Title:
METHOD FOR DETECTING FUSOBACTERIUM NUCLEATUM AND METHOD FOR DETECTING FUSOBACTERIUM NUCLEATUM NUCLEATUM
Document Type and Number:
WIPO Patent Application WO/2024/053642
Kind Code:
A1
Abstract:
According to one aspect of the present invention, this method for detecting Fusobacterium nucleatum in a sample involves a step for subjecting a DNA derived from a sample to nucleic acid amplification reaction using a primer set specific to a citrate lyase beta subunit gene of Fusobacterium nucleatum. According to said method, F. nucleatum can be specifically detected. According to another aspect of the present invention, this method for detecting Fusobacterium nucleatum nucleatum in a sample involves a step for subjecting a DNA derived from a sample to nucleic acid amplification reaction using a primer set specific to a dicarboxylate/amino acid: cation cotransporter gene of Fusobacterium nucleatum nucleatum. According to said method, F. nucleatum nucleatum can be specifically detected.
Inventors:
KAKU TOSHISUKE (JP)
Application Number:
PCT/JP2023/032374
Publication Date:
March 14, 2024
Filing Date:
September 05, 2023
Export Citation:
Assignee:
EIKEN CHEMICAL (JP)
International Classes:
C12Q1/04; C12N15/31; C12N15/60; C12Q1/6844; C12Q1/6876; C12Q1/689
Domestic Patent References:
| WO2023042714A1 | 2023-03-23 |
Other References:
DATABASE Nucleotide 3 August 2016 (2016-08-03), ANONYMOUS : "Fusobacterium nucleatum subsp. nucleatum ATCC 25586 chromosome, complete genome", XP093147941, retrieved from NCBI Database accession no. NC_003454.1
MORSI HANIE, GOLIZEH MAKAN, BROSSEAU NOAH, JANATI AMAL IDRISSI, EMAMI ELHAM, NDAO MOMAR, TRAN SIMON D.: "Detection of Fusobacterium nucleatum subspecies in the saliva of pre-colorectal cancer patients, using tandem mass spectrometry", ARCHIVES OF ORAL BIOLOGY, PERGAMON PRESS, OXFORD., GB, vol. 134, 1 February 2022 (2022-02-01), GB , pages 105337, XP093147938, ISSN: 0003-9969, DOI: 10.1016/j.archoralbio.2021.105337
KAVITHA M.; KUMAR N. VINOD; SREEDEVI B.: "Standardization of loop-mediated isothermal amplification for detection of D. nodosus and F. necrophorum causing footrot in sheep and goats", TROPICAL ANIMAL HEALTH AND PRODUCTION, SPRINGER NETHERLANDS, DORDRECHT, vol. 54, no. 1, 15 January 2022 (2022-01-15), Dordrecht, XP037665455, ISSN: 0049-4747, DOI: 10.1007/s11250-022-03064-3
MORSI HANIE, GOLIZEH MAKAN, BROSSEAU NOAH, JANATI AMAL IDRISSI, EMAMI ELHAM, NDAO MOMAR, TRAN SIMON D.: "Detection of Fusobacterium nucleatum subspecies in the saliva of pre-colorectal cancer patients, using tandem mass spectrometry", ARCHIVES OF ORAL BIOLOGY, PERGAMON PRESS, OXFORD., GB, vol. 134, 1 February 2022 (2022-02-01), GB , pages 105337, XP093147938, ISSN: 0003-9969, DOI: 10.1016/j.archoralbio.2021.105337
KAVITHA M.; KUMAR N. VINOD; SREEDEVI B.: "Standardization of loop-mediated isothermal amplification for detection of D. nodosus and F. necrophorum causing footrot in sheep and goats", TROPICAL ANIMAL HEALTH AND PRODUCTION, SPRINGER NETHERLANDS, DORDRECHT, vol. 54, no. 1, 15 January 2022 (2022-01-15), Dordrecht, XP037665455, ISSN: 0049-4747, DOI: 10.1007/s11250-022-03064-3
Attorney, Agent or Firm:
HASEGAWA Yoshiki et al. (JP)
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