Title:
METHOD AND DEVICE FOR SYNTHESIZING PROTEIN FROM DNA MOLECULE CAPTURED IN MICROCHAMBER
Document Type and Number:
WIPO Patent Application WO/2011/070776
Kind Code:
A1
Abstract:
Provided are a method and a device for synthesizing a protein from a DNA molecule captured in a microchamber, whereby the distance between microchambers can be shortened and thus the density of an array can be increased.
Microchambers (32) are arranged at a high density. A DNA solution (34), which has been diluted so that one DNA molecule on average can be captured, is enclosed in the microchambers (32). Then, mRNA is synthesized using the one DNA molecule on average as a template. Based on this mRNA, a protein (37) is extracellularly synthesized.
Inventors:
FUJII TERUO (JP)
KIM SOO-HYEON (JP)
TAKEUCHI SHOJI (JP)
FOURMY DOMINIQUE (JP)
YOSHIZAWA SATOKO (JP)
KIM SOO-HYEON (JP)
TAKEUCHI SHOJI (JP)
FOURMY DOMINIQUE (JP)
YOSHIZAWA SATOKO (JP)
Application Number:
PCT/JP2010/007144
Publication Date:
June 16, 2011
Filing Date:
December 08, 2010
Export Citation:
Assignee:
CENTRE NAT RECH SCIENT (FR)
FUJII TERUO (JP)
KIM SOO-HYEON (JP)
TAKEUCHI SHOJI (JP)
FOURMY DOMINIQUE (JP)
YOSHIZAWA SATOKO (JP)
FUJII TERUO (JP)
KIM SOO-HYEON (JP)
TAKEUCHI SHOJI (JP)
FOURMY DOMINIQUE (JP)
YOSHIZAWA SATOKO (JP)
International Classes:
C12P21/00; C12M1/00; C12N15/09
Foreign References:
| JP3727026B2 | 2005-12-14 |
Other References:
YASUTAKA MORITA ET AL.: "Chip Gijutsu to Renkei shita Combinatorial Sekkeiho ni yoru Kino Bunshi no Sosei", BIOTECHNOLOGY, vol. 79, 2001, pages 367 - 370, XP008160308
SAKAI-KATO K. ET AL.: "An enzyme-immobilization method for integration of biofunctions on a microchip using a water-soluble amphiphilic phospholipid polymer having a reacting group.", LAB CHIP, vol. 4, 2004, pages 4 - 6, XP008159168
XU Y. ET AL.: "Microfluidic flow control on charged phospholipid polymer interface.", LAB CHIP, vol. 7, 2007, pages 199 - 206, XP008159120
KAZUHIKO ISHIHARA ET AL.: "Bio Device Hyomen no Nano Seimitsu Sosei", GEKKAN FUNCTION & MATERIALS, vol. 27, no. 5, 2007, pages 7 - 15, XP008159572
MADOKA TAKAI: "Bio Device Sosei no Tameno Kinosei Nano Interface", MATERIAL INTEGRATION, vol. 20, no. 9, 2007, pages 44 - 49, XP008159117
RUNGPRAGAYPHAN S. ET AL.: "High-throughput, cloning-independent protein library construction by combining single-molecule DNA amplification with in vitro expression.", J. MOL. BIOL., vol. 318, 2002, pages 395 - 405, XP008159142
RUNGPRAGAYPHAN S. ET AL.: "Rapid screening for affinity-improved scFvs by means of single- molecule-PCR-linked in vitro expression.", J. MOL. CATAL. B ENZYM., vol. 28, 2004, pages 223 - 228, XP008159207
KOGA Y. ET AL.: "In vitro construction and screening of a Burkholderia cepacia lipase library using single-molecule PCR and cell-free protein synthesis.", J. BIOSCI. BIOENG., vol. 94, 2002, pages 84 - 86, XP008159635
KOGA Y. ET AL.: "Inverting enantioselectivity of Burkholderia cepacia KWI-56 lipase by combinatorial mutation and high-throughput screening using single-molecule PCR and in vitro expression.", J. MOL. BIOL., vol. 331, 2003, pages 585 - 592, XP008159202
EIICHI TAMITANI: "DNA1 Bunshi kara no Protein Device eno Shin Tenkai", CHEMISTRY, vol. 57, no. 12, 2002, pages 37 - 38, XP008159548
NAKANO H. ET AL.: "Single-step single-molecule PCR of DNA with a homo-priming sequence using a single primer and hot-startable DNA polymerase.", J. BIOSCI. BIOENG., vol. 90, 2000, pages 456 - 458, XP008159244
See also references of EP 2511378A4
ANDREI KOROSTELEV; HARRY F. NOLLER: "The Ribosome in Focus: New Structures bring New Insights", TRENDS IN BIOCHEMICAL SCIENCES, vol. 32, no. 9, 2007, pages 434 - 441
R. ANDREW MARSHALL; COLLIN ECHEVERRIA AITKEN; MAGDALENA DORYWALSKA; JOSEPH D. PUGLISI: "Translation at the Single-Molecule Level", ANNUAL REVIEW OF BIOCHEMISTRY, vol. 77, 2008, pages 177 - 203
BORIS ROTMAN: "Measurement of Activity of Single Molecules of (3-D-Galactosidase", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 47, 1961, pages 1981 - 1991
DAN S. TAWFIK; ANDREW D. GRIFFITHS: "Man-Made Cell-Like Compartments for Molecular Evolution", NATURE BIOTECHNOLOGY, vol. 16, 1998, pages 652 - 656
YANNICK RONDELEZ; GUILLAUME TRESSET; KAZUHITO V. TABATA; HIDEYUKI ARATA; HIROYUKI FUJITA; SHOJI TAKEUCHI; HIROYUKI NOJI: "Microfabricated arrays of femtoliter chambers allow single molecule enzymology", NATURE BIOTECHNOLOGY, vol. 23, 2005, pages 361 - 365
TAKESHI KINPARA; RYUTA MIZUNO; YUJI MURAKAMI; MASAAKI KOBAYASHI; SHOHEI YAMAURA; QUAMRUL HASAN; YASUTSUKA MORITA; HIDEO NAKANO; TS: "A Picoliter Chamber Array for Cell-Free Protein Synthesis", JOURNAL OF BIOCHEMISTRY, vol. 136, no. 2, 2004, pages 149 - 154
TAKAOKI YAMAMOTO; TAKAHIKO NOJIMA; TERUO FUJII: "PDMS-Glass Hybrid Microreactor Array with embedded Temperature Control Device. Application to Cell-Free Protein Synthesis", LAB ON A CHIP, vol. 2, 2002, pages 197 - 202
MARI TABUCHI; MAMI HINO; YASUO SHINOHARA; YOSHINOBU BABA: "Cell-Free Protein Synthesis on a Microchip", PROTEOMICS, vol. 2, 2002, pages 430 - 435
PHILIPP ANGENENDT; LAJOS NYARSIK; WITOLD SZAFLARSKI; JORN GLOKLER; KNUD H. NIERHAUS; HANS LEHRACH; DOLORES J. CAHILL; ANGELIKA LUE: "Cell-Free Protein Expression and Functional Assay in Nanowell Chip Format", ANALYTICAL CHEMISTRY, vol. 76, no. 7, 2004, pages 1844 - 1849
PHILIPP ANGENENDT; JURGEN KREUTZBERGER; JORN GLOKLER; JORD D. HOHEISEL: "Generation of High Density Protein Microarrays by Cell-Free in Situ Expression of Unpurified PCR Products", MOLECULAR & CELLULAR PROTEOMICS, vol. 5, no. 9, 2006, pages 1658 - 1666
SHENG-CE TAO; HENG ZHU: "Protein Chip Fabrication by Capture of Nascent Polypeptides", NATURE BIOTECHNOLOGY, vol. 24, no. 10, 2006, pages 1253 - 1254
