Title:
METHOD FOR ANALYZING MODIFIED NUCLEOSIDE
Document Type and Number:
WIPO Patent Application WO/2022/264645
Kind Code:
A1
Abstract:
A method for analyzing a modified nucleoside according to the present invention comprises: an elution step for introducing a sample containing a target component that is a modified nucleoside of which the hydrophobicity is increased by modification and a reference component that is a different component from the target component into a liquid chromatography column, and then separating the target component and the reference component from each other and eluting the target component and the reference component from the column by gradient elution such that the mixing ratio of a plurality of solvents constituting a mobile phase is changed over time; a step for detecting the target component and the reference component separately by mass spectrometry; and a step for calculating the ratio of a detected value for the target component to a detected value for the reference component. In the elution step, the mixing ratio of the solvents constituting the mobile phase to be introduced into the column is changed in such a manner that a third period in which the change ratio of the mixing ratio of the solvents constituting the mobile phase as measured at an outlet port of the column is larger than that in a first period in which the target component is eluted from the column and that in a second period in which the reference component is eluted from the column is provided between the first period and the second period.
Inventors:
KUNISAWA AKIHIRO (JP)
HATTORI TAKANARI (JP)
WATANABE JUN (JP)
TOMIZAWA KAZUHITO (JP)
NAGAYOSHI YU (JP)
SASANO RYOICHI (JP)
ASAI TOMOKI (JP)
HATTORI TAKANARI (JP)
WATANABE JUN (JP)
TOMIZAWA KAZUHITO (JP)
NAGAYOSHI YU (JP)
SASANO RYOICHI (JP)
ASAI TOMOKI (JP)
Application Number:
PCT/JP2022/016338
Publication Date:
December 22, 2022
Filing Date:
March 30, 2022
Export Citation:
Assignee:
SHIMADZU CORP (JP)
UNIV KUMAMOTO NAT UNIV CORP (JP)
AISTI SCIENCE CO LTD (JP)
UNIV KUMAMOTO NAT UNIV CORP (JP)
AISTI SCIENCE CO LTD (JP)
International Classes:
C12N15/10; B01J20/287; C12Q1/58; C12Q1/62; C12Q1/6806; C12Q1/6872; G01N27/62; G01N30/26; G01N30/34; G01N30/50; G01N30/72; G01N30/86; G01N30/88; G01N33/569
Domestic Patent References:
| WO2018124235A1 | 2018-07-05 |
Foreign References:
| JP2003014718A | 2003-01-15 | |||
| JPS6459062A | 1989-03-06 |
Attorney, Agent or Firm:
KYOTO INTERNATIONAL PATENT LAW OFFICE (JP)
Download PDF: