Title:
METHOD FOR ADJUSTING LENGTH OF SINGLE STRAND AT END OF DOUBLE-STRANDED DNA
Document Type and Number:
WIPO Patent Application WO/2014/123293
Kind Code:
A1
Abstract:
The present application discloses a method and kit for adjusting the length of an overhang formed at a DNA end by using competitive inhibition, and a dsDNA linkage method using same. The ligase-independent DNA linkage method using the method and kit according to the present application has improved efficiency due to the ease and accuracy of overhang length adjustment and thus can be advantageously used in various gene recombination experiments and cloning, including the cloning of a PCR product.
Inventors:
SHIN YONG KEOL (KR)
SUH YUNIL (KR)
LEE MIN YOUNG (KR)
LIM JAE YOUNG (KR)
JEONG AH RONG (KR)
SUH YUNIL (KR)
LEE MIN YOUNG (KR)
LIM JAE YOUNG (KR)
JEONG AH RONG (KR)
Application Number:
PCT/KR2013/009611
Publication Date:
August 14, 2014
Filing Date:
October 28, 2013
Export Citation:
Assignee:
ENZYNOMICS CO LTD (KR)
International Classes:
C12N15/11; C07H21/04
Domestic Patent References:
| WO1998038297A1 | 1998-09-03 |
Foreign References:
| KR930005660B1 | 1993-06-24 | |||
| KR100475305B1 | 2005-03-10 | |||
| US20120283144A1 | 2012-11-08 | |||
| US7575860B2 | 2009-08-18 |
Other References:
NISHIOKA, M. ET AL.: "Long and accurate PCR with a mixture of KOD DNA polymetase and its exonuclease deficient mutant enzyme.", JOURNAL OF BIOTECHNOLOGY., vol. 88, no. 2, 15 June 2001 (2001-06-15), pages 141 - 9, XP027295892
NISHIOKA M ET AL.: "Long and accurate PCR with a mixture of KOD DNA polymerase and its exonuclease deficient mutant enzyme", J BIOTECHNOL., vol. 88, no. 2, 15 June 2001 (2001-06-15), pages 141 - 9
NISHIOKA M ET AL.: "Long and accurate PCR with a mixture of KOD DNA polymerase and its exonuclease deficient mutant enzyme", J BIOTECHNOL., vol. 88, no. 2, 15 June 2001 (2001-06-15), pages 141 - 9
Attorney, Agent or Firm:
PARK, Weonmee (KR)
박원미 (KR)
박원미 (KR)
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