Title:
METHOD OF EXPRESSING LONG−CHAIN PRENYL DIPHOSPHATE SYNTHASE
Document Type and Number:
WIPO Patent Application WO/2002/092811
Kind Code:
A1
Abstract:
A process for producing a long−chain prenyl diphosphate synthase (in particular, decaprenyl diphosphate synthase or solanesyl diphosphate synthase) by using a gene and a protein which are required for expressing or enhancing the enzymatic activity of the long−chain prenyl diphosphate synthase originating in an eucaryote&semi and a process for efficiently producing a coenzyme Q having a long−chain isoprenoid in its side chain (in particular, coenzyme Q9 or coenzyme Q10) with the use of a microorganism. DNA having a base sequence represented by SEQ ID NO:1, 3 or 5, or a DNA sequence derived therefrom by deletion, addition, insertion and&sol or substitution of one or several bases and encoding a protein which is capable of expressing (functionizing)or enhancing the activity of an eucaryote−origin long−chain prenyl diphosphate synthase in a host microorganism.
Inventors:
MATSUDA HIDEYUKI (JP)
KAWAMUKAI MAKOTO (JP)
YAJIMA KAZUYOSHI (JP)
KAWAMUKAI MAKOTO (JP)
YAJIMA KAZUYOSHI (JP)
Application Number:
PCT/JP2002/004566
Publication Date:
November 21, 2002
Filing Date:
May 10, 2002
Export Citation:
Assignee:
KANEKA CORP (JP)
MATSUDA HIDEYUKI (JP)
KAWAMUKAI MAKOTO (JP)
YAJIMA KAZUYOSHI (JP)
MATSUDA HIDEYUKI (JP)
KAWAMUKAI MAKOTO (JP)
YAJIMA KAZUYOSHI (JP)
International Classes:
C12N1/21; C12N9/10; C12P7/66; (IPC1-7): C12N15/09; C07K14/39; C07K14/47; C12N1/21; C12P21/02
Domestic Patent References:
| WO2001014567A1 | 2001-03-01 |
Foreign References:
| US6225097B1 | 2001-05-01 | |||
| JP2002191367A | 2002-07-09 |
Other References:
SUZUKI K. ET AL.: "Analysis of the decaprenyl diphosphate synthase (dps) gene in fission yeast suggests a role of ubiquinone as an antioxidant", J. BIOCHEM., vol. 121, no. 3, March 1997 (1997-03-01), pages 496 - 505, XP002935174
OKADA K. ET AL.: "Molecular cloning and mutational analysis of the ddsA gene encoding decaprenyl diphosphate synthase from gluconobacter suboxydans", EUR. J. BIOCHEM., vol. 255, April 1998 (1998-04-01), pages 52 - 59, XP002928524
OKADA K. ET AL.: "Cloning of the sdsA gene encoding solanesyl diphosphate synthase from phodobacter capsulatus and its functional expression in escherichia coli and saccharomyces cerevisiae", J. BACTERIOL., vol. 179, no. 19, October 1997 (1997-10-01), pages 5992 - 5998, XP002957170
See also references of EP 1386964A4
OKADA K. ET AL.: "Molecular cloning and mutational analysis of the ddsA gene encoding decaprenyl diphosphate synthase from gluconobacter suboxydans", EUR. J. BIOCHEM., vol. 255, April 1998 (1998-04-01), pages 52 - 59, XP002928524
OKADA K. ET AL.: "Cloning of the sdsA gene encoding solanesyl diphosphate synthase from phodobacter capsulatus and its functional expression in escherichia coli and saccharomyces cerevisiae", J. BACTERIOL., vol. 179, no. 19, October 1997 (1997-10-01), pages 5992 - 5998, XP002957170
See also references of EP 1386964A4
Attorney, Agent or Firm:
Yasutomi, Yasuo (4-20 Nishinakajima 5-chome, Yodogawa-k, Osaka-shi Osaka, JP)
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