Title:
MALTOTRIOSE AMYLASE MUTANT FOR PRODUCING MALTOTRIOSE AT HIGH SPECIFICITY
Document Type and Number:
WIPO Patent Application WO/2021/243821
Kind Code:
A1
Abstract:
A maltotriose amylase mutant for producing maltotriose at high specificity, relating to the field of genetic engineering and enzyme engineering. TfAmyA from Thermobifida fusca NTU22 is modified, and is truncated, and on this basis, site-directed mutagenesis is performed on leucine at position 47, glycine at position 101, glycine at position 103, and glycine at position 153. The main product maltotriose of the obtained mutant enzyme has the specificity increased and the yield increased, which is more suitable for the production of maltotriose.
Inventors:
WU JING (CN)
WANG LEI (CN)
HU FAN (CN)
CHEN SHENG (CN)
SU LINGQIA (CN)
WANG LEI (CN)
HU FAN (CN)
CHEN SHENG (CN)
SU LINGQIA (CN)
Application Number:
PCT/CN2020/103021
Publication Date:
December 09, 2021
Filing Date:
July 20, 2020
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N9/26; C12N15/70; C12P19/00; C12P19/14
Domestic Patent References:
| WO2014068109A2 | 2014-05-08 |
Foreign References:
| CN110730824A | 2020-01-24 | |||
| CN101918545A | 2010-12-15 | |||
| CN103695387A | 2014-04-02 |
Other References:
DATABASE PROTEIN 27 July 2021 (2021-07-27), ANONYMOUS: "alpha amylase C-terminal domain-containing protein[Thermobifida fusca].", XP055877063, retrieved from GENBANK Database accession no. WP_016188446
PAN, S.H. ET AL.: "Maltooligosaccharide-forming amylase: Characteristics, preparation, and application.", BIOTECHNOLOGY ADVANCES., vol. 35, 27 April 2017 (2017-04-27), XP085118069, DOI: 10.1016/j.biotechadv.2017.04.004
YU-CHUN HUANG; YU-FEN CHEN; CHENG-YU CHEN; WEI-LIN CHEN; YONG-PING CIOU; WEN-HSIUNG LIU; CHAO-HSUN YANG;: "Production of ferulic acid from lignocellulolytic agricultural biomass bythermostable esterase produced intransformant", BIORESOURCE TECHNOLOGY, vol. 102, no. 17, 22 May 2011 (2011-05-22), AMSTERDAM, NL , pages 8117 - 8122, XP028247795, ISSN: 0960-8524, DOI: 10.1016/j.biortech.2011.05.062
DEY, T.B. ET AL.: "Improvement of microbial α-amylase stability: Strategic approaches.", PROCESS BIOCHEMISTRY., vol. 51, 18 June 2016 (2016-06-18), XP029719667, DOI: 10.1016/j.procbio.2016.06.021
CHAO-HSUN YANG ; YU-CHUN HUANG ; CHENG-YU CHEN ; CHIA-YING WEN: "Expression of Thermobifida fusca thermostable raw starch digesting alpha-amylase in Pichia pastoris and its application in raw sago starch hydrolysis", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, vol. 37, no. 4, 29 December 2009 (2009-12-29), Berlin, DE , pages 401 - 406, XP019785241, ISSN: 1476-5535
CHAO-HSUN YANG ; WEN-HSIUNG LIU: "Cloning and characterization of a maltotriose-producing α-amylase gene from Thermobifida fusca", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, vol. 34, no. 4, 9 January 2007 (2007-01-09), Berlin, DE , pages 325 - 330, XP019476529, ISSN: 1476-5535, DOI: 10.1007/s10295-006-0200-6
SUN, LIANHAI: "Study on the Relation of the Function and α-Amylase Structure", BASIC SCIENCES, CHINA MASTER’S THESES FULL-TEXT DATABASE, 15 April 2007 (2007-04-15), pages 1 - 43, XP055877127
KE, TAO: "The Directed Molecular Evolution of α-Amylase and the Analysis of the Mutants", CHINESE DOCTORAL DISSERTATIONS FULL-TEXT DATABASE (BASIC SCIENCES), 15 March 2008 (2008-03-15), pages 1 - 112, XP055877148
PAN, S.H. ET AL.: "Maltooligosaccharide-forming amylase: Characteristics, preparation, and application.", BIOTECHNOLOGY ADVANCES., vol. 35, 27 April 2017 (2017-04-27), XP085118069, DOI: 10.1016/j.biotechadv.2017.04.004
YU-CHUN HUANG; YU-FEN CHEN; CHENG-YU CHEN; WEI-LIN CHEN; YONG-PING CIOU; WEN-HSIUNG LIU; CHAO-HSUN YANG;: "Production of ferulic acid from lignocellulolytic agricultural biomass bythermostable esterase produced intransformant", BIORESOURCE TECHNOLOGY, vol. 102, no. 17, 22 May 2011 (2011-05-22), AMSTERDAM, NL , pages 8117 - 8122, XP028247795, ISSN: 0960-8524, DOI: 10.1016/j.biortech.2011.05.062
DEY, T.B. ET AL.: "Improvement of microbial α-amylase stability: Strategic approaches.", PROCESS BIOCHEMISTRY., vol. 51, 18 June 2016 (2016-06-18), XP029719667, DOI: 10.1016/j.procbio.2016.06.021
CHAO-HSUN YANG ; YU-CHUN HUANG ; CHENG-YU CHEN ; CHIA-YING WEN: "Expression of Thermobifida fusca thermostable raw starch digesting alpha-amylase in Pichia pastoris and its application in raw sago starch hydrolysis", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, vol. 37, no. 4, 29 December 2009 (2009-12-29), Berlin, DE , pages 401 - 406, XP019785241, ISSN: 1476-5535
CHAO-HSUN YANG ; WEN-HSIUNG LIU: "Cloning and characterization of a maltotriose-producing α-amylase gene from Thermobifida fusca", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, vol. 34, no. 4, 9 January 2007 (2007-01-09), Berlin, DE , pages 325 - 330, XP019476529, ISSN: 1476-5535, DOI: 10.1007/s10295-006-0200-6
SUN, LIANHAI: "Study on the Relation of the Function and α-Amylase Structure", BASIC SCIENCES, CHINA MASTER’S THESES FULL-TEXT DATABASE, 15 April 2007 (2007-04-15), pages 1 - 43, XP055877127
KE, TAO: "The Directed Molecular Evolution of α-Amylase and the Analysis of the Mutants", CHINESE DOCTORAL DISSERTATIONS FULL-TEXT DATABASE (BASIC SCIENCES), 15 March 2008 (2008-03-15), pages 1 - 112, XP055877148
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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