Title:
METHOD FOR CAPTURING RNA IN SITU ADVANCED STRUCTURE AND INTERACTION
Document Type and Number:
WIPO Patent Application WO/2020/224040
Kind Code:
A1
Abstract:
Provided is a method for capturing a RNA in situ advanced structure and interaction. The method comprises: fixing a protein-mediated RNA interaction in a cell or tissue; perforating a membrane while maintaining the integrity of the cell; degrading a free RNA; performing pCp-biotin labeling at the tail of RNA3' and connecting proximally in situ; performing cell lysis, and then purifying the chimeric RNA containing C-biotin; constructing a strand-specific library; and performing high-throughput sequencing.
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Inventors:
XUE YUANCHAO (CN)
CAI ZHAOKUI (CN)
CAO CHANGCHANG (CN)
CAI ZHAOKUI (CN)
CAO CHANGCHANG (CN)
Application Number:
PCT/CN2019/094790
Publication Date:
November 12, 2020
Filing Date:
July 05, 2019
Export Citation:
Assignee:
INST BIOPHYSICS CAS (CN)
International Classes:
C12Q1/68
Domestic Patent References:
| WO2019071262A1 | 2019-04-11 |
Foreign References:
| CN109505012A | 2019-03-22 | |||
| CN109505012A | 2019-03-22 | |||
| CN108300767A | 2018-07-20 |
Other References:
BODO, M. ET AL.: "Simple Methods for the 3' biotinylation of RNA", RNA, vol. 20, 31 March 2014 (2014-03-31), pages 421 - 427, XP055354650, DOI: 20200207115244X
BODO, M. ET AL.: "Simple Methods for the 3' biotinylation of RNA", RNA, vol. 20, 31 March 2014 (2014-03-31), pages 421 - 427, XP055354650, DOI: 20200207125259A
BODO, M. ET AL.: "Simple Methods for the 3' biotinylation of RNA", RNA, vol. 20, 31 March 2014 (2014-03-31), pages 421 - 427, XP055354650, DOI: 20200207125259A
Attorney, Agent or Firm:
JEEKAI & PARTNERS (CN)
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