Title:
METHOD FOR EXTRACTING COLLAGEN TRIPEPTIDE AND PREPARED COLLAGEN TRIPEPTIDE
Document Type and Number:
WIPO Patent Application WO/2023/040084
Kind Code:
A1
Abstract:
A method for extracting collagen tripeptide, a collagen tripeptide prepared using said method, and an application thereof in preparing an antioxidant product. The preparation method comprises: crushing fish skin, then washing the fish skin with a NaOH solution, distilled water, n-hexane, and distilled water; mixing the washed fish skin with water, swelling and homogenizing, adding alkaline protease and papain in a mass ratio of 1:3, the total amount of enzymes being 110 U/g, performing enzymolysis at a pH value of 7.5 and a temperature 75°C for 6 hours, and then performing ultrasonic treatment at 600 W for 45 minutes; performing enzyme deactivation and centrifugation on the solution obtained after ultrasonic treatment, and collecting the supernatant to obtain collagen tripeptide.
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Inventors:
ZHAO ZIFANG (CN)
GUO HONGXING (CN)
ZHOU JINXUE (CN)
GUO HONGXING (CN)
ZHOU JINXUE (CN)
Application Number:
PCT/CN2021/137047
Publication Date:
March 23, 2023
Filing Date:
December 10, 2021
Export Citation:
Assignee:
HAINAN SAN YUAN XING BIOTECHNOLOGY CO LTD (CN)
International Classes:
C07K5/083; A61P39/06; C07K14/78; C12G3/02; C12P21/06
Foreign References:
CN102242176A | 2011-11-16 | |||
CN109336966A | 2019-02-15 | |||
CN101870995A | 2010-10-27 | |||
CN1903918A | 2007-01-31 | |||
CN104498568A | 2015-04-08 | |||
CN102676619A | 2012-09-19 |
Attorney, Agent or Firm:
UNITALEN ATTORNEYS AT LAW (CN)
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