Title:
METHOD FOR CONSTRUCTING PACBIO SEQUENCING LIBRARY
Document Type and Number:
WIPO Patent Application WO/2020/259455
Kind Code:
A1
Abstract:
Provided in the present invention is a method for constructing a PacBio sequencing library, comprising the following steps: (1) obtaining a target double-stranded DNA; (2) adding a thermostable RNA ligase to respectively connect two ends of the double-stranded DNA to form a closed loop to obtain a dumbbell-shaped DNA library; (3) purifying the dumbbell-shaped DNA library; and (4) combining sequencing primers and adding a DNA polymerase to obtain a PacBio sequencing library.
Inventors:
ZHANG JIANGUANG (CN)
ZHANG HAIMAN (CN)
ZHANG XIAOJIE (CN)
SHI BO (CN)
MAO AIPING (CN)
ZHANG HAIMAN (CN)
ZHANG XIAOJIE (CN)
SHI BO (CN)
MAO AIPING (CN)
Application Number:
PCT/CN2020/097545
Publication Date:
December 30, 2020
Filing Date:
June 22, 2020
Export Citation:
Assignee:
BERRY GENOMICS CO LTD (CN)
International Classes:
C40B50/06; C12Q1/25; C12Q1/6876
Foreign References:
| CN107574245A | 2018-01-12 | |||
| CN107574245A | 2018-01-12 | |||
| CN102317475A | 2012-01-11 | |||
| CN108866172A | 2018-11-23 | |||
| CN109136222A | 2019-01-04 |
Attorney, Agent or Firm:
KING & WOOD MALLESONS (CN)
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