Title:
MALDI SAMPLE PREPARATION METHOD AND SAMPLE PREPARATION DEVICE
Document Type and Number:
WIPO Patent Application WO/2014/163179
Kind Code:
A1
Abstract:
The present invention involves adhering a sample such as a biological tissue section to a conductive glass slide (S1), and then forming a film layer from an appropriate matrix material by using vacuum deposition so as to cover the sample (S2). The crystals in the matrix material in the film layer are extremely fine and highly uniform. The present invention involves next placing the glass slide on which the matrix film layer is formed in a vaporization solvent atmosphere, and impregnating the matrix film layer with the solvent (S3). When the sufficiently impregnated solvent vaporizes, the substance to be measured in the sample is incorporated into the matrix and re-crystallizes. Furthermore, the present invention involves forming a matrix film layer on the surface again by vacuum deposition (S4). The additional matrix film layer absorbs excess laser beam energy during MALDI, and suppresses alteration and the like of the substance to be measured; hence, it is possible to achieve high detection sensitivity while maintaining high spatial resolution.
Inventors:
OGATA KORETSUGU (JP)
TAKAHASHI KAZUTERU (JP)
KUBO AKIKO (JP)
SUEMATSU MAKOTO (JP)
YAMAMOTO TAKUJI (JP)
TAKAHASHI KAZUTERU (JP)
KUBO AKIKO (JP)
SUEMATSU MAKOTO (JP)
YAMAMOTO TAKUJI (JP)
Application Number:
PCT/JP2014/059946
Publication Date:
October 09, 2014
Filing Date:
April 04, 2014
Export Citation:
Assignee:
SHIMADZU CORP (JP)
UNIV KEIO (JP)
UNIV KEIO (JP)
International Classes:
G01N27/64
Foreign References:
| JP2009168448A | 2009-07-30 | |||
| JP2002148157A | 2002-05-22 | |||
| US20100090099A1 | 2010-04-15 | |||
| US20050035284A1 | 2005-02-17 | |||
| US5854486A | 1998-12-29 | |||
| JP2006514738A | 2006-05-11 | |||
| JP2008232842A | 2008-10-02 | |||
| JP2013137294A | 2013-07-11 |
Other References:
ROTH, M J ET AL.: "Thin-Layer Matrix Sublimation with Vapor-Sorption Induced Co-Crystallization for Sensitive and Reproducible SAMDI-TOF MS Alalysis of Protein Biosensors", J. AM. SOC. MASS SPECTROM., vol. 23, no. 10, 31 July 2012 (2012-07-31), pages 1661 - 1669, XP055228007
BOUSCHEN, W ET AL.: "Matrix vapor deposition/ recrystallization and dedicated spray preparation for high-resolution scanning microprobe matrix-assisted laser desorption/ ionization imaging mass spectrometry (SMALDI- MS) of tissue and single cells", RAPID COMMUN. MASS SPECTROM., vol. 24, no. 3, February 2010 (2010-02-01), pages 355 - 364, XP055228010
YANG, J ET AL.: "Matrix Sublimation/ Recrystallization for Imaging Proteins by Mass Spectrometry at High Spatial Resolution", ANAL. CHEM., vol. 83, no. 14, 15 July 2011 (2011-07-15), pages 5728 - 5734, XP055228035
See also references of EP 2975393A4
KIYOSHI OGAWA ET AL.: "Shimadzu Review", vol. 62, 31 March 2006, SHIMADZU CORPORATION, article "Development of Microscopic Mass Spectrometer", pages: 125 - 135
JUNHAI YANG ET AL.: "Matrix Sublimation/Recrystallization for Imaging Proteins by Mass Spectrometry at High Spatial Resolution", ANALYTICAL CHEMISTRY, vol. 83, 2011, pages 5728 - 5734, XP055228035, DOI: doi:10.1021/ac200998a
BOUSCHEN, W ET AL.: "Matrix vapor deposition/ recrystallization and dedicated spray preparation for high-resolution scanning microprobe matrix-assisted laser desorption/ ionization imaging mass spectrometry (SMALDI- MS) of tissue and single cells", RAPID COMMUN. MASS SPECTROM., vol. 24, no. 3, February 2010 (2010-02-01), pages 355 - 364, XP055228010
YANG, J ET AL.: "Matrix Sublimation/ Recrystallization for Imaging Proteins by Mass Spectrometry at High Spatial Resolution", ANAL. CHEM., vol. 83, no. 14, 15 July 2011 (2011-07-15), pages 5728 - 5734, XP055228035
See also references of EP 2975393A4
KIYOSHI OGAWA ET AL.: "Shimadzu Review", vol. 62, 31 March 2006, SHIMADZU CORPORATION, article "Development of Microscopic Mass Spectrometer", pages: 125 - 135
JUNHAI YANG ET AL.: "Matrix Sublimation/Recrystallization for Imaging Proteins by Mass Spectrometry at High Spatial Resolution", ANALYTICAL CHEMISTRY, vol. 83, 2011, pages 5728 - 5734, XP055228035, DOI: doi:10.1021/ac200998a
Attorney, Agent or Firm:
Kyoto International Patent Law Office (JP)
Patent business corporation Kyoto international patent firm (JP)
Patent business corporation Kyoto international patent firm (JP)
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