Title:
METHOD FOR INTRODUCING FOREIGN SUBSTANCE INTO CELL USING LASER
Document Type and Number:
WIPO Patent Application WO/2018/105748
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a method which enables the introduction of a foreign substance into a single cell with high efficiency and easily and is less likely to be influenced by the damage of the cell. A cell is left standing on a thin film which coats the upper surface of a coverslip and which is made from any one metal selected from gold, platinum, silver and aluminum, then a liquid containing a foreign substance to be introduced is brought into contact with the cell, and then laser light is emitted onto the thin film adjacent to the cell to cause the absorption of the laser light by the thin film. As a result, a fine pore is formed in the cell membrane, and the foreign substance is introduced into the cell through the fine pore.
Inventors:
YUMURA SHIGEHIKO (JP)
Application Number:
PCT/JP2017/044259
Publication Date:
June 14, 2018
Filing Date:
December 08, 2017
Export Citation:
Assignee:
UNIV YAMAGUCHI (JP)
International Classes:
C12N13/00; C12M1/00; C12N15/09
Domestic Patent References:
| WO2015170758A1 | 2015-11-12 |
Foreign References:
| JP2014513984A | 2014-06-19 | |||
| JP2015167551A | 2015-09-28 |
Attorney, Agent or Firm:
FUJIMOTO Shohei (JP)
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