Title:
METHOD FOR IMPROVING ADHESION FORCE OF RECOMBINANT MUSSEL FOOT PROTEIN
Document Type and Number:
WIPO Patent Application WO/2023/245971
Kind Code:
A1
Abstract:
Disclosed is a method for improving adhesion force of a recombinant mussel foot protein. According to the method, by means of bioinformatics and molecular dynamics simulation, solvent accessibility of protein molecule amino acid residues is calculated, and site-directed mutation is carried out on residue sites. In the present invention, after amino acids at sites N33, N46, and N62 of a wild-type Mytilus coruscus type-3 protein Mcofp-3A, sites G41 and N44 of a wild-type Mytilus galloprovincalis type-3A protein Mgfp-3A, and sites G33, N43, and N76 of a wild-type Mytilus galloprovincalis type-3B protein Mgfp-3B are each mutated into tyrosine that has an adhesion effect on the mussel foot protein, the mutant proteins have no obvious changes in expression, and they have an amino acid sequence set forth in any one of SEQ ID NO.1-8 and have improved adhesion performance with the adhesion force improved by 16%-38% and 12%-82% after liagation for 18 hours in an aqueous solution and a saline solution, respectively, compared with the adhesion force of the original proteins.
Inventors:
LI SHA (CN)
XUE RUI (CN)
XU HONG (CN)
FENG XINXIN (CN)
WANG RUI (CN)
HUANG WEIWEI (CN)
XUE RUI (CN)
XU HONG (CN)
FENG XINXIN (CN)
WANG RUI (CN)
HUANG WEIWEI (CN)
Application Number:
PCT/CN2022/132226
Publication Date:
December 28, 2023
Filing Date:
November 16, 2022
Export Citation:
Assignee:
UNIV NANJING TECH (CN)
International Classes:
C07K14/435; A61L24/10; C07K1/22; C12N1/21; C12N15/12; C12N15/70; G16B20/50; G16C10/00
Domestic Patent References:
| WO2022046932A2 | 2022-03-03 |
Foreign References:
| CN115181169A | 2022-10-14 | |||
| CN101948519A | 2011-01-19 | |||
| CN108218970A | 2018-06-29 | |||
| CN108395483A | 2018-08-14 | |||
| CN108503712A | 2018-09-07 | |||
| CN101429508A | 2009-05-13 |
Other References:
DATABASE Protein 25 January 2010 (2010-01-25), ANONYMOUS : "foot protein 3 variant 5 [Mytilus coruscus] ", XP093121026, retrieved from NCBI Database accession no. ADB79742.1
DATABASE Protein 25 January 2010 (2010-01-25), ANONYMOUS : "foot protein 3 variant 4 [Mytilus coruscus]", XP093121029, retrieved from NCBI Database accession no. ADB79741.1
GENBANK ACCESSION NUMBER BAB16315.1: "04 October 2000 (04.10.2000), [searched on 03 March 2023 (03.03.2023)]", SEARCHED FROM NCBI [ONLINE]: HTTPS://WWW.NCBI.NLM.NIH.GOV/PROTEIN/BAB16315.1?REPORT=GENBANK&LOG$=PROTALIGN&BLAST_RANK=1&RID=0AUGRCAT013, 4 October 2000 (2000-10-04)
DATABASE Protein 10 October 2020 (2020-10-10), ANONYMOUS : "Hypothetical predicted protein [Mytilus galloprovincialis] ", XP093121034, retrieved from NCBI Database accession no. VDI05014.1
DATABASE Protein 4 October 2000 (2000-10-04), ANONYMOUS : "foot protein-3A [Mytilus galloprovincialis] ", XP093121037, retrieved from NCBI Database accession no. BAB16314.1
DATABASE Protein 30 July 2000 (2000-07-30), ANONYMOUS : "foot protein-3 precursor variant13 [Mytilus edulis]", XP093121039, retrieved from NCBI Database accession no. AAF89292.1
贺存学 (HE, CUNXUE): "分子动力学研究蛋白质的吸附行为 (Molecular Dynamics Study on the Adsorption Behaviors of Protein)", 中国优秀硕士学位论文全文数据库(电子期刊)工程科技I辑 (CHINESE MASTER'S THESES FULL-TEXT DATABASE (ELECTRONIC JOURNALS), ENGINEERING SCIENCE AND TECHNOLOGY I), no. 10, 15 October 2017 (2017-10-15)
DATABASE Protein 25 January 2010 (2010-01-25), ANONYMOUS : "foot protein 3 variant 4 [Mytilus coruscus]", XP093121029, retrieved from NCBI Database accession no. ADB79741.1
GENBANK ACCESSION NUMBER BAB16315.1: "04 October 2000 (04.10.2000), [searched on 03 March 2023 (03.03.2023)]", SEARCHED FROM NCBI [ONLINE]: HTTPS://WWW.NCBI.NLM.NIH.GOV/PROTEIN/BAB16315.1?REPORT=GENBANK&LOG$=PROTALIGN&BLAST_RANK=1&RID=0AUGRCAT013, 4 October 2000 (2000-10-04)
DATABASE Protein 10 October 2020 (2020-10-10), ANONYMOUS : "Hypothetical predicted protein [Mytilus galloprovincialis] ", XP093121034, retrieved from NCBI Database accession no. VDI05014.1
DATABASE Protein 4 October 2000 (2000-10-04), ANONYMOUS : "foot protein-3A [Mytilus galloprovincialis] ", XP093121037, retrieved from NCBI Database accession no. BAB16314.1
DATABASE Protein 30 July 2000 (2000-07-30), ANONYMOUS : "foot protein-3 precursor variant13 [Mytilus edulis]", XP093121039, retrieved from NCBI Database accession no. AAF89292.1
贺存学 (HE, CUNXUE): "分子动力学研究蛋白质的吸附行为 (Molecular Dynamics Study on the Adsorption Behaviors of Protein)", 中国优秀硕士学位论文全文数据库(电子期刊)工程科技I辑 (CHINESE MASTER'S THESES FULL-TEXT DATABASE (ELECTRONIC JOURNALS), ENGINEERING SCIENCE AND TECHNOLOGY I), no. 10, 15 October 2017 (2017-10-15)
Attorney, Agent or Firm:
JIANGSU SUNDY LAW FIRM (CN)
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