Title:
METHOD FOR DETECTING NUCLEIC ACID, AND KIT FOR SAID METHOD
Document Type and Number:
WIPO Patent Application WO/2018/066713
Kind Code:
A1
Abstract:
[Problem] To provide a method for detecting a nucleic acid, whereby it becomes possible to detect a nucleic acid such as miRNA within a short time and in a nucleotide sequence-specific manner without requiring the use of an expensive apparatus.
[Solution] A method for detecting a target nucleic acid having a specific nucleotide sequence in a sample comprises: a double-stranded nucleic acid formation step of hybridizing the target nucleic acid with a liposome-bonded single-stranded nucleic acid to form a double-stranded nucleic acid, wherein the liposome-bonded single-stranded nucleic acid is composed of a labeled liposome bonded to a single-stranded nucleic acid, is immobilized onto a support or is so modified as to be capable of being immobilized onto a support, and contains a nucleotide sequence complementary to the target nucleic acid; a liposome liberation step of allowing a double-strand-specific nuclease to act on the formed double-stranded nucleic acid to cleave the nucleic acid and thereby liberate the liposome; and a label detection step of detecting a label in the liberated liposome.
Inventors:
FUJII SATOSHI (JP)
KAMIYA KOKI (JP)
OSAKI TOSHIHISA (JP)
MISAWA NORIO (JP)
TAKEUCHI SHOJI (JP)
KAMIYA KOKI (JP)
OSAKI TOSHIHISA (JP)
MISAWA NORIO (JP)
TAKEUCHI SHOJI (JP)
Application Number:
PCT/JP2017/036565
Publication Date:
April 12, 2018
Filing Date:
October 03, 2017
Export Citation:
Assignee:
KANAGAWA INSTITUTE OF INDUSTRIAL SCIENCE AND TECH (JP)
International Classes:
C12Q1/68; C12N15/09
Foreign References:
| JP2002507128A | 2002-03-05 | |||
| JPS6091999A | 1985-05-23 | |||
| JPH0292300A | 1990-04-03 | |||
| JPH02308800A | 1990-12-21 | |||
| JPH06181800A | 1994-07-05 |
Other References:
FUJII, SATOSHI ET AL.: "Development of method to detect microRNA in the presence of contaminants", CHEMINAS35, vol. 35, 22 May 2017 (2017-05-22), pages 98
FUJII, S. ET AL.: "SPIRAL CHANNEL FOR FAST AND NOISE-FREE-MICRORNA DETECTION", IEEE CONFERENCE PROCEEDINGS, January 2017 (2017-01-01), pages 406 - 407, XP033069591, DOI: doi:10.1109/MEMSYS.2017.7863427
FUJII, SATOSHI ET AL.: "Single molecule detection usign artificial cell membrane", CHEMISTRY TODAY, no. 545, August 2016 (2016-08-01), pages 53 - 57, ISSN: 0386-961X
27 July 2015 (2015-07-27), pages 39 - 41, Retrieved from the Internet [retrieved on 20171208]
FUJII, S. ET AL.: "SPIRAL CHANNEL FOR FAST AND NOISE-FREE-MICRORNA DETECTION", IEEE CONFERENCE PROCEEDINGS, January 2017 (2017-01-01), pages 406 - 407, XP033069591, DOI: doi:10.1109/MEMSYS.2017.7863427
FUJII, SATOSHI ET AL.: "Single molecule detection usign artificial cell membrane", CHEMISTRY TODAY, no. 545, August 2016 (2016-08-01), pages 53 - 57, ISSN: 0386-961X
27 July 2015 (2015-07-27), pages 39 - 41, Retrieved from the Internet
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