NIROSHAN RAMACHANDRAN; JACOB V. RAPHAEL; EUGENIE HAINSWORTH; GOKHAN DEMIRKAN; MANUEL G. FUENTES; ANDREAS ROLFS; YANHUI HU; JOSHUA: "Next-Generation High-Density Self-Assembling Functional Protein Arrays", NATURE METHODS, vol. 5, 2008, pages 535 - 538
MINGYUE HE; ODA STOEVESANDT; ELIZABETH A. PALMER; FARID KHAN; OLLE ERICSSON; MICHAEL TAUSSIG: "Printing Protein Arrays from DNA Arrays", NATURE METHODS, vol. 5, 2008, pages 175 - 177
ODA STOEVESANDT; MICHAEL J. TAUSSING; MINGYUE HE: "Protein Microarrays: High-Throughput Tools for Proteomics", EXPERT REVIEW OF PROTEOMICS, vol. 6, 2009, pages 145 - 157
PAULO P. AMARAL; MARCEL E. DINGER; TIM R. MERCER; JOHN S. MATTICK: "The Eukaryotic Genome as an RNA Machine", SCIENCE, vol. 319, no. 28, 2008, pages 1787 - 1789
SAKAI-KATO K. ET AL.: "An enzyme-immobilization method for integration of biofunctions on a microchip using a water-soluble amphiphilic phospholipid polymer having a reacting group.", LAB CHIP, vol. 4, 2004, pages 4 - 6, XP008159168
XU Y. ET AL.: "Microfluidic flow control on charged phospholipid polymer interface.", LAB CHIP, vol. 7, 2007, pages 199 - 206, XP008159120
KAZUHIKO ISHIHARA ET AL.: "Bio Device Hyomen no Nano Seimitsu Sosei", GEKKAN FUNCTION & MATERIALS, vol. 27, no. 5, 2007, pages 7 - 15, XP008159572
MADOKA TAKAI: "Bio Device Sosei no Tameno Kinosei Nano Interface", MATERIAL INTEGRATION, vol. 20, no. 9, 2007, pages 44 - 49, XP008159117
RUNGPRAGAYPHAN S. ET AL.: "High-throughput, cloning-independent protein library construction by combining single-molecule DNA amplification with in vitro expression.", J. MOL. BIOL., vol. 318, 2002, pages 395 - 405, XP008159142
RUNGPRAGAYPHAN S. ET AL.: "Rapid screening for affinity-improved scFvs by means of single- molecule-PCR-linked in vitro expression.", J. MOL. CATAL. B ENZYM., vol. 28, 2004, pages 223 - 228, XP008159207
KOGA Y. ET AL.: "In vitro construction and screening of a Burkholderia cepacia lipase library using single-molecule PCR and cell-free protein synthesis.", J. BIOSCI. BIOENG., vol. 94, 2002, pages 84 - 86, XP008159635
KOGA Y. ET AL.: "Inverting enantioselectivity of Burkholderia cepacia KWI-56 lipase by combinatorial mutation and high-throughput screening using single-molecule PCR and in vitro expression.", J. MOL. BIOL., vol. 331, 2003, pages 585 - 592, XP008159202
EIICHI TAMITANI: "DNA1 Bunshi kara no Protein Device eno Shin Tenkai", CHEMISTRY, vol. 57, no. 12, 2002, pages 37 - 38, XP008159548
NAKANO H. ET AL.: "Single-step single-molecule PCR of DNA with a homo-priming sequence using a single primer and hot-startable DNA polymerase.", J. BIOSCI. BIOENG., vol. 90, 2000, pages 456 - 458, XP008159244
See also references of EP 2511378A4
ANDREI KOROSTELEV; HARRY F. NOLLER: "The Ribosome in Focus: New Structures bring New Insights", TRENDS IN BIOCHEMICAL SCIENCES, vol. 32, no. 9, 2007, pages 434 - 441
