Title:
METHOD FOR DETECTING AND SORTING ANTIGEN-SPECIFIC T-CELL RECEPTOR WITH HIGH THROUGHPUT
Document Type and Number:
WIPO Patent Application WO/2023/019583
Kind Code:
A1
Abstract:
A method for detecting and sorting an antigen-specific T-cell receptor. According to the method, a liquid droplet only containing a TCR-expressing cell is prepared by means of using a microfluidic technique, thereby achieving high-throughput detection and sorting of different TCRs. With the help of the droplet microfluidic technique, millions of droplets can be prepared in one step, and cells can independently detect the antigen specificity in the droplets. Compared with detecting the cell interaction in a well plate, the throughput is significantly improved.
Inventors:
WANG SHIYU (CN)
LI YIJIAN (CN)
GAO KAI (CN)
LIU YA (CN)
DONG XUAN (CN)
LIU YANG (CN)
ZHANG JUANYU (CN)
CHEN LINZHE (CN)
LV MENGHUA (CN)
SUN NA (CN)
LI YIJIAN (CN)
GAO KAI (CN)
LIU YA (CN)
DONG XUAN (CN)
LIU YANG (CN)
ZHANG JUANYU (CN)
CHEN LINZHE (CN)
LV MENGHUA (CN)
SUN NA (CN)
Application Number:
PCT/CN2021/113888
Publication Date:
February 23, 2023
Filing Date:
August 20, 2021
Export Citation:
Assignee:
BGI SHENZHEN (CN)
International Classes:
G01N21/64; A61P35/00
Foreign References:
| CN112165989A | 2021-01-01 | |||
| CN107064091A | 2017-08-18 | |||
| CN110431233A | 2019-11-08 | |||
| EP3819637A1 | 2021-05-12 | |||
| US20160223532A1 | 2016-08-04 |
Other References:
HUANG HUANG; WANG CHUNLIN; RUBELT FLORIAN; SCRIBA THOMAS J.; DAVIS MARK M.: "Analyzing theimmune response by T-cell receptor clustering with GLIPH2 and genome-wide antigen screening", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP US, NEW YORK, vol. 38, no. 10, 27 April 2020 (2020-04-27), New York, pages 1194 - 1202, XP037261742, ISSN: 1087-0156, DOI: 10.1038/s41587-020-0505-4
Attorney, Agent or Firm:
IP MARCH (CN)
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