Title:
METHOD OF CONTROLLING ETHANOL PRODUCTION AND MASS PRODUCTION OF LACTIC ACID AND TRANSFORMANT THEREFOR
Document Type and Number:
WIPO Patent Application WO/2003/076630
Kind Code:
A1
Abstract:
It is intended to provide a transformant carrying a DNA, which encodes a foreign protein having a lactate dehydrogenase activity and exhibiting such an affinity for a pyruvic acid substrate as being comparable or even exceeding the affinity for a pyruvic acid substrate of the intrinsic pyruvate decarboxylase in the host, transferred thereinto. Using this transformant, lactic acid can be stably produced in a large amount in a host having a pyruvate decarboxylase gene.
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Inventors:
SAITO SATOSHI (JP)
SAOTOME OSAMU (JP)
YASUTANI NORIKO (JP)
MATSUO YASUO (JP)
ISHIDA NOBUHIRO (JP)
HIRAI MASANA (JP)
IMAEDA TAKAO (JP)
MIYAZAKI CHIKARA (JP)
TOKUHIRO KENRO (JP)
SAOTOME OSAMU (JP)
YASUTANI NORIKO (JP)
MATSUO YASUO (JP)
ISHIDA NOBUHIRO (JP)
HIRAI MASANA (JP)
IMAEDA TAKAO (JP)
MIYAZAKI CHIKARA (JP)
TOKUHIRO KENRO (JP)
Application Number:
PCT/JP2003/002833
Publication Date:
September 18, 2003
Filing Date:
March 11, 2003
Export Citation:
Assignee:
TOYOTA MOTOR CO LTD (JP)
SAITO SATOSHI (JP)
SAOTOME OSAMU (JP)
YASUTANI NORIKO (JP)
MATSUO YASUO (JP)
ISHIDA NOBUHIRO (JP)
HIRAI MASANA (JP)
IMAEDA TAKAO (JP)
MIYAZAKI CHIKARA (JP)
TOKUHIRO KENRO (JP)
SAITO SATOSHI (JP)
SAOTOME OSAMU (JP)
YASUTANI NORIKO (JP)
MATSUO YASUO (JP)
ISHIDA NOBUHIRO (JP)
HIRAI MASANA (JP)
IMAEDA TAKAO (JP)
MIYAZAKI CHIKARA (JP)
TOKUHIRO KENRO (JP)
International Classes:
C12N1/15; C12N9/04; C12N15/53; C12P7/56; (IPC1-7): C12N15/53; C12N9/14; C12P7/50; C12N1/15
Other References:
PORRO D. ET AL.: "Development of metabolocally engineered saccharomyces cerevisiae cells for the production of lacticacid", BIOTECHNOL. PROG., vol. 11, 1995, pages 294 - 298, XP002091315
KELLERMANN E. ET AL.: "Analysis of the primery structure and promoter function of a pyruvate decarboxylase gene (PDS1) from saccharomyces cerevisiae", NUCLEIC ACIDS RES., vol. 14, no. 22, 1986, pages 8963 - 8977, XP002961301
ADACHI E. ET AL.: "Modification of metabolic pathways of saccharomyces cerevisiae by the expression of lactate dehydrogenase and deletion of pyruvate decarboxylase genes for the lactic acid fermentation at low pH value", JOURNAL OF FERMENTATION AND BIOENGINEERING, vol. 86, no. 3, 1998, pages 284 - 289, XP002962200
BLOXHAM D.P.: "Modification of pig heart lactate dehydrogenase with methyl methanethiosulphonate to produce an enzyme with altered catalytic activity", BIOCHEMICAL JOURNAL, vol. 161, no. 3, 1977, pages 643 - 651, XP002969501
ISHIGURO N. ET AL.: "Primary structure of bovine lactate dehydrogenase-A isozyme and its synthesis in escherichia coli", GENE, vol. 91, 1990, pages 281 - 285, XP002969502
See also references of EP 1484407A4
KELLERMANN E. ET AL.: "Analysis of the primery structure and promoter function of a pyruvate decarboxylase gene (PDS1) from saccharomyces cerevisiae", NUCLEIC ACIDS RES., vol. 14, no. 22, 1986, pages 8963 - 8977, XP002961301
ADACHI E. ET AL.: "Modification of metabolic pathways of saccharomyces cerevisiae by the expression of lactate dehydrogenase and deletion of pyruvate decarboxylase genes for the lactic acid fermentation at low pH value", JOURNAL OF FERMENTATION AND BIOENGINEERING, vol. 86, no. 3, 1998, pages 284 - 289, XP002962200
BLOXHAM D.P.: "Modification of pig heart lactate dehydrogenase with methyl methanethiosulphonate to produce an enzyme with altered catalytic activity", BIOCHEMICAL JOURNAL, vol. 161, no. 3, 1977, pages 643 - 651, XP002969501
ISHIGURO N. ET AL.: "Primary structure of bovine lactate dehydrogenase-A isozyme and its synthesis in escherichia coli", GENE, vol. 91, 1990, pages 281 - 285, XP002969502
See also references of EP 1484407A4
Attorney, Agent or Firm:
Okada, Hidehiko (& Industry Building 10-19, Sakae 2-chome, Naka-k, Nagoya-shi Aichi, JP)
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