 mM HEPES, 150 mM NaCl, pH 7.0

EXAMPLE 2

Use of Tp0751 Fragments as an Immunogen

The example describes methods used to immunize rabbits with fragments of Tp0751 (24- 237 (SEQ ID NO: 7) and 25-237 (SEQ ID NO: 3)).

An overview of the methods used to immunize (Example 2) and challenge rabbits (Example 3) is shown in FIG. 1. Briefly, two unimmunized control rabbits and three rabbits that had each received three immunizations with wild-type Tp0751 (SEQ ID NO: 7) (125 μg, 1: 1 with TiterMax® Gold adjuvant) were challenged with 10 ⁶ T. pallidum at each of 10 sites. These rabbits (termed the donor rabbits) were euthanized at day 14 post-challenge, the lymph nodes were collected and naive rabbits were infected with these lymph nodes (these are the recipient rabbits). This is called the rabbit infectivity test (RET) (Example 4). For an RIT, one naive recipient rabbit receives lymph nodes from one donor rabbit. If the naive RIT rabbit becomes infected, it is concluded that the T. pallidum disseminated to the lymph nodes in the donor rabbit. The organ and tissue burden of T. pallidum in the donor rabbits (both control unimmunized and immunized) was determined using quantitative PCR.

Rabbits (New Zealand White specific pathogen free, Males, 3 months old, 2.5 to 3 kg) were shaved across the shoulders and each injection site was cleansed with rubbing alcohol. Rabbits were immunized with Tp0751 fragment amino acids 24-237 (SEQ ID NO: 7) containing an N-terminal 6-His tag (see Example 1) as follows. A water in oil emulsion was made of 0.52 mg/mL of the Tp0751 antigen in aqueous buffer into TiterMax Gold (Sigma cat #T2684 lot SLBB0432V) adjuvant in a ratio of 1: 1 following the manufacture's instructions. Three rabbits were immunized with four subcutaneous (SC) and two intramuscular (IM) injections of the emulsion. Two SC injections of 0.1 mL were done on each shoulder, total of 4 per rabbit per immunization, avoiding previous injection sites. One IM injection of 0.04 mL was done into each quadriceps muscle, for a total of 2 injections per rabbit per immunization.

One week later the rabbits were bled and titered. Three weeks after the first

immunization, the rabbits were boosted, bled, and titered in the same manner. A final boost was done six weeks after the initial immunization. Blood collection was done 7 days after the third immunization. A final blood collection was done 18 days after final immunization (day 67). An intradermal (ID) challenge was performed 25 days after final immunization. FIG. 2 shows the resulting antibody titers (these were determined via ELISA using the recombinant Tp0751 protein and serum collected from the immunized rabbits.). Thus, the Tp0751 fragment aa 24- 237 works well as an antigen.

EXAMPLE 3

Tp0751 Fragments Protect Rabbits from Subsequent T. pallidum Challenge

The example describes methods used to challenge rabbits with live T. pallidum who were previously immunized with fragments of Tp0751 (see Example 2).

Vaccinated rabbits and unvaccinated rabbits were challenged with live T. pallidum bacteria to determine how the immunizations affected disease progression and bacterial dissemination. Treponema pallidum were propagated using methods known in the art (see Lukehart and Marra. 2007. Isolation and Laboratory Maintenance of Treponema pallidum. Current Protocols in Microbiology, 12A.1.-12A.1.18 with the exception that testicular extractions were done in an anaerobic environment). The ID challenge was performed as follows. 25 days after the final immunization the backs of rabbits were shaved and cleansed with 70% ethanol and 0.1 niL of Ιχΐθ' Treponema pallidum/mL in 0.9% saline was intradermally injected, in each of 10 spots. Chancres were monitored and photographed daily for erythema, induration, ulceration, and measured for length, width, and height. Using a syringe fitted with a 26G needle, the center of the chancres was aspirated, and the aspirate examined using a darkfield microscope.

The immunized rabbits developed lesions upon challenge, as did the control rabbits upon challenge. At the point the rabbits were euthanized (day 14 post-challenge) there were qualitative and quantitative differences between the two groups as shown in Table 2.

Table 2: Effect of immunization on chancres following subsequent T. pallidum infection

Thus, immunization with the Tp0751 fragment partially protects against lesion ulceration and results in smaller chancres. As shown in Table 2 and FIGS. 3A-3B, the immunized rabbits had delayed lesion development compared to the control unimmunized rabbits. All three immunized rabbits had approximately equal, high titers to Tp0751. Ulceration has a very crusty quality and is marked by a brick red color. An ulcerated lesion has a crater- like morphology (raised edge with dipped center). 100% of the lesions in the control rabbits ulcerated compared to about 50% of the lesions in the immunized rabbits (FIG. 3A). 72% of the control rabbit lesions were darkfield positive (i.e., had treponemes present) at day 10 post-challenge compared to 13% of the immunized rabbit lesions. For the darkfield positive lesions from the control rabbits, there were a large number of motile treponemes present, while for the darkfield positive lesions from the immunized rabbits there were only a couple of treponemes present in 10 fields and they were non-motile. In addition, rabbits challenged ID with Treponema pallidum had VDRL (Venereal Disease Research Laboratory) seroconversion by day 15, indicating all rabbits had robust infection. EXAMPLE 4

Rabbit Infectivity Test (RIT)

This example describes the RIT, to determine if T. pallidum infection disseminated to lymph nodes in immunized rabbits upon subsequent challenge with virulent T. pallidum.

The RIT experiment was performed as follows. Popliteal lymph nodes were removed from ID challenge rabbits, extracted in saline, and injected into the testes of naive rabbits according to Lukehart and Marra {Current Protocols in Microbiology, 12A.1.-12A.1.18, 2007). RIT rabbits were monitored by serology (VDRL and FTA-Abs) and palpitation of the testes to check for infection. Testicular aspirates were also taken to confirm infection. Thus, as serological markers of infection the VDRL (test for non-treponemal antibodies) and FTA-Abs (test for treponemal antibodies) were used. Physical signs of infection were monitored by looking for orchitis (swelling of the testes due to infection) and by darkfield microscopy (visualization of testicular aspirates).

A summary is provided in Table 3.

Table 3: Results of RIT

The recipient RIT rabbits receiving lymph nodes from the control unimmunized donor rabbits developed orchitis (swelling of the testes associated with T. pallidum infection) at day 37 post transfer. These rabbits were euthanized on day 39 (rabbit number 48) and day 42 (rabbit number 47) post transfer. Darkfield analysis of testes aspirates on day 38 post-transfer showed both rabbits had visible treponemes. Table 4 shows the seroconversion status of recipient RIT rabbits from control unimmunized donor rabbits.

Table 4

The recipient RIT rabbits receiving lymph nodes from the immunized donor rabbits did not develop orchitis. One of three of these animals seroconverted (rabbit#43). This rabbit never developed orchitis. Rabbit 43 was euthanized on day 134 due to an unrelated suspected gastrointestinal problem (possibly due to eating too many treats). Table 5 shows the

seroconversion status of recipient RIT rabbits from immunized donor rabbits.

Table 5

In summary, Tp0751 immunization inhibits treponemal dissemination to lymph nodes in rabbits upon subsequent challenge with virulent T. pallidum. 2/3 of RIT recipient rabbits that received lymph nodes from Tp0751 -immunized donor rabbits show no signs of infection through the 185 days of the experiment (no seroconversion, no orchitis, darkfield negative). This shows T. pallidum had not disseminated to the lymph nodes in these two immunized animals. In addition, 2/2 RIT recipient rabbits that received lymph nodes from unimmunized control donor rabbits developed infection by day 17 post-transfer (both rabbits seroconverted by day 17 post-transfer, developed robust orchitis at day 37, and were darkfield positive at the time of euthanasia on days 39 and 42).

EXAMPLE 5

Tp0751 Inactive Fragment Has No Effects

The example describes methods used to assess the effects of a Tp0751 fragment with an inactive active site in vivo.

Proteolytically active Tp0751 (Example 1, SEQ ID NO: 7), but not an inactive form (SEQ ID NO: 8), caused a reaction in a dose dependent manner.

Injection of 25 μg of wild type Tp0751 (aa 24-237, SEQ ID NO: 7) into rabbits (n=2) in the absence of adjuvant caused a "chancre" to appear at the injection site at day 2 post-injection that disappeared by day 9. To avoid this undesired consequence, the Tp0751 fragment was mutated at its active site to AEXXH. In contrast, 25 μg of the proteolytically inactive mutant of Tp0751 (SEQ ID NO: 8) had no reaction.

Similarly, rabbits injected with 25 μg of wild type Tp0751 (aa 24-237, SEQ ID NO: 7) + TiterMax Gold adjuvant, but not 25 μg of the proteolytically inactive mutant of Tp0751 (SEQ ID NO: 8) + TiterMax® Gold adjuvant, or TiterMax® Gold adjuvant alone, had ulcerated lesions by day 5 that were similar in gross appearance to chancres arising in rabbits

intradermally challenged with 10 ⁶ T. pallidum (day 14 post-challenge). Thus Tp0751 fragments can be mutated to inactive their active site to reduce the appearance of a chancre-like lesion at the injection site.

EXAMPLE 6

Generation and Purification Tp0751 Fragments with Mutated Activation Site

The example describes methods used to generate and purify fragments of Tp0751 (amino acids 24-237 and 25-237) containing a mutated activation site, such as those shown in SEQ ID NOS: 4-6 and 8-10. Such proteins can include a 6X-His tag.

SEQ ID NOS: 4-6 and 8-10 with the active site mutated were generated using site- directed mutagenesis. The proteins were purified as described in Example 1.

While this disclosure has been described with an emphasis upon particular embodiments, it will be obvious to those of ordinary skill in the art that variations of the particular

embodiments may be used and it is intended that the disclosure may be practiced otherwise than as specifically described herein. Accordingly, this disclosure includes all modifications encompassed within the spirit and scope of the disclosure as defined by the following claims.