Title:
METHOD FOR CONSTRUCTING SENESCENT CELL AND METHOD FOR EVALUATING ANTI-SENESCENCE EFFICACY
Document Type and Number:
WIPO Patent Application WO/2023/221853
Kind Code:
A1
Abstract:
Disclosed in the present invention are a method for constructing a senescent cell and a method for evaluating the anti-senescence efficacy. The method for constructing the senescent cell comprises: performing seeding culture and subculturing culture on primary cells and performing ultraviolet light irradiation during the seeding culture and subculturing culture to obtain UVA-P1 to UVA-Pn generation cells, and according to the COL I content, MMP-1 content, and cell counting results of various generations of cells, dividing various generations of cells into early senescent cells, mid-senescent cells and late senescent cells, which can be used to evaluate the anti-senescence efficacy of skin. By means of correlating the constructed senescent cells of different stages with the senescent stage of skin in different age populations, a corresponding relationship between the generation of the senescent cells and the age of the population is established, such that the constructed senescent cells at different stages can be used as in vitro replacement cells for clinical senescence for corresponding anti-senescence evaluation.
Inventors:
ZHOU APPLE (CN)
LU INNA (CN)
GAO SERA (CN)
XIONG YASMINE (CN)
SHI MAY (CN)
LU INNA (CN)
GAO SERA (CN)
XIONG YASMINE (CN)
SHI MAY (CN)
Application Number:
PCT/CN2023/093502
Publication Date:
November 23, 2023
Filing Date:
May 11, 2023
Export Citation:
Assignee:
JAKA BIOTECH CO LTD (CN)
International Classes:
C12N13/00; C12N5/071; C12N5/077; C12Q1/02
Foreign References:
| CN114807115A | 2022-07-29 | |||
| KR20160069712A | 2016-06-17 | |||
| US20190041383A1 | 2019-02-07 | |||
| AU2020104108A4 | 2021-03-04 | |||
| CN103421763A | 2013-12-04 | |||
| CN110632303A | 2019-12-31 | |||
| EP2743342A1 | 2014-06-18 |
Other References:
ZHANG, JIE; GAO, BO: "Study on the Change of SMP30 in HSF with Induced by UVB", MILITARY MEDICAL JOURNAL OF SOUTHEAST CHINA, vol. 20, no. 2, 20 March 2018 (2018-03-20), pages 126 - 129, XP009550542, ISSN: 1672-271X
KLAUS SUSANNE; OST MARIO: "Mitochondrial uncoupling and longevity – A role for mitokines?", EXPERIMENTAL GERONTOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 130, 28 November 2019 (2019-11-28), AMSTERDAM, NL , XP085983438, ISSN: 0531-5565, DOI: 10.1016/j.exger.2019.110796
KLAUS SUSANNE; OST MARIO: "Mitochondrial uncoupling and longevity – A role for mitokines?", EXPERIMENTAL GERONTOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 130, 28 November 2019 (2019-11-28), AMSTERDAM, NL , XP085983438, ISSN: 0531-5565, DOI: 10.1016/j.exger.2019.110796
Attorney, Agent or Firm:
SHANGHAI BILIPAT INTELLECTUAL PROPERTY AGENCY LTD. (CN)
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