Title:
METHOD OF ISOLATING AND PURIFYING FUSION PROTEIN COMPRISING FACTOR VII
Document Type and Number:
WIPO Patent Application WO/2014/129876
Kind Code:
A1
Abstract:
The present invention relates to a method of isolating and purifying a fusion protein comprising factor VII, and more specifically relates to a method of isolating and purifying a fusion protein comprising factor VII and transferrin, to a high degree of purity. Because the present invention provides a method whereby a recombinant fusion protein comprising factor VII can be isolated and purified to a high degree of purity, the invention is useful in producing a pharmaceutical preparation comprising factor VII that can be used in situations in which copious bleeding occurs such as surgery.
Inventors:
LEE JI-HYE (KR)
KANG SEOK-CHAN (KR)
RYU YANGKYUN (KR)
LEE HO SOON (KR)
SONG IN-YOUNG (KR)
KIM HUN-TAEK (KR)
KANG SEOK-CHAN (KR)
RYU YANGKYUN (KR)
LEE HO SOON (KR)
SONG IN-YOUNG (KR)
KIM HUN-TAEK (KR)
Application Number:
PCT/KR2014/001526
Publication Date:
August 28, 2014
Filing Date:
February 25, 2014
Export Citation:
Assignee:
SK CHEMICALS CO LTD (KR)
International Classes:
C07K1/16; C07K14/745; C07K19/00
Foreign References:
| KR20110133454A | 2011-12-12 | |||
| US20090170163A1 | 2009-07-02 |
Other References:
"Internet Printout related to VIISelect.", GE HEALTHCARE LIFE SCIENCES., October 2011 (2011-10-01)
EMANUELSSON, I. ET AL., CHARACTERISING OF CHROMATOGRAPHY GELS FOR PURIFICATION OF ERYTHROPOIETIN., 2008, UNIVERSITY OF BORAS/SCHOOL OF ENGINEERING., XP055280502
GUHA, A. ET AL.: "Affinity purification of human tissue factor: interaction of factor VII and tissue factor in detergent micelles.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 83, no. 2, January 1986 (1986-01-01), pages 299 - 302, XP055280504
HALABIAN, R. ET AL.: "Expression and purification of recombinant human coagulation factor VII fused to a histidine tag using Gateway technology.", BLOOD TRANSFUSION., vol. 7, no. 4, October 2009 (2009-10-01), pages 305 - 312, XP055070586
MORI, F. ET AL.: "Progress in large-scale purification of factor VIII/von Willebrand factor concentrates using ion-exchange chromatography.", VOX SANGUINIS., vol. 95, no. 4, November 2008 (2008-11-01), pages 298 - 307, XP055280506
HAGEN FS ET AL., PROC. NATL. ACAD. SCI., USA, vol. 83, no. 8, 1986, pages 2412 - 2416
See also references of EP 2960248A4
EMANUELSSON, I. ET AL., CHARACTERISING OF CHROMATOGRAPHY GELS FOR PURIFICATION OF ERYTHROPOIETIN., 2008, UNIVERSITY OF BORAS/SCHOOL OF ENGINEERING., XP055280502
GUHA, A. ET AL.: "Affinity purification of human tissue factor: interaction of factor VII and tissue factor in detergent micelles.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 83, no. 2, January 1986 (1986-01-01), pages 299 - 302, XP055280504
HALABIAN, R. ET AL.: "Expression and purification of recombinant human coagulation factor VII fused to a histidine tag using Gateway technology.", BLOOD TRANSFUSION., vol. 7, no. 4, October 2009 (2009-10-01), pages 305 - 312, XP055070586
MORI, F. ET AL.: "Progress in large-scale purification of factor VIII/von Willebrand factor concentrates using ion-exchange chromatography.", VOX SANGUINIS., vol. 95, no. 4, November 2008 (2008-11-01), pages 298 - 307, XP055280506
HAGEN FS ET AL., PROC. NATL. ACAD. SCI., USA, vol. 83, no. 8, 1986, pages 2412 - 2416
See also references of EP 2960248A4
Attorney, Agent or Firm:
FIRSTLAW P.C. (Seocho-Ku, Seoul 137-739, KR)
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