METHOD FOR DETECTING DIFFERENTIALLY METHYLATED CPG ISLANDS ASSOCIATED WITH ABNORMAL STATE OF HUMAN BODY

Title:

METHOD FOR DETECTING DIFFERENTIALLY METHYLATED CPG ISLANDS ASSOCIATED WITH ABNORMAL STATE OF HUMAN BODY

Document Type and Number:

WIPO Patent Application WO/2016/101258

Kind Code:

A1

Abstract:

Disclosed is a method for detecting differentially methylated CpG islands associated with an abnormal state of a human body, characterized by detecting very minute amounts of methylated CpG short tandem nucleic acid sequences in highly fragmented DNA samples with genome scale, identifying differentially methylated CpG islands associated with abnormal state of human body and determining the corresponding abnormal state of human body. Sequencing libraries are constructed by using CpG short tandem sequences as primers to perform three steps of PCR reactions on DNAs which are conversed by modifiers, and detections of very minute amounts of methylated CpG short tandem nucleic acid sequences are implemented with high throughput sequencing technology. A group of genome sequences and methylation patterns of differentially methylated CpG islands which are associated with hepatocellular carcinoma are also disclosed; they may be used for distinguishing between hepatocellular carcinoma and non-cancerous state.

Inventors:

WEN LU (CN)
PENG JIRUN (CN)
HUANG YANYI (CN)
TANG FUCHOU (CN)
LIU XIAOMENG (CN)
LI JINGYI (CN)
GUO HUAHU (CN)

Application Number:

PCT/CN2014/095120

Publication Date:

June 30, 2016

Filing Date:

December 26, 2014

Export Citation:

Click for automatic bibliography generation Help

Assignee:

UNIV BEIJING (CN)
BEIJING SHIJITAN HOSPITAL CAPITAL MEDICAL UNIVERSITY (CN)

International Classes:

C12Q1/68

Foreign References:

CN101160411A	2008-04-09
CN101230399A	2008-07-30

Other References: