Title:
METHOD FOR DETERMINING NON-HOMOLOGOUS END JOINING REPAIR ACTIVITY
Document Type and Number:
WIPO Patent Application WO/2017/024602
Kind Code:
A1
Abstract:
Provided is a method for determining non-homologous end joining repair activity, In the method, an HPRT gene is mutated by using a gene site-directed mutagenesis technique, then, the steps of plasmid transfection, drug treatment and 6-TG treatment need to be performed, and finally, the NHEJ repair activity is observed by means of cell activity detection.
Inventors:
LIU FENJU (CN)
DU JIE (CN)
YU JIAHUA (CN)
ZHANG HAOWEN (CN)
SHANG ZENGFU (CN)
ZHANG YUSHUO (CN)
DU JIE (CN)
YU JIAHUA (CN)
ZHANG HAOWEN (CN)
SHANG ZENGFU (CN)
ZHANG YUSHUO (CN)
Application Number:
PCT/CN2015/087321
Publication Date:
February 16, 2017
Filing Date:
August 18, 2015
Export Citation:
Assignee:
ZHANGJIAGANG INST IND TECHNOLOGIES SOOCHOW UNIV (CN)
International Classes:
C12N5/10; C12N15/85; C12Q1/02; C12Q1/48; C12Q1/68
Domestic Patent References:
| WO2012021632A2 | 2012-02-16 | |||
| WO2012138927A2 | 2012-10-11 |
Foreign References:
| CN103388006A | 2013-11-13 | |||
| CN104673816A | 2015-06-03 |
Attorney, Agent or Firm:
CENTRAL SOUTH WELL INTELLECTUAL PROPERTY OFFICE (CN)
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