R. ANDREW MARSHALL; COLLIN ECHEVERRIA AITKEN; MAGDALENA DORYWALSKA; JOSEPH D. PUGLISI: "Translation at the Single-Molecule Level", ANNUAL REVIEW OF BIOCHEMISTRY, vol. 77, 2008, pages 177 - 203
BORIS ROTMAN: "Measurement of Activity of Single Molecules of (3-D-Galactosidase", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 47, 1961, pages 1981 - 1991
DAN S. TAWFIK; ANDREW D. GRIFFITHS: "Man-Made Cell-Like Compartments for Molecular Evolution", NATURE BIOTECHNOLOGY, vol. 16, 1998, pages 652 - 656
YANNICK RONDELEZ; GUILLAUME TRESSET; KAZUHITO V. TABATA; HIDEYUKI ARATA; HIROYUKI FUJITA; SHOJI TAKEUCHI; HIROYUKI NOJI: "Microfabricated arrays of femtoliter chambers allow single molecule enzymology", NATURE BIOTECHNOLOGY, vol. 23, 2005, pages 361 - 365
TAKESHI KINPARA; RYUTA MIZUNO; YUJI MURAKAMI; MASAAKI KOBAYASHI; SHOHEI YAMAURA; QUAMRUL HASAN; YASUTSUKA MORITA; HIDEO NAKANO; TS: "A Picoliter Chamber Array for Cell-Free Protein Synthesis", JOURNAL OF BIOCHEMISTRY, vol. 136, no. 2, 2004, pages 149 - 154
TAKAOKI YAMAMOTO; TAKAHIKO NOJIMA; TERUO FUJII: "PDMS-Glass Hybrid Microreactor Array with embedded Temperature Control Device. Application to Cell-Free Protein Synthesis", LAB ON A CHIP, vol. 2, 2002, pages 197 - 202
MARI TABUCHI; MAMI HINO; YASUO SHINOHARA; YOSHINOBU BABA: "Cell-Free Protein Synthesis on a Microchip", PROTEOMICS, vol. 2, 2002, pages 430 - 435
PHILIPP ANGENENDT; LAJOS NYARSIK; WITOLD SZAFLARSKI; JORN GLOKLER; KNUD H. NIERHAUS; HANS LEHRACH; DOLORES J. CAHILL; ANGELIKA LUE: "Cell-Free Protein Expression and Functional Assay in Nanowell Chip Format", ANALYTICAL CHEMISTRY, vol. 76, no. 7, 2004, pages 1844 - 1849
PHILIPP ANGENENDT; JURGEN KREUTZBERGER; JORN GLOKLER; JORD D. HOHEISEL: "Generation of High Density Protein Microarrays by Cell-Free in Situ Expression of Unpurified PCR Products", MOLECULAR & CELLULAR PROTEOMICS, vol. 5, no. 9, 2006, pages 1658 - 1666
SHENG-CE TAO; HENG ZHU: "Protein Chip Fabrication by Capture of Nascent Polypeptides", NATURE BIOTECHNOLOGY, vol. 24, no. 10, 2006, pages 1253 - 1254
NIROSHAN RAMACHANDRAN; JACOB V. RAPHAEL; EUGENIE HAINSWORTH; GOKHAN DEMIRKAN; MANUEL G. FUENTES; ANDREAS ROLFS; YANHUI HU; JOSHUA: "Next-Generation High-Density Self-Assembling Functional Protein Arrays", NATURE METHODS, vol. 5, 2008, pages 535 - 538
MINGYUE HE; ODA STOEVESANDT; ELIZABETH A. PALMER; FARID KHAN; OLLE ERICSSON; MICHAEL TAUSSIG: "Printing Protein Arrays from DNA Arrays", NATURE METHODS, vol. 5, 2008, pages 175 - 177
ODA STOEVESANDT; MICHAEL J. TAUSSING; MINGYUE HE: "Protein Microarrays: High-Throughput Tools for Proteomics", EXPERT REVIEW OF PROTEOMICS, vol. 6, 2009, pages 145 - 157
PAULO P. AMARAL; MARCEL E. DINGER; TIM R. MERCER; JOHN S. MATTICK: "The Eukaryotic Genome as an RNA Machine", SCIENCE, vol. 319, no. 28, 2008, pages 1787 - 1789
Attorney, Agent or Firm:
SHIMIZU, MAMORU (JP)
Spring water 守 (JP)
Spring water 守 (JP)
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