Title:
METHOD OF GENERATING SIZE STANDARD NUCLEIC ACIDS
Document Type and Number:
WIPO Patent Application WO2004092327
Kind Code:
A3
Abstract:
Methods for generating nucleic acid size standards are disclosed. The methods comprise providing a template polynucleotide which comprises periodic sequences of from about 5 to about 50 contiguous nucleotides containing not more than three types of nucleotides and wherein adjacent periodic sequences are separated by a terminator complement nucleotide that differs from the not more than three types of nucleotides, and performing a primer extension reaction on the template polynucleotide in the presence of nucleoside triphosphate molecules and a 3' terminating nucleoside triphosphate which is complementary to the terminator complement nucleotide.
Inventors:
KUO SOPHIA S (US)
CHEN SHIAW-MIN (US)
CHANG CHU-AN (US)
SPURGEON SANDRA L (US)
CHEN SHIAW-MIN (US)
CHANG CHU-AN (US)
SPURGEON SANDRA L (US)
Application Number:
PCT/US2004/010601
Publication Date:
October 13, 2005
Filing Date:
April 07, 2004
Export Citation:
Assignee:
APPLERA CORP (US)
KUO SOPHIA S (US)
CHEN SHIAW-MIN (US)
CHANG CHU-AN (US)
SPURGEON SANDRA L (US)
KUO SOPHIA S (US)
CHEN SHIAW-MIN (US)
CHANG CHU-AN (US)
SPURGEON SANDRA L (US)
International Classes:
C07H21/00; C12P19/34; C12Q1/68; C12N; (IPC1-7): C12Q1/68; C07H21/00
Foreign References:
| US20020045178A1 | 2002-04-18 | |||
| US6448407B1 | 2002-09-10 |
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