METHOD OF GENERATING LONG NUCLEIC ACID MOLECULES OF DEFINED SEQUENCE

Title:

METHOD OF GENERATING LONG NUCLEIC ACID MOLECULES OF DEFINED SEQUENCE

Document Type and Number:

WIPO Patent Application WO2004092330

Kind Code:

A3

Abstract:

A method of generating a single-stranded nucleic acid of a predetermined sequence, comprising: a) contacting a poynucleotide comprising a 5' portion and a 3' portion, a template oligonucleotide having a 3' blocker, a 5' template portion, and a 3' portion which is sufficiently complementary to the 3' portion of the polynucleotide to hybridize thereto, and a polymerase under conditions in which the 3' portion of the polynucleotide hybridizes to the 3' portion of the template oligonucleotide and elongates to produce an elongated polynucleotide product; b) denaturing the duplex comprising the elongation product and a template oligonucleotide; c) repeating a) and b) in the presence of a second or subsequent template oligonucleotide to produce a second or subsequent elongation polynucleotide product.

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Inventors:

CHANG CHU-AN (US)
CHEN SHIAW-MIN (US)

Application Number:

PCT/US2004/010614

Publication Date:

November 16, 2006

Filing Date:

April 07, 2004

Export Citation:

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Assignee:

APPLERA CORP (US)
CHANG CHU-AN (US)
CHEN SHIAW-MIN (US)

International Classes:

C12P19/34; C07H21/02; C12N9/00; C12Q1/68; C12N

Foreign References:

US20020119535A1	2002-08-29
JPH09149789A	1997-06-10
US20010049125A1	2001-12-06
US6218118B1	2001-04-17

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