Title:
METHOD OF DETECTING TARGET NUCLEIC ACID
Document Type and Number:
WIPO Patent Application WO/2001/030993
Kind Code:
A1
Abstract:
A method of specifically detecting a target nucleic acid in a biological material characterized by comprising: (a) the step of bringing the target nucleic acid or a chain complementary thereto into contact with a first labeled nucleic acid primer complementary to either, or a primer set containing this primer to thereby form a primer extension product; (b) the step of denaturing the first nucleic acid primer extension product as described above to give a denatured chain; (c) the step of bringing this denatured chain into contact with a second labeled nucleic acid primer complementary thereto to form a both-labeled primer extension product; and (d) the step of developing this both-labeled primer extension product on a chromatographic carrier having specifically binding reagents which bind specifically to the labels of the first labeled nucleic acid primer and the second labeled nucleic acid primer; and a kit for detecting a target nucleic acid with the use of this method. Use of the detection method/detection kit makes it possible to conveniently, quickly and highly sensitively detect the presence or absence of the target nucleic acid in a sample.
Inventors:
MITANI YASUMASA (JP)
Application Number:
PCT/JP2000/007430
Publication Date:
May 03, 2001
Filing Date:
October 24, 2000
Export Citation:
Assignee:
WAKUNAGA PHARMA CO LTD (JP)
MITANI YASUMASA (JP)
MITANI YASUMASA (JP)
International Classes:
C12Q1/68; C12Q1/686; (IPC1-7): C12N15/09; C12Q1/68
Domestic Patent References:
| WO1994026934A2 | 1994-11-24 |
Foreign References:
| JPH05176800A | 1993-07-20 | |||
| EP0439222A2 | 1991-07-31 | |||
| JPH0775599A | 1995-03-20 |
Attorney, Agent or Firm:
Aruga, Mitsuyuki (3-6 Nihonbashiningyocho 1-chome Chuo-ku, Tokyo, JP)
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