Title:
INTELLIGENT CONVERSION DOUBLE-STIMULI-RESPONSIVE PROBE CHELATED WITH METAL IONS, AND PREPARATION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/098007
Kind Code:
A1
Abstract:
An intelligent conversion double-stimuli-responsive probe chelated with metal ions, and a preparation method therefor and the use thereof. The probe can be self-assembled into large nanoparticles in a water buffer solution, and the large nanoparticles are then converted into small nanoparticles under the action of LAP and GSH, so as to enhance the accumulation thereof in a tumor and the deep tissue penetration, thereby improving near-infrared imaging of the tumor in vivo. In addition, it can be found for the first time that this LAP/GSH-driven disassembly and size reduction method can remarkably activate the photodynamic effect of a therapeutic drug, so as to achieve effective imaging-guided PDT on liver tumors and reduce the side effects on normal tissues. The chelating probe Ce6-Leu@Mn 2+ can improve the oxygen supply to overcome hypoxia and enhance the generation of ROS under X-ray radiation, thereby performing effective MRI imaging-guided radiotherapy on human liver HepG2 tumors in living mice. Therefore, a size-convertible nanosystem of the chelating probe may provide a powerful technique for improving the drug delivery efficiency, thereby enhancing tumor diagnosis and treatment.
Inventors:
SHI HAIBIN (CN)
WANG ANNA (CN)
WANG ANNA (CN)
Application Number:
PCT/CN2022/097205
Publication Date:
June 08, 2023
Filing Date:
June 06, 2022
Export Citation:
Assignee:
UNIV SOOCHOW (CN)
International Classes:
A61K41/00; A61K47/69; A61K49/10; A61P35/00; B82Y5/00; C07K1/107; C07K5/083
Foreign References:
| CN114149482A | 2022-03-08 | |||
| JP2018199649A | 2018-12-20 |
Other References:
WANG ANNA, FANG JING, YE SHUYUE, MAO QIULIAN, ZHAO YAN, CUI CHAOXIANG, ZHANG YUQI, FENG YALI, LI JIACHEN, HE LEI, QIU LING, SHI HA: "Assembly Transformation Jointly Driven by the LAP Enzyme and GSH Boosting Theranostic Capability for Effective Tumor Therapy", APPLIED MATERIALS & INTERFACES, AMERICAN CHEMICAL SOCIETY, US, vol. 13, no. 50, 22 December 2021 (2021-12-22), US , pages 59787 - 59802, XP093067497, ISSN: 1944-8244, DOI: 10.1021/acsami.1c21062
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CHEN, QIAN ET AL.: "Drug-Induced Self-Assembly of Modified Albumins as Nanotheranostics for Tumor-Targeted Combination Therapy.", ACS NANO., vol. 9, no. 5, 7 May 2015 (2015-05-07), XP055874180, DOI: 10.1021/acsnano.5b00640
GAO YANG, ZHANG LUYUN, LIU YANHONG, SUN SIJIA, YIN ZHIBIN, ZHANG LILI, LI AIGUO, LU GUANGMING, WU AIGUO, ZENG LEYONG: "Ce6/Mn 2+ -chelated polydopamine@black-TiO 2 nanoprobes for enhanced synergistic phototherapy and magnetic resonance imaging in 4T1 breast cancer", NANOSCALE, ROYAL SOCIETY OF CHEMISTRY, UNITED KINGDOM, vol. 12, no. 3, 23 January 2020 (2020-01-23), United Kingdom , pages 1801 - 1810, XP093071377, ISSN: 2040-3364, DOI: 10.1039/C9NR09236F
FENG LIANGZHU, CHENG LIANG, DONG ZILIANG, TAO DANLEI, BARNHART TODD E., CAI WEIBO, CHEN MEIWAN, LIU ZHUANG: "Theranostic Liposomes with Hypoxia-Activated Prodrug to Effectively Destruct Hypoxic Tumors Post-Photodynamic Therapy", ACS NANO, AMERICAN CHEMICAL SOCIETY, US, vol. 11, no. 1, 24 January 2017 (2017-01-24), US , pages 927 - 937, XP055902312, ISSN: 1936-0851, DOI: 10.1021/acsnano.6b07525
HU, DEHONG ET AL.: "Activatable albumin-photosensitizer nanoassemblies for triple-modal imaging and thermal-modulated photodynamic therapy of cancer.", BIOMATERIALS., vol. 93, 31 March 2016 (2016-03-31), XP029522727, DOI: 10.1016/j.biomaterials.2016.03.037
TAN WEIYI, ZHANG QIUXIN, WANG JIAQING, YI MEIHUI, HE HONGJIAN, XU BING: "Enzymatic Assemblies of Thiophosphopeptides Instantly Target Golgi Apparatus and Selectively Kill Cancer Cells**", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, vol. 60, no. 23, 1 June 2021 (2021-06-01), Hoboken, USA, pages 12796 - 12801, XP093071359, ISSN: 1433-7851, DOI: 10.1002/anie.202102601
CHEN, QIAN ET AL.: "Drug-Induced Self-Assembly of Modified Albumins as Nanotheranostics for Tumor-Targeted Combination Therapy.", ACS NANO., vol. 9, no. 5, 7 May 2015 (2015-05-07), XP055874180, DOI: 10.1021/acsnano.5b00640
GAO YANG, ZHANG LUYUN, LIU YANHONG, SUN SIJIA, YIN ZHIBIN, ZHANG LILI, LI AIGUO, LU GUANGMING, WU AIGUO, ZENG LEYONG: "Ce6/Mn 2+ -chelated polydopamine@black-TiO 2 nanoprobes for enhanced synergistic phototherapy and magnetic resonance imaging in 4T1 breast cancer", NANOSCALE, ROYAL SOCIETY OF CHEMISTRY, UNITED KINGDOM, vol. 12, no. 3, 23 January 2020 (2020-01-23), United Kingdom , pages 1801 - 1810, XP093071377, ISSN: 2040-3364, DOI: 10.1039/C9NR09236F
FENG LIANGZHU, CHENG LIANG, DONG ZILIANG, TAO DANLEI, BARNHART TODD E., CAI WEIBO, CHEN MEIWAN, LIU ZHUANG: "Theranostic Liposomes with Hypoxia-Activated Prodrug to Effectively Destruct Hypoxic Tumors Post-Photodynamic Therapy", ACS NANO, AMERICAN CHEMICAL SOCIETY, US, vol. 11, no. 1, 24 January 2017 (2017-01-24), US , pages 927 - 937, XP055902312, ISSN: 1936-0851, DOI: 10.1021/acsnano.6b07525
Attorney, Agent or Firm:
SUZHOU CREATOR PATENT AND TRADEMARK AGENCY, LTD. (CN)
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