Title:
METHOD FOR CRYOPRESERVING VERO CELLS WITH HIGH VIABILITY
Document Type and Number:
WIPO Patent Application WO/2019/056217
Kind Code:
A1
Abstract:
Provided is a method for cryopreserving Vero cells with high viability, the method comprising the steps of: sucking up a culture medium with a pipette, adding PBS to wash the Vero cells twice or three times, adding 0.38% trypsin to a cell culture dish, digesting for 3 to 5 min at 37ºC, adding 2 to 3 ml of a fresh culture medium, and pipetting the bottom of the cell culture dish using the pipette 40 to 50 times; transferring the liquid to a cell centrifuge tube, centrifuging for 3 to 10 min under the condition of 1000-1500 g/min, removing the supernatant using the pipette, adding a cryopreservation liquid, vertically pipetting the cells 40 to 50 times using the pipette, adding the cells into a cell cryopreservation tube, tightening the cap of the cryopreservation tube, and sealing the cryopreservation tube with a sealing film; and preserving for 10 to 30 min at 2 to 7ºC, and for another 40 to 60 min at -15 to -20ºC, and then transferring same into an environment of ≤-80ºC for cryopreservation.
Inventors:
MA RONGHUA (CN)
Application Number:
PCT/CN2017/102485
Publication Date:
March 28, 2019
Filing Date:
September 20, 2017
Export Citation:
Assignee:
SUZHOU BEIKAI INSTR CO LTD (CN)
International Classes:
A01N1/02; C12N5/071
Foreign References:
| CN107164311A | 2017-09-15 | |||
| CN107151650A | 2017-09-12 | |||
| CN107151649A | 2017-09-12 |
Attorney, Agent or Firm:
SUZHOU FANGLVE PATENT AGENT FIRM (ORDINARY PARTNERSHIP) (CN)
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