Title:
MASS SPECTROMETRY-BASED METHOD FOR DETECTING MEDIUM- AND LOW-ABUNDANCE PROTEINS IN BODILY FLUID SAMPLE
Document Type and Number:
WIPO Patent Application WO/2023/185840
Kind Code:
A1
Abstract:
A mass spectrometry-based method for detecting medium- and low-abundance proteins of a sample, comprising: 1) removing only high-abundance proteins from a group of samples, realizing enrichment of low-abundance proteins, to be enhanced channel samples for subsequent detection; and 2) performing mass spectrometry analysis on the enhanced channel samples and on common channel samples using a high-throughput labeling method, to obtain quantitative results of each protein in bodily fluid. On the basis of the high-throughput labeling method, using an enhanced channel greatly increases mas spectrometry coverage of medium- and low-abundance proteins in blood, while retaining the high-abundance proteins in the samples.
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Inventors:
ZHU YIYING (CN)
Application Number:
PCT/CN2023/084385
Publication Date:
October 05, 2023
Filing Date:
March 28, 2023
Export Citation:
Assignee:
UNIV TSINGHUA (CN)
International Classes:
G01N1/34; G01N27/62; G01N30/02; G01N30/14; G01N30/72
Foreign References:
| CN114593979A | 2022-06-07 | |||
| CN108627647A | 2018-10-09 | |||
| CN111537658A | 2020-08-14 | |||
| CN101575370A | 2009-11-11 | |||
| CN107655998A | 2018-02-02 | |||
| CN108088933A | 2018-05-29 | |||
| CN109030834A | 2018-12-18 | |||
| US20210080469A1 | 2021-03-18 | |||
| EP2249154A1 | 2010-11-10 | |||
| US20090035849A1 | 2009-02-05 |
Attorney, Agent or Firm:
JEEKAI & PARTNERS (CN)
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