Title:
METHOD OF DETECTING BIOLOGICAL MATERIAL, AND CHEMILUMINESCENT INDICATOR USED THEREIN
Document Type and Number:
WIPO Patent Application WO/2018/139614
Kind Code:
A1
Abstract:
Provided is a method of detecting a biological material capable of facilitating quantitative measurement. A method of detecting a biological material in a sample includes mixing with the sample both a fusion protein (C) in which a protein (A) capable of binding a biological material and a chemiluminescent protein (B) are fused, and a substrate of the chemiluminescent protein (B), and observing a luminescent signal from the sample. In this detecting method: the protein (A) and the protein (B) are linked in such a way as to allow resonance energy transfer; the protein (A) is a protein (A1) capable of emitting fluorescence when binding the biological material, or a protein (A2) capable of binding a biological material that is an autofluorescent molecule; and the protein (B) is capable of exciting fluorescence or autofluorescence of the protein (A) by means of the luminescent energy thereof.
More Like This:
Inventors:
NAGAI TAKEHARU (JP)
ARAI YOSHIYUKI (JP)
ARAI YOSHIYUKI (JP)
Application Number:
PCT/JP2018/002587
Publication Date:
August 02, 2018
Filing Date:
January 26, 2018
Export Citation:
Assignee:
UNIV OSAKA (JP)
International Classes:
G01N21/78; G01N21/64; G01N33/53; G01N33/542
Domestic Patent References:
| WO2016001437A2 | 2016-01-07 | |||
| WO2014133158A1 | 2014-09-04 | |||
| WO2014133158A1 | 2014-09-04 |
Foreign References:
| JP2016500263A | 2016-01-12 |
Other References:
KUMAGAI ET AL., CELL, vol. 153, 2013, pages 1602 - 1611
"UniProtKB/Swiss-Prot", Database accession no. PODM59.1
"GenBank", Database accession no. AB763906.1
RODRIGUEZ ET AL., NATURE METHODS, vol. 13, 2016, pages 763 - 769
SHU X ET AL., SCIENCE, vol. 324, no. 5928, 2009, pages 804 - 8,7
FILONOV GS ET AL., NAT BIOTECH, vol. 29, no. 8, 2011, pages 757 - 761
DREPPER T. ET AL., NAT BIOTECH., vol. 25, no. 4, 2007, pages 443 - 445
CHAPMAN S. ET AL., PNAS, vol. 105, no. 50, 2008, pages 20038 - 43
SHU X. ET AL., PLOS BIOL., vol. 9, no. 4, 2011
See also references of EP 3575779A4
"UniProtKB/Swiss-Prot", Database accession no. PODM59.1
"GenBank", Database accession no. AB763906.1
RODRIGUEZ ET AL., NATURE METHODS, vol. 13, 2016, pages 763 - 769
SHU X ET AL., SCIENCE, vol. 324, no. 5928, 2009, pages 804 - 8,7
FILONOV GS ET AL., NAT BIOTECH, vol. 29, no. 8, 2011, pages 757 - 761
DREPPER T. ET AL., NAT BIOTECH., vol. 25, no. 4, 2007, pages 443 - 445
CHAPMAN S. ET AL., PNAS, vol. 105, no. 50, 2008, pages 20038 - 43
SHU X. ET AL., PLOS BIOL., vol. 9, no. 4, 2011
See also references of EP 3575779A4
Attorney, Agent or Firm:
IKEUCHI SATO & PARTNER PATENT ATTORNEYS (JP)
Download PDF: