Title:
METHOD FOR INDUCING AND PROLIFERATING NATURAL KILLER CELLS DERIVED FROM PERIPHERAL BLOOD MONONUCLEAR CELLS
Document Type and Number:
WIPO Patent Application WO/2013/168978
Kind Code:
A1
Abstract:
The present invention relates to a method for inducing and proliferating natural killer cells derived from peripheral blood mononuclear cells, the method comprising co-culturing, as vegetative cells, irradiated Jurkat cells and irradiated Epstein-Barr virus transformed lymphocyte continuous line (EBV-LCL) cells in the presence of cytokines, along with peripheral blood mononuclear cells. According to the present invention, a large quantity of natural killer cells can be induced and proliferated from a small quantity of peripheral blood mononuclear cells even without the use of high-cost equipment or cytokines, thereby making it possible to significantly improve the efficiency and efficacy of using the natural killer cells for the prevention and treatment of cancer.
Inventors:
LEE KYUNG MI (KR)
LIM SEON AH (KR)
YEE CASSIAN (KR)
LIM SEON AH (KR)
YEE CASSIAN (KR)
Application Number:
PCT/KR2013/003981
Publication Date:
November 14, 2013
Filing Date:
May 07, 2013
Export Citation:
Assignee:
UNIV KOREA RES & BUS FOUND (KR)
International Classes:
C12N5/0783; A61K35/12; A61K35/14; A61K39/00; C12N13/00
Domestic Patent References:
| WO2006050270A2 | 2006-05-11 | |||
| WO2008118369A2 | 2008-10-02 |
Foreign References:
| KR20120016427A | 2012-02-24 | |||
| US20080166326A1 | 2008-07-10 | |||
| US20110135687A1 | 2011-06-09 |
Other References:
LECERDA, JOAO F. ET AL.: "Human Epstein-Barr virus (EBV)-specific cytotoxic T lymphocytes home preferentially to and induce selective regressions of autologous EBV- induced B cell lymphoproliferations in xenografted C.B-17 Scid/Scid mice", THE JOURNAL OF EXPERIMENTAL MEDICINE, vol. 183, no. 3, 1 March 1996 (1996-03-01), pages 1215 - 1228
Attorney, Agent or Firm:
DANA PATENT LAW FIRM (KR)
특허법인 다나 (KR)
특허법인 다나 (KR)
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