Login| Sign Up| Help| Contact|

Patent Searching and Data


Title:
METHOD FOR DETECTING ACTIVITY OF NUCLEIC ACID-METABOLIZING ENZYME
Document Type and Number:
WIPO Patent Application WO/2023/077720
Kind Code:
A1
Abstract:
Disclosed in the present invention is a method for detecting the activity of a nucleic acid-metabolizing enzyme, comprising the following steps: S1. artificially synthesizing a single-stranded nucleic acid fragment and a primer complementary from the 3' end, and forming a template by means of an annealing reaction; S2. reacting the template with a reaction buffer solution, a fluorescently-modified nucleotide derivative or fluorescently-modified nucleotide derivative analog, and a nucleic acid-metabolizing enzyme to be detected, and performing purification to obtain a primary purified product; S3. reacting the primary purified product with a Taq reaction buffer solution, dNTP, and Taq DNA polymerase, and performing purification to obtain a secondary purified product; S4. analyzing the secondary purified product by using a capillary electrophoresis instrument; and S5. creating a standard curve, and substituting the result in S4 into the standard curve to obtain a detection result.

More Like This:
JP2023511279Single cell sequencing
WO/2021/255088METHOD AND CONTROL DEVICE FOR EVALUATING A LUMINESCENCE SIGNAL IN AN ANALYSIS DEVICE FOR ANALYZING A SAMPLE OF BIOLOGICAL MATERIAL, AND ANALYSIS DEVICE FOR ANALYZING A SAMPLE OF BIOLOGICAL MATERIAL
JP2024038208Methods and reagents for detecting and evaluating genotoxicity
Inventors:
ZHOU RONG (CN)
WANG PAN (CN)
Application Number:
PCT/CN2022/086204
Publication Date:
May 11, 2023
Filing Date:
April 12, 2022
Export Citation:
Click for automatic bibliography generation   Help
Assignee:
ME INSTR INC (CN)
International Classes:
C12Q1/686; C12Q1/48
Domestic Patent References:
WO2008024052A12008-02-28
WO2015058104A12015-04-23
Foreign References:
CN114250281A2022-03-29
CA2233701A11998-02-19
US20120070838A12012-03-22
US20060057596A12006-03-16
Other References:
SCHRIEK S., KAHMANN U., STAIGER D., PISTORIUS E. K., MICHEL K.-P.: "Detection of an L-amino acid dehydrogenase activity in Synechocystis sp. PCC 6803", JOURNAL OF EXPERIMENTAL BOTANY, OXFORD UNIVERSITY PRESS, GB, vol. 60, no. 3, 1 March 2009 (2009-03-01), GB , pages 1035 - 1046, XP093062219, ISSN: 0022-0957, DOI: 10.1093/jxb/ern352
Attorney, Agent or Firm:
SHENZHEN KEGUAN INTELLECTUAL PROPERTY AGENCY CO., LTD (CN)
Download PDF:
View/Download PDF      PDF Help



 
Previous Patent: PRECISE FLYKNIT FABRIC AND MANUFACTURING METHOD THEREFOR

Next Patent: SPEED INCREASING MECHANISM FOR SCARA ROBOT, AND SCARA ROBOT HAVING SPEED INCREASING MECHANISM