Title:
METHOD FOR BIOSYNTHESIZING NOOTKATONE AND CARRIER
Document Type and Number:
WIPO Patent Application WO/2023/241224
Kind Code:
A1
Abstract:
Disclosed in the present application are a method for biosynthesizing nootkatone and a carrier. The method comprises using recombinant bacteria, which is capable of expressing valentene synthase, cytochrome P450 oxidase, cytochrome P450 oxidoreductase and alcohol dehydrogenase, to synthesize nootkatone; the valentene synthase, cytochrome P450 oxidase, cytochrome P450 oxidoreductase and alcohol dehydrogenase are derived from Alpinia oxyphylla Miq. The present application identifies a complete set of enzymes and encoding genes for synthesizing nootkatone from farnesyl pyrophosphate, and provides a method for biosynthesizing nootkatone by using said enzymes. Employing the enzymes and biosynthesis method of the present application is beneficial to obtaining a high-yield strain of nootkatone and increasing the yield of biosynthetic nootkatone.
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Inventors:
YE ZILING (CN)
Application Number:
PCT/CN2023/090352
Publication Date:
December 21, 2023
Filing Date:
April 24, 2023
Export Citation:
Assignee:
WUHAN HESHENG TECH CO LTD (CN)
International Classes:
C12P7/26; C12N1/19; C12N15/52; C12N15/53; C12N15/54; C12N15/60; C12N15/61; C12N15/81; C12P5/00; C12P7/02
Foreign References:
| CN115976118A | 2023-04-18 | |||
| CN103289923A | 2013-09-11 | |||
| CN114107078A | 2022-03-01 | |||
| CN114480512A | 2022-05-13 |
Other References:
OUYANG XIAODAN, LI WEN, CHA YAPING, ZHU CHAOYI, LI SHUANG: "Production of （+）-valencene and related sesquiterpenoids by engineered Saccharomyces cerevisiae", FOOD AND FERMENTATION INDUSTRIES., vol. 45, no. 20, 31 December 2019 (2019-12-31), pages 7 - 15, XP093117894, DOI: 10.13995/j.cnki.11-1802/ts.021558
LI XIAO, REN JING-NAN, FAN GANG, ZHANG LU-LU, PAN SI-YI: "Advances on (+)-nootkatone microbial biosynthesis and its related enzymes", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, BASINGSTOKE, GB, vol. 48, no. 7-8, 24 August 2021 (2021-08-24), GB , XP093115826, ISSN: 1367-5435, DOI: 10.1093/jimb/kuab046
LI XIAO, REN JING-NAN, FAN GANG, ZHANG LU-LU, PAN SI-YI: "Advances on (+)-nootkatone microbial biosynthesis and its related enzymes", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, BASINGSTOKE, GB, vol. 48, no. 7-8, 24 August 2021 (2021-08-24), GB , XP093115826, ISSN: 1367-5435, DOI: 10.1093/jimb/kuab046
Attorney, Agent or Firm:
MERITS IP LTD. (CN)
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