Title:
METHOD FOR CULTIVATION OF HAIR FOLLICULAR DERMAL SHEATH CELL
Document Type and Number:
WIPO Patent Application WO/2007/037486
Kind Code:
A1
Abstract:
Disclosed is a method for cultivation of a hair follicular dermal sheath cell
or a precursor cell thereof which is a potential cellular material for hair regeneration
by cell transplantation. The method comprises the step of cultivating a hair
follicular dermal sheath cell or a precursor cell thereof in an animal cell culture
medium supplemented with a platelet-derived growth factor AA (PDGF-AA) and
a fibroblast growth factor 2 (FGF2). As a result, the hair follicular dermal sheath
cell can be proliferated while maintaining its functions, or the precursor cell
can be differentiated into a hair follicular dermal sheath cell and proliferated.
Inventors:
TOYOSHIMA KOEI (JP)
MATSUNAGA MIKARU (JP)
YOSHIZATO KATSUTOSHI (JP)
MATSUNAGA MIKARU (JP)
YOSHIZATO KATSUTOSHI (JP)
Application Number:
PCT/JP2006/319728
Publication Date:
April 05, 2007
Filing Date:
October 02, 2006
Export Citation:
Assignee:
PHOENIXBIO CO LTD (JP)
BIOINTEGRENCE INC (JP)
TOYOSHIMA KOEI (JP)
MATSUNAGA MIKARU (JP)
YOSHIZATO KATSUTOSHI (JP)
BIOINTEGRENCE INC (JP)
TOYOSHIMA KOEI (JP)
MATSUNAGA MIKARU (JP)
YOSHIZATO KATSUTOSHI (JP)
International Classes:
C12N5/071; A61L27/00
Domestic Patent References:
| WO2005053763A1 | 2005-06-16 | |||
| WO2005059119A2 | 2005-06-30 | |||
| WO2003104443A2 | 2003-12-18 | |||
| WO2003024406A2 | 2003-03-27 |
Other References:
RICHARSON G.D. ET AL.: "Cultured cells from adult human hair follicle dermis can be directed toward adipogenic and osteogenic differentiation", J. INVEST. DERMATOL., vol. 124, no. 5, May 2005 (2005-05-01), pages 1090 - 1091, XP003011055
JAHODA C.A.B. ET AL.: "Hair follicle dermal cells differentiate into adipogenic and osteogenic lineages", EXP. DERMATOL., vol. 12, 2003, pages 849 - 859, XP003011056
LAKO M. ET AL.: "Hair follicle dermal cells repopulate the mouse haematopoietic system", J. CELL SCI., vol. 115, 2002, pages 3967 - 3974, XP003011057
MCELWEE K.J. ET AL.: "Cultured peribulber dermal sheath cells can induce hair follicle development and contribute to the dermal sheath and dermal papilla", J. INVEST. DERMATOL., vol. 121, 2003, pages 1267 - 1275, XP003011058
GOODMAN L.V. AND LEDBETTER S.R.: "Secretion of stromelysin by cultured dermal papilla cells: Differential regulation by growth factors and functional role in mitogen-induced cell proliferation", J. CELL PHYSIOL., vol. 151, 1992, pages 41 - 49, XP003011059
SCHOLLMANN C. ET AL.: "Basic fibroblast growth factor modulates the mitogenic potency of the platelet-derived growth factor (PDGF) isoforms by specific upregulation of the PDGF alpha receptor in vascular smooth muscle cells", J. BIOL. CHEM., vol. 267, no. 25, 1992, pages 18032 - 18039, XP003011060
MATSUZAKI T. ET AL.: "The upper dermal sheath has a potential to regenerate the hair in the rat follicular epidermis", DIFFERENTIATION, vol. 60, 1996, pages 287 - 297, XP002913548
SHIH D.T.-B. ET AL.: "Isolation and characterization of neurogenic mesenchymal stem cells in human scalp tissue", STEM CELLS, vol. 23, August 2005 (2005-08-01), pages 1012 - 1020, XP003011061
TOMA J.G. ET AL.: "Isolation and characterization of multipotent skin-derived precursors from human skin", STEM CELLS, vol. 23, July 2005 (2005-07-01), pages 727 - 737, XP003011062
FERNANDES K.J.L. ET AL.: "A dermal niche for multipotent adult skin-derived precursor cells", NATURE CELL BIOL., vol. 6, no. 11, 2004, pages 1082 - 1093, XP003011063
TOMA J.G. ET AL.: "Isolation of multipotent adult stem cells from the dermis of mammalian skin", NATURE CELL BIOL., vol. 3, 2001, pages 778 - 784, XP003011064
JAHODA C.A.B. ET AL.: "Hair follicle dermal cells differentiate into adipogenic and osteogenic lineages", EXP. DERMATOL., vol. 12, 2003, pages 849 - 859, XP003011056
LAKO M. ET AL.: "Hair follicle dermal cells repopulate the mouse haematopoietic system", J. CELL SCI., vol. 115, 2002, pages 3967 - 3974, XP003011057
MCELWEE K.J. ET AL.: "Cultured peribulber dermal sheath cells can induce hair follicle development and contribute to the dermal sheath and dermal papilla", J. INVEST. DERMATOL., vol. 121, 2003, pages 1267 - 1275, XP003011058
GOODMAN L.V. AND LEDBETTER S.R.: "Secretion of stromelysin by cultured dermal papilla cells: Differential regulation by growth factors and functional role in mitogen-induced cell proliferation", J. CELL PHYSIOL., vol. 151, 1992, pages 41 - 49, XP003011059
SCHOLLMANN C. ET AL.: "Basic fibroblast growth factor modulates the mitogenic potency of the platelet-derived growth factor (PDGF) isoforms by specific upregulation of the PDGF alpha receptor in vascular smooth muscle cells", J. BIOL. CHEM., vol. 267, no. 25, 1992, pages 18032 - 18039, XP003011060
MATSUZAKI T. ET AL.: "The upper dermal sheath has a potential to regenerate the hair in the rat follicular epidermis", DIFFERENTIATION, vol. 60, 1996, pages 287 - 297, XP002913548
SHIH D.T.-B. ET AL.: "Isolation and characterization of neurogenic mesenchymal stem cells in human scalp tissue", STEM CELLS, vol. 23, August 2005 (2005-08-01), pages 1012 - 1020, XP003011061
TOMA J.G. ET AL.: "Isolation and characterization of multipotent skin-derived precursors from human skin", STEM CELLS, vol. 23, July 2005 (2005-07-01), pages 727 - 737, XP003011062
FERNANDES K.J.L. ET AL.: "A dermal niche for multipotent adult skin-derived precursor cells", NATURE CELL BIOL., vol. 6, no. 11, 2004, pages 1082 - 1093, XP003011063
TOMA J.G. ET AL.: "Isolation of multipotent adult stem cells from the dermis of mammalian skin", NATURE CELL BIOL., vol. 3, 2001, pages 778 - 784, XP003011064
Attorney, Agent or Firm:
NISHIZAWA, Toshio (11-1 Minami-Aoyama 6-chome,, Minato-k, Tokyo62, JP)
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