Title:
METHOD OF DETERMINING BASE SEQUENCE OF NUCLEIC ACID
Document Type and Number:
WIPO Patent Application WO/2003/074698
Kind Code:
A1
Abstract:
A method appropriate for the high throughput system wherein shotgun sequencing is performed over a wide region of a genome sequence or the like to thereby mostly determine the base sequence and then the gap region, the sequence of which cannot be determined, can be efficiently sequenced without preparing a large amount of DNA or without restricted by modification of DNA.
Inventors:
TOMONO JUN (JP)
TAKEDA OSAMU (JP)
KITAGAWA MASANARI (JP)
ASADA KIYOZO (JP)
KATO IKUNOSHIN (JP)
TAKEDA OSAMU (JP)
KITAGAWA MASANARI (JP)
ASADA KIYOZO (JP)
KATO IKUNOSHIN (JP)
Application Number:
PCT/JP2003/002546
Publication Date:
September 12, 2003
Filing Date:
March 05, 2003
Export Citation:
Assignee:
TAKARA BIO INC (JP)
TOMONO JUN (JP)
TAKEDA OSAMU (JP)
KITAGAWA MASANARI (JP)
ASADA KIYOZO (JP)
KATO IKUNOSHIN (JP)
TOMONO JUN (JP)
TAKEDA OSAMU (JP)
KITAGAWA MASANARI (JP)
ASADA KIYOZO (JP)
KATO IKUNOSHIN (JP)
International Classes:
G01N27/447; C12N15/09; C12Q1/68; (IPC1-7): C12N15/09; C12Q1/68
Domestic Patent References:
| WO1998026096A1 | 1998-06-18 | |||
| WO2000066768A1 | 2000-11-09 | |||
| WO1998049341A2 | 1998-11-05 |
Other References:
TETTELIN H. ET AL.: "Optimized multiplex PCR: efficiently closing a whole-genome shotgun sequencing project", GENOMICS, vol. 62, no. 3, 1999, pages 500 - 507, XP004444704
RENA G. ET AL.: "A simple method for sequencing small DNAs by introducing precise overlapping ends into restriction digestion fragments", NUCLEIC ACIDS RES., vol. 26, no. 16, 1998, pages 3867 - 3868, XP002962171
RENA G. ET AL.: "A simple method for sequencing small DNAs by introducing precise overlapping ends into restriction digestion fragments", NUCLEIC ACIDS RES., vol. 26, no. 16, 1998, pages 3867 - 3868, XP002962171
Attorney, Agent or Firm:
Aoyama, Tamotsu (IMP Building C3-7 Shiromi 1-chome, Chuo-k, Osaka-shi Osaka, JP)
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