Title:
LIVER CELL CULTURE MEMBRANE, DRUG TRANSPORT ABILITY EVALUATION KIT PROVIDED THEREWITH, AND DRUG TRANSPORT ABILITY EVALUATION METHOD
Document Type and Number:
WIPO Patent Application WO/2021/132586
Kind Code:
A1
Abstract:
Provided are a liver cell culture membrane and a drug transport ability evaluation kit that are to be used for in vitro, easily and directly evaluating drug transport ability, i.e., between the migration of an administered drug from the liver into blood vessels followed by whole body migration and the migration of the drug into bile in the bile duct followed by biliary excretion, which is more dominantly induced and to what extent, as one of important factors of distribution, metabolism and excretion. The liver cell culture membrane 14 comprises a membrane-type cell layer 12 which is formed by cells 12a, such as liver cancer cells, cultured in a membrane form on a porous plastic film 13, wherein the cells 12a have such an orientation property that the blood vessel side becomes the bottom side of the membrane-type cell layer 12 while the bile duct side becomes the top side of the membrane-type cell layer 12. The drug transport ability evaluation kit 1 is provided with: a liver cell culture membrane-holding insert container 10 containing the liver cell culture membrane 14, which is disposed on the bottom thereof, and housing a first test solution 15; and a well plate 20 comprising a well 21, into which the insert container is inserted, and housing a second test solution 25 in which the liver cell culture membrane 14 is to be immersed.
Inventors:
OGIHARA TAKUO (JP)
MIZOI KENTA (JP)
MATSUMOTO EIKO (JP)
MIZOI KENTA (JP)
MATSUMOTO EIKO (JP)
Application Number:
PCT/JP2020/048793
Publication Date:
July 01, 2021
Filing Date:
December 25, 2020
Export Citation:
Assignee:
TAKASAKI UNIV OF HEALTH AND WELFARE (JP)
International Classes:
C12M1/34; C12N5/071; C12N5/077; C12Q1/02
Domestic Patent References:
| WO2012032646A1 | 2012-03-15 | |||
| WO2010082385A1 | 2010-07-22 | |||
| WO2007139144A1 | 2007-12-06 | |||
| WO2011024592A1 | 2011-03-03 |
Foreign References:
| JP2016214149A | 2016-12-22 | |||
| JP2007053972A | 2007-03-08 | |||
| JP2007082528A | 2007-04-05 |
Attorney, Agent or Firm:
SHINKYU PARTNERS P.C. (JP)
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