Title:
METHOD FOR FUNCTIONALLY SCREENING AGENTS WHICH CAN MODULATE THE ACTIVITY OF UBIQUITINE HYDROLASES AND USES THEREOF.
Document Type and Number:
WIPO Patent Application WO2005028613
Kind Code:
A3
Abstract:
A cell-screening method for compounds which can modulate the activity of ubiquitine hydrolases comprising the following stages: cultivation of a strain of modified yeast in a rich medium, comprising a hybrid sequence containing a sequance coding for a protein (Met4) and a transcriptional reporter system containing a a yeast promoter activated by the transcriptional factor (Met4), the transfer of the culture into a rich medium over a period of time enabling expression of the protein (Met4), transfer of the culture into a medium which does not enable expression of the protein (Met4), bringing the strain of yeast thus cultivated into contact with a test compound, the addition of cadmium, and determination of the level of expression of the hybrid protein expressed by a promoter MET.
Inventors:
THOMAS DOMINIQUE (FR)
BARBEY REGINE (FR)
BARBEY REGINE (FR)
Application Number:
PCT/FR2004/002221
Publication Date:
June 16, 2005
Filing Date:
September 01, 2004
Export Citation:
Assignee:
CENTRE NAT RECH SCIENT (FR)
THOMAS DOMINIQUE (FR)
BARBEY REGINE (FR)
THOMAS DOMINIQUE (FR)
BARBEY REGINE (FR)
International Classes:
C12Q1/34; G01N33/68; (IPC1-7): G01N33/68; C12Q1/34; C12Q1/68
Domestic Patent References:
| WO2001040499A2 | 2001-06-07 | |||
| WO1999004033A1 | 1999-01-28 | |||
| WO2002008394A2 | 2002-01-31 | |||
| WO1997012962A1 | 1997-04-10 | |||
| WO1999038969A1 | 1999-08-05 | |||
| WO1999018989A1 | 1999-04-22 |
Foreign References:
| US5932425A | 1999-08-03 |
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