METHOD OF CONSTRUCTING DNA LIBRARY FOR SINGLE CELL GENOME SEQUENCING

WIPO Patent Application WO/2021/120959

A1

Provided is a method of constructing a DNA library for single cell genome sequencing, comprising, fragmenting DNAs in cell nuclei to obtain DNA fragmented cell nuclei; carrying out multiple rounds of marking on the fragmented DNAs in a plurality of cell nuclei by means of different sequence tags, so that the fragmented DNAs in each cell nucleus are connected with tag codes consisting of a plurality of sequence tags, and the tag codes connected with the fragmented DNAs of each cell nucleus are different; and amplifying the fragmented DNAs connected with the tag codes to obtain the DNA library for single cell genome sequencing.

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ZHAI JIXIAN (CN)
LONG YANPING (CN)
XIAO LIDAN (CN)
ZHANG FEI (CN)
LU DONGDONG (CN)
LIU ZHIJIAN (CN)

PCT/CN2020/129463

June 24, 2021

November 17, 2020

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UNIV SOUTHERN SCI & TECH (CN)

C40B50/06

CN110952147A	2020-04-03
CN110268059A	2019-09-20
CN110438572A	2019-11-12

VIJAY RAMANI, XINXIAN DENG, RUOLAN QIU, KEVIN L GUNDERSON, FRANK J STEEMERS, CHRISTINE M DISTECHE, WILLIAM S NOBLE, ZHIJUN DUAN, J: "Massively multiplex single-cell Hi-C", NAT METHODS, vol. 14, no. 3, 30 January 2017 (2017-01-30), pages 263 - 266, XP055673378, ISSN: 1548-7091, DOI: 10.1038/nmeth.4155

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