Title:
Β-MANNANASE MRMMAN5A AND ENCODING GENE AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2017/197546
Kind Code:
A1
Abstract:
Provided are a β-mannanase mRmMan5A and an encoding gene and an application thereof The present β-mannanase mRmMan5A has the following advantages compared to original β-mannanase mRmMan5A: optimal pH 4.5 and optimal temperature 65° C. Introducing the present protein encoding gene into pichia pastoris achieves highly effective expression. The present β-mannanase mRmMan5A is used in palm meal having undergone hydrolytic steam explosion processing, the hydrolysate mainly comprising a mannan oligosaccharide having a polymerisation degree of 2-4, the mannan oligosaccharide yield being 19.6%, and the mannan conversion rate being about 60%.
Inventors:
JIANG ZHENGQIANG (CN)
LI YANXIAO (CN)
YAN QIAOJUAN (CN)
YI PING (CN)
LIU XUEQIANG (CN)
LI YANXIAO (CN)
YAN QIAOJUAN (CN)
YI PING (CN)
LIU XUEQIANG (CN)
Application Number:
PCT/CN2016/000546
Publication Date:
November 23, 2017
Filing Date:
September 28, 2016
Export Citation:
Assignee:
UNIV CHINA AGRICULTURAL (CN)
International Classes:
C12N9/42; C12N15/11; C12N15/56; C12P19/14
Foreign References:
| CN105821020A | 2016-08-03 | |||
| CN103255119A | 2013-08-21 | |||
| CN104726481A | 2015-06-24 |
Other References:
PRITI, KATROLIA ET AL.: "Gene Cloning and Enzymatic Characterization of an Alkali- Tolerant Endo-1,4- beta -mannanase from Rhizomucor miehei", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 61, no. 2, 20 December 2012 (2012-12-20), pages 394 - 401, XP055600168, ISSN: 1520-5118, DOI: 10.1021/jf303319h
Attorney, Agent or Firm:
BEIJING WEIPINGZHIYE PATENT AGENT FIRM (CN)
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