NOVEL RHODOCOCCUS, RHODOCOCCUS-ORIGIN NITRILASE GENE, NITRILEHYDRATASE GENE AND AMIDASE GENE AND PROCESS FOR PRODUCING CARBOXYLIC ACIDS BY USING THE SAME

Title:

NOVEL RHODOCOCCUS, RHODOCOCCUS-ORIGIN NITRILASE GENE, NITRILEHYDRATASE GENE AND AMIDASE GENE AND PROCESS FOR PRODUCING CARBOXYLIC ACIDS BY USING THE SAME

Document Type and Number:

WIPO Patent Application WO/2001/030994

Kind Code:

A1

Abstract:

A novel rhodococcus and a process for producing carboxylic acids by hydrolyzing cyano group of nitrile compounds by using this bacterium. A process for producing carboxylic acids (in particular, cyanocarboxylic acids) by using transformants having been transformed by plasmids containing rhodococcus-origin nitrilase gene, nitrilehydratase gene and amidase gene showing an excellent site-selectivity to cyano group of aromatic polynitrile compounds; these transformants, plasmids, genes and a process for producing enzymes by using these transformants; and the enzymes thus obtained. The carboxylic acids (in particular, cyanocarboxylic acids) thus obtained are useful as starting materials in the synthesis of drugs, pesticides, dyes and other chemical products.

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Inventors:

AOKI HIROBUMI (JP)
KAMACHI HARUMI (JP)

Application Number:

PCT/JP2000/007464

Publication Date:

May 03, 2001

Filing Date:

October 25, 2000

Export Citation:

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Assignee:

SHOWA DENKO KK (JP)
AOKI HIROBUMI (JP)
KAMACHI HARUMI (JP)

International Classes:

C12N1/21; C12N9/78; C12N15/55; C12P7/40; (IPC1-7): C12N15/09; C12N15/55; C12N1/21; C12N9/78; C12P13/02; C07K14/37

Foreign References:

JPH0799980A	1995-04-18
EP0445646A2	1991-09-11
US4629700A	1986-12-16
US4629700A	1986-12-16
EP0178106A2	1986-04-16

Other References:

MICHIHIKI KOBAYASHI ET AL.: "Nitrilase from rhodococcus rhodochrous J1", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 267, no. 29, 1992, pages 20746 - 20751, XP002936322
MICHIKO KOBAYASHI ET AL.: "Amidase coupled with low-molecular-mass nitrile hydratase from rhodococcus rhodochrous J1", EUR. J. BIOCHEM., vol. 217, no. 1, 1993, pages 327 - 336, XP002936323
JONATHAN HUGHES ET AL.: "Application of whole cell rhodococcal biocatalysts in acrylic polymer manufacture", ANTONIE VAN LEEUWENHOEK, vol. 74, no. 1-3, 1998, pages 107 - 118, XP002936324

Attorney, Agent or Firm:

Shiga, Masatake (Takadanobaba 3-chome Shinjuku-ku Tokyo, JP)

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