Title:
METHOD FOR ISOLATING PHYSIOLOGICALLY ACTIVE SUBSTANCE USING TEMPERATURE-RESPONSIVE LIGAND IMMOBILIZED MEMBRANE MODULE
Document Type and Number:
WIPO Patent Application WO/2012/086838
Kind Code:
A1
Abstract:
In this method for isolating a physiologically active substance using a membrane module to which is immobilized a temperature-responsive ligand of which the affinity to the physiologically active substance changes with changes in temperature, the membrane module has a primary side at which a solution containing a physiologically active substance is supplied and a secondary side from which the solution that has passed through the membrane module is discharged. The temperature of the membrane module is altered by means of the following steps (a) to (c): (a) a step wherein the physiologically active substance is adsorbed to the membrane by passing the solution containing the physiologically active substance through the membrane to which the temperature-responsive ligand has been immobilized; (b) a step wherein the primary side of the membrane module is replaced with a liquid at a temperature at which the physiologically active substance elutes from the membrane; and (c) a step wherein the liquid that is at a temperature at which the physiologically active substance elutes is passed through the membrane to elute the physiologically active substance from the membrane.
Inventors:
KOGUMA ICHIRO (JP)
OKUYAMA KAZUO (JP)
OKUYAMA KAZUO (JP)
Application Number:
PCT/JP2011/080068
Publication Date:
June 28, 2012
Filing Date:
December 26, 2011
Export Citation:
Assignee:
ASAHI KASEI MEDICAL CO LTD (JP)
KOGUMA ICHIRO (JP)
OKUYAMA KAZUO (JP)
KOGUMA ICHIRO (JP)
OKUYAMA KAZUO (JP)
International Classes:
C07K1/22; B01D69/02; B01D69/06; B01D69/08; C07K1/14
Domestic Patent References:
| WO2008143199A1 | 2008-11-27 | |||
| WO1999061904A1 | 1999-12-02 |
Foreign References:
| US20050208637A1 | 2005-09-22 | |||
| JP2006026607A | 2006-02-02 |
Other References:
MANDARO R.M. ET AL.: "Filtration Supports for Affinty Separation", BIO/TECHNOLOGY, vol. 5, 1987, pages 928, 931 - 932
Attorney, Agent or Firm:
INABA, Yoshiyuki et al. (JP)
Yoshiyuki Inaba (JP)
Yoshiyuki Inaba (JP)
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Claims: