Title:
INDUCED TOTIPOTENT STEM CELLS AND PREPARATION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2023/025302
Kind Code:
A1
Abstract:
Provided are a combination of small molecule reprogramming reagents used to induce totipotent stem cells, a method for preparing induced totipotent stem cells, and induced totipotent stem cells.
Inventors:
DING SHENG (CN)
LIU KANG (CN)
HU YANYAN (CN)
YANG YUANYUAN (CN)
TAN PENGCHENG (CN)
MA TIANHUA (CN)
LIU KANG (CN)
HU YANYAN (CN)
YANG YUANYUAN (CN)
TAN PENGCHENG (CN)
MA TIANHUA (CN)
Application Number:
PCT/CN2022/115235
Publication Date:
March 02, 2023
Filing Date:
August 26, 2022
Export Citation:
Assignee:
UNIV TSINGHUA (CN)
International Classes:
C12N5/071; C12N5/078; C12N5/0783
Domestic Patent References:
| WO2020200071A1 | 2020-10-08 |
Foreign References:
| CN108934168A | 2018-12-04 | |||
| US20170114323A1 | 2017-04-27 | |||
| US20160060593A1 | 2016-03-03 | |||
| CN110832069A | 2020-02-21 | |||
| CN102333863A | 2012-01-25 | |||
| CN106554936A | 2017-04-05 |
Other References:
HU YANYAN, YANG YUANYUAN, TAN PENGCHENG, ZHANG YUXIA, HAN MENGXIA, YU JIAWEI, ZHANG XIN, JIA ZERAN, WANG DAN, LI YINQING, MA TIANH: "Induction of mouse totipotent stem cells by a defined chemical cocktail", NATURE, NATURE PUBLISHING GROUP UK, LONDON, London, XP093038890, ISSN: 0028-0836, DOI: 10.1038/s41586-022-04967-9
SHEN HUI; YANG MIN; LI SHIYU; ZHANG JING; PENG BING; WANG CHUNHUI; CHANG ZAI; ONG JENNIE; DU PENG: "Mouse totipotent stem cells captured and maintained through spliceosomal repression", CELL, ELSEVIER, AMSTERDAM NL, vol. 184, no. 11, 14 May 2021 (2021-05-14), Amsterdam NL , pages 2843, XP086581571, ISSN: 0092-8674, DOI: 10.1016/j.cell.2021.04.020
SHEN HYANG MLI S ET AL.: "Mouse totipotent stem cells captured and maintained through spliceosomal repression [J", CELL, vol. 184, no. 11, 2021, pages 2843 - 2859, XP086581571, DOI: 10.1016/j.cell.2021.04.020
SAMBROOK ET AL.: "molecular clocking: a laboratory manual", 2001, article "Hematopoietic Stem Cell Protocols"
MANIATIS ET AL.: "Molecular Cloning: A Laboratory Manual", 1982
AUSUBEL ET AL.: "Current Protocols in Molecular Biology", July 2008, JOHN WILEY AND SONS
GLOVER: "Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular Biology", vol. 1, 2, 1985, GREENE PUB. ASSOCIATES AND WILEY-INTERSCIENCE
WEIS ET AL., TIG, vol. 8, 1992, pages 263 - 64
GUTHRIEFINK: "Guide to Yeast Genetics and Molecular Biology", 1991, ACADEMIC PRESS
PERBAL: "A Practical Guide to Molecular Cloning", 1984
"Handbook Of Experimental Immunology", vol. I-IV, 1986, IRL PRESS
FIRE ET AL.: "RNA Interference Technology: From Basic Science to Drug Development", 2005, CAMBRIDGE UNIVERSITY PRESS
ENGELKE: "RNA Interference (RNAi): The Nuts & Bolts of siRNA Technology", 2003, DNA PRESS
NEURAL STEM CELLS: METHODS AND PROTOCOLS: "Methods in Molecular Biology", 2008, HUMAN PRESS, article "RNAInterference, Editing, and Modification: Methods and Protocols"
SOHAIL: "Gene Silencing by RNA Interference: Technology and Application", 2004, CRC
CLARKESANSEAU: "Nuts & Bolts series", 2006, DNA PRESS, article "microRNA: Biology, Function & Expression"
"Immunochemical Methods In Cell And Molecular Biology", 1987, COLD SPRING HARBOR LABORATORY
HARLOWLANE: "Antibodies", 1998, COLD SPRING HARBOR LABORATORY PRESS
RIOTT: "Essential Immunology", 1988, SCIENTIFIC PUBLICATIONS
"Embryonic Stem Cell Protocols", vol. II, 2006, article "Isolation and Characterization (Methods in Molecular Biology"
"Human Embryonic Stem Cell Protocols (Methods in Molecular Biology", 2006
HOGAN ET AL.: "Methods of Manipulating the Mouse Embryo", 2002
"The Zebrafish book", 2000, UNIV. OF OREGON PRESS, article "A guide for the laboratory use of zebrafish (Danio rerio"
SCHENA ET AL., SCIENCE, vol. 270, 1995, pages 467 - 70
POSFAI, E ET AL.: "Evaluating totipotency using criteria of increasing stringency", NATURE CELL BIOLOGY, vol. 23, 2021, pages 49 - 60, XP037330787, DOI: 10.1038/s41556-020-00609-2
YANG, Y ET AL.: "Derivation of pluripotent stem cells with in vivo intra-embryonic and extra-embryonic potency", CELL, vol. 169, 2017, pages 243 - 257
SHEN, H ET AL.: "Mouse totipotent stem cells captured and maintained through spliceosomal repression", CELL, DOI:10.1016/J.CELL.2021.04.020, 2021
DENG, Q.RAMSKOLD, D.REINIUS, B.SANDBERG, R.: "Single-cell RNA-seq reveals dynamic, random monoallelic gene expression in mammalian cells", SCIENCE, vol. 343, 2014, pages 193 - 196
YING, Q. L. ET AL.: "The ground state of embryonic stem cell self-renewal", NATURE, vol. 453, 2008, pages 519 - 523, XP055033153, DOI: 10.1038/nature06968
LI, R ET AL.: "Generation of Blastocyst-like Structures from Mouse Embryonic and Adult Cell Cultures", CELL, vol. 179, 2019, pages 687 - 702
SHEN HUI; YANG MIN; LI SHIYU; ZHANG JING; PENG BING; WANG CHUNHUI; CHANG ZAI; ONG JENNIE; DU PENG: "Mouse totipotent stem cells captured and maintained through spliceosomal repression", CELL, ELSEVIER, AMSTERDAM NL, vol. 184, no. 11, 14 May 2021 (2021-05-14), Amsterdam NL , pages 2843, XP086581571, ISSN: 0092-8674, DOI: 10.1016/j.cell.2021.04.020
SHEN HYANG MLI S ET AL.: "Mouse totipotent stem cells captured and maintained through spliceosomal repression [J", CELL, vol. 184, no. 11, 2021, pages 2843 - 2859, XP086581571, DOI: 10.1016/j.cell.2021.04.020
SAMBROOK ET AL.: "molecular clocking: a laboratory manual", 2001, article "Hematopoietic Stem Cell Protocols"
MANIATIS ET AL.: "Molecular Cloning: A Laboratory Manual", 1982
AUSUBEL ET AL.: "Current Protocols in Molecular Biology", July 2008, JOHN WILEY AND SONS
GLOVER: "Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular Biology", vol. 1, 2, 1985, GREENE PUB. ASSOCIATES AND WILEY-INTERSCIENCE
WEIS ET AL., TIG, vol. 8, 1992, pages 263 - 64
GUTHRIEFINK: "Guide to Yeast Genetics and Molecular Biology", 1991, ACADEMIC PRESS
PERBAL: "A Practical Guide to Molecular Cloning", 1984
"Handbook Of Experimental Immunology", vol. I-IV, 1986, IRL PRESS
FIRE ET AL.: "RNA Interference Technology: From Basic Science to Drug Development", 2005, CAMBRIDGE UNIVERSITY PRESS
ENGELKE: "RNA Interference (RNAi): The Nuts & Bolts of siRNA Technology", 2003, DNA PRESS
NEURAL STEM CELLS: METHODS AND PROTOCOLS: "Methods in Molecular Biology", 2008, HUMAN PRESS, article "RNAInterference, Editing, and Modification: Methods and Protocols"
SOHAIL: "Gene Silencing by RNA Interference: Technology and Application", 2004, CRC
CLARKESANSEAU: "Nuts & Bolts series", 2006, DNA PRESS, article "microRNA: Biology, Function & Expression"
"Immunochemical Methods In Cell And Molecular Biology", 1987, COLD SPRING HARBOR LABORATORY
HARLOWLANE: "Antibodies", 1998, COLD SPRING HARBOR LABORATORY PRESS
RIOTT: "Essential Immunology", 1988, SCIENTIFIC PUBLICATIONS
"Embryonic Stem Cell Protocols", vol. II, 2006, article "Isolation and Characterization (Methods in Molecular Biology"
"Human Embryonic Stem Cell Protocols (Methods in Molecular Biology", 2006
HOGAN ET AL.: "Methods of Manipulating the Mouse Embryo", 2002
"The Zebrafish book", 2000, UNIV. OF OREGON PRESS, article "A guide for the laboratory use of zebrafish (Danio rerio"
SCHENA ET AL., SCIENCE, vol. 270, 1995, pages 467 - 70
POSFAI, E ET AL.: "Evaluating totipotency using criteria of increasing stringency", NATURE CELL BIOLOGY, vol. 23, 2021, pages 49 - 60, XP037330787, DOI: 10.1038/s41556-020-00609-2
YANG, Y ET AL.: "Derivation of pluripotent stem cells with in vivo intra-embryonic and extra-embryonic potency", CELL, vol. 169, 2017, pages 243 - 257
SHEN, H ET AL.: "Mouse totipotent stem cells captured and maintained through spliceosomal repression", CELL, DOI:10.1016/J.CELL.2021.04.020, 2021
DENG, Q.RAMSKOLD, D.REINIUS, B.SANDBERG, R.: "Single-cell RNA-seq reveals dynamic, random monoallelic gene expression in mammalian cells", SCIENCE, vol. 343, 2014, pages 193 - 196
YING, Q. L. ET AL.: "The ground state of embryonic stem cell self-renewal", NATURE, vol. 453, 2008, pages 519 - 523, XP055033153, DOI: 10.1038/nature06968
LI, R ET AL.: "Generation of Blastocyst-like Structures from Mouse Embryonic and Adult Cell Cultures", CELL, vol. 179, 2019, pages 687 - 702
Attorney, Agent or Firm:
KING & WOOD MALLESONS (CN)
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