Title:
METHOD FOR AMPLIFYING IN-VITRO INDUCED CD4 +FOXP3 +CD69 +TREG AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/022599
Kind Code:
A1
Abstract:
An in-vitro induced CD4+Foxp3+CD69+Treg cell and a preparation method therefor, and a related pharmaceutical composition and use. Naive CD4+T cells are pretreated by using 10 μM of proteasome inhibitor MG132 (Z-Leu-Leu-Leu-CHO), so as to obtain at least 65% of CD4 +Foxp3 +CD69 +Treg. Compared with a control group, same is higher by 91.4 percentage points. Induced MG132 Treg cells after pretreatment have a stronger immunosuppressive activity, and induce the reestablishment of immune tolerance in the body, thereby preventing and/or treating autoimmune diseases, in particular inhibiting the development and progression of inflammatory bowel disease.
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Inventors:
JIN HONGCHUAN (CN)
YU LEI (CN)
WANG XIAN (CN)
FENG LIFENG (CN)
YU LEI (CN)
WANG XIAN (CN)
FENG LIFENG (CN)
Application Number:
PCT/CN2021/109085
Publication Date:
February 03, 2022
Filing Date:
July 28, 2021
Export Citation:
Assignee:
UNIV ZHEJIANG (CN)
International Classes:
C12N5/0783
Domestic Patent References:
| WO2020069000A1 | 2020-04-02 |
Foreign References:
| CN105338979A | 2016-02-17 | |||
| CN107083360A | 2017-08-22 |
Other References:
YU LEI, YANG FEI, ZHANG FANGHUI,GUO DANFENG, LI LING, WANG XIAN, LIANG TINGBO, WANG JIANLI, CAI ZHIJIAN, KIM HONG CHUAN: "Effects and Mechanism of CD69 Expression on the Immunoregulatory Function of Regulatory T Cells", PROCEEDINGS OF THE SIXTH GENERAL ASSEMBLY AND THE ELEVENTH CONFERENCE OF ZHEJIANG SOCIETY OF IMMUNOLOGY, 16 November 2018 (2018-11-16), pages 078 - 078, XP055890536
GAO, ZHIMEI: "Synergy Between IL-6 and TGF-β Signaling Promotes FOXP3 Degradation", MASTER THESIS, no. 3, 20 December 2011 (2011-12-20), China, pages 1 - 47, XP009533740
YU LEI, YANG FEI, ZHANG FANGHUI, GUO DANFENG, LI LING, WANG XIAN, LIANG TINGBO, WANG JIANLI, CAI ZHIJIAN, JIN HONGCHUAN: "CD69 enhances immunosuppressive function of regulatory T-cells and attenuates colitis by prompting IL-10 production", CELL DEATH & DISEASE, vol. 9, no. 905, 1 September 2018 (2018-09-01), pages 1 - 14, XP055842340, DOI: 10.1038/s41419-018-0927-9
YAN FENG, HUANG ZHENG-PING, CAI LI, XUE CHUN-YAN, DING LI-LI: "Isolation and Identification of CD4+CD25+ Regulatory T Cells", YIXUE YANJIUSHENG XUEBAO [JOURNAL OF MEDICAL POSTGRADUATES], vol. 22, no. 3, 20 March 2009 (2009-03-20), pages 231 - 235, XP055890546, ISSN: 1008-8199, DOI: 10.16571/j.cnki.1008-8199.2009.03.007
GAO, ZHIMEI: "Synergy Between IL-6 and TGF-β Signaling Promotes FOXP3 Degradation", MASTER THESIS, no. 3, 20 December 2011 (2011-12-20), China, pages 1 - 47, XP009533740
YU LEI, YANG FEI, ZHANG FANGHUI, GUO DANFENG, LI LING, WANG XIAN, LIANG TINGBO, WANG JIANLI, CAI ZHIJIAN, JIN HONGCHUAN: "CD69 enhances immunosuppressive function of regulatory T-cells and attenuates colitis by prompting IL-10 production", CELL DEATH & DISEASE, vol. 9, no. 905, 1 September 2018 (2018-09-01), pages 1 - 14, XP055842340, DOI: 10.1038/s41419-018-0927-9
YAN FENG, HUANG ZHENG-PING, CAI LI, XUE CHUN-YAN, DING LI-LI: "Isolation and Identification of CD4+CD25+ Regulatory T Cells", YIXUE YANJIUSHENG XUEBAO [JOURNAL OF MEDICAL POSTGRADUATES], vol. 22, no. 3, 20 March 2009 (2009-03-20), pages 231 - 235, XP055890546, ISSN: 1008-8199, DOI: 10.16571/j.cnki.1008-8199.2009.03.007
Attorney, Agent or Firm:
HANGZHOU JOYGO IP AGENCY FIRM (CN)
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