Title:
IONIC LIQUIDS BASED ON NON-NATURAL AMINO ACIDS, PREPARATION METHOD THEREFOR, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/046032
Kind Code:
A1
Abstract:
The present invention relates to ionic liquids based on non-natural amino acids, a preparation method therefor, and use thereof, and particularly provides a substance combination for preparing a protein containing non-natural amino acids. The substance combination comprises: (1) one or more aminoacyl-tRNA synthetases, which can bind to mutated tRNAs; (2) one or more mutated tRNAs, in which the anticodon loop is mutated into a complementary sequence of the termination codon; and (3) a variety of ionic liquids based on non-natural amino acids. The substance combination can be used for recombination and expression of the protein containing non-natural amino acids. The ionic liquids based on non-natural amino acids can improve the read-through efficiency of genetic codon expansion systems for premature termination codons (PTCs) and/or the efficiency of inserting the non-natural amino acids.
Inventors:
XIA QING (CN)
SHI YUJIE (CN)
SHI NINGNING (CN)
SHI YUJIE (CN)
SHI NINGNING (CN)
Application Number:
PCT/CN2023/111283
Publication Date:
March 07, 2024
Filing Date:
August 04, 2023
Export Citation:
Assignee:
UNIV BEIJING (CN)
International Classes:
C12N15/113; C12N5/10; C12N9/00; C12N15/85
Foreign References:
| CN103215227A | 2013-07-24 | |||
| CN110835633A | 2020-02-25 | |||
| CN113699124A | 2021-11-26 | |||
| CN115261344A | 2022-11-01 | |||
| US20190062753A1 | 2019-02-28 |
Other References:
CAPARICA RITA, JÚLIO ANA, BABY ANDRÉ, ARAÚJO MARIA, FERNANDES ANA, COSTA JOÃO, SANTOS DE ALMEIDA TÂNIA: "Choline-Amino Acid Ionic Liquids as Green Functional Excipients to Enhance Drug Solubility", PHARMACEUTICS, MDPI AG, CH, vol. 10, no. 4, 1 December 2018 (2018-12-01), CH , pages 288, XP093144925, ISSN: 1999-4923, DOI: 10.3390/pharmaceutics10040288
NINGNING SHI ET AL.: "Restoration of dystrophin expression in mice by suppressing a nonsense mutation through the incorporation of unnatural amino acids", NAT BIOMED ENG., vol. 6, no. 2, 2 August 2021 (2021-08-02), XP037700910, DOI: 10.1038/s41551-021-00774-1
NINGNING SHI ET AL.: "Restoration of dystrophin expression in mice by suppressing a nonsense mutation through the incorporation of unnatural amino acids", NAT BIOMED ENG., vol. 6, no. 2, 2 August 2021 (2021-08-02), XP037700910, DOI: 10.1038/s41551-021-00774-1
Attorney, Agent or Firm:
LONG AN LAW FIRM (CN)
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